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3D Printing of PolyLactic Acid (PLA) Scaffold Combined with Injectable Bone Substitute (IBS) for Tuberculosis Drug Delivery

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Abstract:

Spinal tuberculosis is one of the infectious diseases which according to the World Health Organization (WHO), is a major cause of health problems and one of the top 10 causes of death worldwide. The aim of this study was to fabricate a 3D printing scaffold with the design of truncated hexahedron, then combined with Injectable Bone Substitute (IBS) paste as a method for drug delivery in the case of spinal tuberculosis. Injectable Bone Substitute (IBS) paste was synthesized by combining some materials including hydroxyapatite, gelatin, hydroxypropyl methylcellulose (HPMC), and streptomycin. The scaffold was characterized with IBS paste through the digital microscope and the mechanical test to determine the mechanical strength of the scaffold. The results of the 3D printing scaffold showed that the scaffold has interconnectivity between pores. After being injected with IBS, it was seen that the entire surface of the scaffold pores was covered by IBS paste evenly. Scanning Electron Microscope (SEM) tests showed that the surface of the scaffold has been covered by IBS paste, and proves that the pores are still formed. Energy Dispersive X-Ray (EDX) test results showed that the IBS paste containing a hydroxyapatite component consisting of Ca, P, and O elements. Mechanical tests showed that the scaffold for all pore sizes had a compressive strength of 1.49-3.97 MPa before IBS injection and increased to 3.45-4.77 MPa after IBS injection. Then the bending test showed that the scaffold had a bending strength of 16.76-36.09 MPa and increased to around 21.57-40.36 MPa after being injected with IBS. The drug release test showed that the 3D printing scaffold could release streptomycin by 4.944%-6.547%, which has met the percentage of drug release that is able to kill tuberculosis bacteria. It can be concluded that 3D printing scaffold combined with IBS paste can be applied as a drug carrier as well as a method of healing spinal tuberculosis.

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Periodical:

Diffusion Foundations and Materials Applications (Volume 33)

Pages:

73-83

DOI:

https://doi.org/10.4028/p-c0lw62

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Online since:

April 2023

Authors:

Dyah Hikmawati*, Aniek Setiya Budiatin, Aminatun Aminatun, Eka Yuliatin, Frazna Parastuti, Prihartini Widiyanti

Keywords:

3D Printing Scaffold, Anti-Tuberculosis, Drug Delivery, Injectable Bone Substitute

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© 2023 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] World Health Organization (WHO) global tuberculosis report accessed from https://www.who.int/publications/i/item/9789240013131. accessed on 8th May 2022 at 09.20 PM.

Google Scholar

[2] TB Indonesia accessed from https://tbindonesia.or.id/pustaka-tbc/dashboard-tb/ accessed on 12nd July 2022 at 09.02 PM.

Google Scholar

[3] Liang, X., Weiyang, Z., Zhengxue, Q., Xiao-Ji, L., and Dian-Ming, J. One-Stage Posterior Debridement with Transverse Process Strut as Bone Graft in The Surgical Treatment of Single-Segment Thoracic Tuberculosis. Medicine. 2019. 98 (47). https://journals.lww.com/md-journal/Fulltext/2019/11220/One_stage_posterior_debridement_with_transverse.50.aspx

DOI: 10.1097/md.0000000000018022

Google Scholar

[4] Hikmawati, D., Maulida, H.N., Putra, A.P., Budiatin, A.S., Syahrom. Synthesis and Characterization of Nanohydroxyapatite-Gelatin Composite with Streptomycin as Antituberculosis Injectable Bone Substitute. International Journal of Biomaterials, 2019, Vol. 2019. https://www.hindawi.com/journals/ijbm/2019/7179243/

DOI: 10.1155/2019/7179243

Google Scholar

[5] Li, Y., Yu, B., Ai, F., Wu, C., Zhou, K., Cao, C., Li, W., Characterization and evaluation of polycaprolactone/hydroxyapatite composite scaffolds with extra surface morphology by cryogenic printing for bone tissue engineering. Materials & Design, 2021, (205):109712. https://www.sciencedirect.com/science/article/pii/S0264127521002641?via%3Dihub

