Inhibition of Alzheimer Amyloid Aggregation with Sulfate Glycopolymers
Glycopolymers carrying sulfate saccharides were found to suppress the formation of amyloid fibrils by amyloid beta peptides, as evaluated by fluorescence assay of thioflavin T and AFM. CD spectra showed that the conformation of amyloid beta peptides was changed from beta peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the β-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect.
Pietro VINCENZINI and Danilo DE ROSSI
Y. Miura et al., "Inhibition of Alzheimer Amyloid Aggregation with Sulfate Glycopolymers", Advances in Science and Technology, Vol. 57, pp. 166-169, 2008