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HomeJournal of Biomimetics, Biomaterials and...Journal of Biomimetics, Biomaterials and...Herbally Painted Biofunctional Scaffolds with...

Herbally Painted Biofunctional Scaffolds with Improved Osteoinductivity for Bone Tissue Engineering

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Abstract:

In the bone tissue engineering composite scaffolds with osteogenic potential are emerging as the new tool. Here, we investigated the graphene (GP), graphene oxide (GO) and Cissus quadrangularis (CQ) callus extract for their spontaneous osteoinductive potential. Electrospun poly ε-caprolactone (PCL) sheets were painted with varying combination GP, GO and CQ solutions as ink. The prepared PCL-GO, PCL-GO-CQ, PCL-GP and PCL-GP-CQ scaffolds were characterized for their physical, mechanical and biological properties. Addition of GO, GP, GO-CQ and GP-CQ to PCL enhanced roughness, wettability, Yield strength and tensile strength, biocompatibility .significantly. Presence of GO and CQ in PCL-GO-CQ scaffolds, while GP and CQ in PCL-GP-CQ scaffolds showed synergistic effect on the biocompatibility, Cell attachment,cell proliferation of human umbilical Wharton’s jelly derived mesenchymal stem cells (hUCMSCs) and their differentiation into osteoblasts by 21^st day in culture without osteogenic differentiation media or any growth factors. Same is confirmed by the Alizarin red S staining and Von kossa staining. The combination of PCL-GO-CQ scaffold prepared by novel paint method was found to be the most potential in bone tissue engineering.

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Journal of Biomimetics, Biomaterials and Biomedical Engineering Vol. 41

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Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 41)

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49-68

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https://doi.org/10.4028/www.scientific.net/JBBBE.41.49

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April 2019

Authors:

Shivaji Kashte, Gajanan Arbade, R.K. Sharma, Sachin Kadam*

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Bone Tissue Engineering, Cissus quadrangularis, Graphene, Graphene Oxide, Human Umbilical Cord Wharton’s Jelly Derived Mesenchymal Stem Cells

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© 2019 Trans Tech Publications Ltd. All Rights Reserved

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