DOI: 10.1016/j.matdes.2021.109712

Google Scholar

[6] Sears, N.A.; Seshadri, D.R.; Dhavalikar, P.S.; Cosgriff-Hernandez, E. A review of three-dimensional printing in tissue engineering. Tissue Eng. Part B, 2016, Rev. 22, 298–310. https://www.liebertpub.com/doi/

DOI: 10.1089/ten.teb.2015.0464

Google Scholar

[7] Vyas, C.; Zhang, J.; Øvrebø, Ø.; Huang, B.; Roberts, I.; Setty, M.; Allardyce, B.; Haugen, H.; Rajkhowa, R.; Bartolo, P. 3D printing of silk microparticle reinforced polycaprolactone scaffolds for tissue engineering applications. Mater. Sci. Eng. C. 2020, 118, 111433. https://www.sciencedirect.com/science/article/pii/S0928493120333518?via%3Dihub

DOI: 10.1016/j.msec.2020.111433

Google Scholar

[8] Song, X.; Tetik, H.; Jirakittsonthon, T.; Parandoush, P.; Yang, G.; Lee, D.; Ryu, S.; Lei, S.; Weiss, M.L.; Lin, D. Biomimetic 3D printing of hierarchical and interconnected porous hydroxyapatite structures with high mechanical strength for bone cell culture. Adv. Eng. Mater. 2019, 21, 1800678

DOI: 10.1002/adem.201800678

Google Scholar

[9] Wichniarek, R.; Hamrol, A.; Kuczko, W.; Górski, F.; Rogalewicz, M. ABS filament moisture compensation possibilities in the FDM process. CIRP J. Manuf. Sci. Technol. 2021, 35, 550–559

DOI: 10.1016/j.cirpj.2021.08.011

Google Scholar

[10] Anitha, R.; Arunachalam, S.; Radhakrishnan, P. Critical parameters influencing the quality of prototypes in fused deposition modelling. J. Mater. Process. Technol. 2001, 118, 385–388

DOI: 10.1016/S0924-0136(01)00980-3

Google Scholar

[11] Daly, A.C.; Freeman, F.E.; Gonzalez-Fernandez, T.; Critchley, S.E.; Nulty, J.; Kelly, D.J. 3D bioprinting for cartilage and osteochondral tissue engineering. Adv. Healthc. Mater. 2017, 6, 1700298. https://onlinelibrary.wiley.com/doi/

DOI: 10.1002/adhm.201700298

Google Scholar

[12] Park, S., Kim, J.E., Han, J., Jeong, S., Lim, J.W., Lee, M.C., Son, H., Kim, H.B., Choung, Y.H., Seonwoo, H., Chung, J.H., Jang, K.J., 3D-Printed Poly(ℇ Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro. Polymers, 2021, (13):257. https://www.mdpi.com/2073-4360/13/2/257

DOI: 10.3390/polym13020257

Google Scholar

[13] Hosseinnejad, F., Imani Fooladi, A. A., Hafezi, F., Mafi, S. M., Amiri, A., & Nourani, M. R. Modelling and Tissue Engineering of Three Layers of Calvarial Bone as a Biomimetic Scaffold. Journal of Biomimetics, Biomaterials and Tissue Engineering, 2012, 15, 37–53

DOI: 10.4028/www.scientific.net/jbbte.15.37

Google Scholar

[14] Mohamed, R.M.; Yusoh, K. A review on the recent research of polycaprolactone (PCL). In Proceedings of the Advanced Materials Research; Trans Tech Publications Ltd.: Baech, Switzerland, 2016; p.249–255

DOI: 10.3390/polym13020257

Google Scholar

[15] Camargo, J.C.; Machado, Á.R.; Almeida, E.C.; Silva, E.F.M.S. Mechanical properties of PLA graphene filament for FDM 3D printing. Int. J. Adv. Manuf. Technol. 2019, 103, 2423–2443

