Recently, Ti-based metallic glasses aim at biomaterials with their high specific strength and superior corrosion resistance. Their high workability also shows a good performance for mass production under the energy saving environment. In this study, we started investigation of the design of Ti-based metallic glasses with the restricted alloying elements for biocompatibility and characteristic evaluation of the optimized Ti-based metallic glasses with higher glass forming ability for dental implants. These Ti-based metallic glasses do not contain Al, V and Ni elements which are well known to be neurotoxicity and cytotoxicity for human body. Current medical reports of impracticability by these elements have been a hot issue in biomaterials science. Newly designed Ti-based metallic glasses exhibit good performances. Especially, the optimized Ti-based metallic glass has high corrosion resistance with better passivity in a wide passivation range in simulated body fluids at 310K. In addition, biocompatibility of Ti-based metallic glass was also evaluated by cell culture in vitro. Excellent biocompatibility of Ti-based metallic glass show high potentials to be applied as biomaterials that necrosis of osteoblast (SaOS2) was not detected in this study.