Antibacterial Effects of Calotropisgigantea (L) Extracts on Porphyromonasgingivalis

Abstract:

The Porphyromonasgingivalis is one of the dominant bacteria that causes chronic periodontitis. These bacteria can penetrate the gingiva and cause tissue damage directly or indirectly with the induction of inflammation. The growth of Porphyromonasgingivalis can be inhibited by administrating the active compounds from plants that have an antibacterial effect such as Calotropisgigantea leaves.This study aimed to determine the antibacterial potential of Bidurileaves (Calotropisgigantea) extract against the Porphyromonasgingivalis ATCC. In this study the Biduri leaves were extracted by maceration method using 70% ethanol solvent. The antibacterial potential test of 70% ethanol extract of Biduri leaves (with a concentration of 5%, 10%, 15%, 20%, 25%, 30%, and 35%) against thePorphyromonasgingivalis was carried out using dilution method with 4 times repetition on Tryptone Soy Agar (TSA) media.The results of the study were analyzed using the One Way ANOVA test showing there were significant differences in the number of Porphyromonasgingivalis ATCC 33277 colonies due to administration of 70% ethanol extract of Bidurileaves (Calotropisgigantea) with p values <0,05. The test results were continued with the Post Hoc Tamhane test to determine the significant difference of the treatment groups. Based on the results of the study it could be concluded that 70% ethanol extract of Biduri leaves (Calotropisgigantea) had antibacterial potential against Porphyromonasgingivalis ATCC 33277 starting from the smallest concentration of 5% to the highest concentration of 35%.

• Periodical: Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 48)
• Pages: 111-118
• DOI: https://doi.org/10.4028/www.scientific.net/JBBBE.48.111
• Online since: November 2020
• Authors: Zulfan M. Alibasyah*, D.S. Ningsih, Sunnati Sunnati, R. Andayani, N.P. Ranggaswuni
• Keywords: Antibakteri, Calotropis Gigantea, Microdilusi, Porphyromonas Gingivalis

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