DOI: 10.1007/s00170-019-03532-5

Google Scholar

[16] Pascual-González, C., Vega, J. de la, Thompson, C. Fernández-Blázquez, J.P., Herráez-Molinero, D., Biurrun, I. Lizarralde, N., Sánchez del Río, J., González, C., Llorca, J., Processing and mechanical properties of novel biodegradable poly-lactic acid/Zn 3D printed scaffolds for application in tissue regeneration. Journal of the Mechanical Behavior of Biomedical Materials, 2022, 132: 105290

DOI: 10.1016/j.jmbbm.2022.105290

Google Scholar

[17] Lee, S. H., Zhou, W. Y., Wang, M., Cheung, W. L., & Ip, W. Y. Selective Laser Sintering of Poly(L-Lactide) Porous Scaffolds for Bone Tissue Engineering. Journal of Biomimetics, Biomaterials and Tissue Engineering, 2008, 1, 81–89

DOI: 10.4028/www.scientific.net/jbbte.1.81

Google Scholar

[18] Karimi, A., Mole, N., Pepelnjak, T., Numerical Investigation of the Cycling Loading Behavior of 3D-Printed Poly-Lactic Acid (PLA) Cylindrical Lightweight Samples during Compression Testing. Appl. Sci. 2022, 12:8018. https://doi.org/

DOI: 10.3390/app12168018

Google Scholar

[19] Ostafinska, A.; Fortelný, I.; Hodan, J.; Krejˇcíková, S.; Nevoralová, M.; Kredatusová, J.; Kruliš, Z.; Kotek, J.; Šlouf, M. Strong synergistic effects in PLA/PCL blends: Impact of PLA matrix viscosity. J. Mech. Behav. Biomed. Mater. 2017, 69, 229–241

DOI: 10.1016/j.jmbbm.2017.01.015

Google Scholar

[20] Maulida, H.N., Hikmawati, D., Budiatin, A.S., Injectable Bone Substitute Paste Based on Hydroxyapatite, Gelatin, and Streptomycin for Spinal Tuberculosis. Journal of SCRTE, 2019, 3 (2). https://e-journal.unair.ac.id/JSCRTE/article/view/20133

DOI: 10.4172/2165-7939.1000266

Google Scholar

[21] Shavandi, A., Bekhit, A. E.-D. A., Sun, Z. F., & Ali, A. A Review of Synthesis Methods, Properties and Use of Hydroxyapatite as a Substitute of Bone. Journal of Biomimetics, Biomaterials and Biomedical Engineering, 2015, 25, 98–117

DOI: 10.4028/www.scientific.net/jbbbe.25.98

Google Scholar

[22] Liang, L.F., Han, X.Y., Yan, X.C., Weng, J., Reinforcing of Porous Hydroxyapatite Ceramics with Hydroxyapatite Fibres for Enhanced Bone Tissue Engineering. Journal of Biomimetics, Biomaterials and Tissue Engineering, 2011, 10, 67-73

DOI: 10.4028/www.scientific.net/jbbte.10.67

Google Scholar

[23] Gomez-Lizarraga, K.K.; Flores-Morales, C.; Del Prado-Audelo, M.L.; Alvarez-Perez, M.A.; Pina-Barba, M.C.; Escobedo, C. Polycaprolactone- and polycaprolactone/ceramic-based 3D-bioplotted porous scaffolds for bone regeneration: A comparative study. Mater. Sci. Eng. C Mater. Biol. Appl. 2017, 79, 326–335

DOI: 10.1016/j.msec.2017.05.003

Google Scholar

[24] Szczes, A.; Holysz, L.; Chibowski, E. Synthesis of hydroxyapatite for biomedical applications. Adv. Colloid. Interface Sci. 2017, 249, 321–330

DOI: 10.1016/j.cis.2017.04.007

Google Scholar

[25] Kim CG, Han KS, Lee S, Kim MC, Kim SY, Nah J. Fabrication of biocompatible polycaprolactone-hydroxyapatite composite filaments for the FDM 3D printing of bone scaffolds. Appl Sci 2021;11:6351. https://www.mdpi.com/2076-3417/11/14/6351

DOI: 10.3390/app11146351

Google Scholar

[26] You, B.C., Meng, C.E., Nasir, N.F.M., Tarmizi, E.Z.M., Fhan, K.S., Kheng, E.S, Majid, M.S.A., Jamir, M.R.M. Dielectric and biodegradation properties of biodegradable nano-hydroxyapatite/starch bone scaffold. Journal of materials research and technology. 2022, 18:3215-3226

DOI: 10.1016/j.jmrt.2022.04.014

Google Scholar

[27] Putra, A. P., Rahmah, A. A., Fitriana, N., Rohim, S. A., Jannah, M., & Hikmawati, D. The Effect of Glutaraldehyde on Hydroxyapatite-Gelatin Composite with Addition of Alendronate for Bone Filler Application. Journal of Biomimetics, Biomaterials and Biomedical Engineering, 2018, 37, 107–116

DOI: 10.4028/www.scientific.net/jbbbe.37.107

Google Scholar

[28] Asgari, B., Azami, M., Amiri, A., Imani Fooladi, A. A., & Nourani, M. R. Bone Scaffold Biomimetics Based on Gelatin Hydrogel Mineralization. Journal of Biomimetics, Biomaterials and Tissue Engineering, 2013, 17, 59–69

DOI: 10.4028/www.scientific.net/jbbte.17.59

Google Scholar

[29] C. Chang, C. Hu, Y. Chang. Two-stage revision arthroplasty for Mycobacterium Tuberculosis periprosthetic joint infection: an outcome analysis, PLoS ONE, 2018, vol. 13, no. 9, Article ID e0203585, p.1–13

DOI: 10.1371/journal.pone.0203585

Google Scholar

[30] Zimmerling, A., Yazdanpanah, Z., Cooper, D.M.L., Johnston, J.D., Chen, X., 3D printing PCL/nHA bone scaffolds: exploring the influence of material synthesis techniques. Biomaterials Research, 2021, (25):3. https://biomaterialsres.biomedcentral.com/articles/

DOI: 10.1186/s40824-021-00204-y

Google Scholar

[31] Liu L, Liu J, Wang M, Min S, Cai Y, Zhu L,Yao J. Preparation and characterization of nano hydroxyapatite/silk fibroin porous scaffolds. Biomater Sci. 2012;325–338

DOI: 10.1163/156856208783721010

Google Scholar

[32] Zhao, H., Li, L., Ding, S., Liu, C., and Ai, J. Effect Of Porous Structure and Pore Size on Mechanical Strength of 3D-Printed Comby Scaffolds. Materials Letters. 2018. 223: 21–24

DOI: 10.1016/j.matlet.2018.03.205

Google Scholar

[33] Warastuti, Y., & Abbas B., Synthesis and Characterization of Hydroxyapatite-Based Irradiated Injectable Bone Substitute Paste. Journal of Isotope and Radiation Applications, 2011, 7(2), 73-82. https://adoc.pub/queue/synthesis-and-characterization-of-irradiated-injectable-bone.html

Google Scholar

[34] Kurutz, M., M. Galos, P. Varga, B. Fornet, Regional Age-And Sex- Related Compressive Strength Parameters of Human Lumbar Vertebrae in Osteoporosis, Portugal: 8th Computer Methods In Biomechanics And Biomedical Engineering Symposium. 2018 https://doi.org/10.2147%2Fjmdh.s4103

DOI: 10.2147/jmdh.s4103

Google Scholar

[35] Wardhani, I.F., Samudra, R.M.R., Katherine., Hikmawati, D., In vitro study of Nano Hydroxyapatite/Streptomycin-Gelatin-Based Injectable Bone Substitute Associated- 3D printed Bone Scaffold for Spinal Tuberculosis Case. Journal of Physics: Conference Series. 2020, https://iopscience.iop.org/article/

DOI: 10.1088/1742-6596/1445/1/012003

Google Scholar

[36] J. Dong, S. Zhang, J. Ma., Preparation, characterization, and in vitro cytotoxicity evaluation of a novel anti-tuberculosis reconstruction implant, PLoS ONE, vol. 9, no. 4, Article ID e94937, p.1–10, 2014

DOI: 10.1371/journal.pone.0094937

Google Scholar