The Antibacterial Effect of Soursop Leaf Extract on Staphylococcus aureus ATCC^® 25923^tm (In Vitro)

Abstract:

Purpose: This study was to determine the Minimal Inhibition Concentration (MIC) and Minimal Bactericidal Concentration (MBC) soursop leaf extract antibacterial activity against bacteria Staphylococcus aureus ATCC® 25923TM at a concentration of 100, 90, 80, 70, 60 and 50%. Method: This study is an experimental laboratory with design Post Test Only Control Group Design by using soursop leaf extract at a concentration of 100, 90, 80, 70, 60 and 50% respectively. Each test is repeated four times to determine the average Minimal Inhibition Concentration (MIC) and Minimal Bactericidal Concentration (MBC). Soursop leaf extract is made by the dilution method using a medium Nutrient Broth (NB) and sub-cultured on Mannitol Salt Agar (MSA). The number of colonies of Staphylococcus aureus ATCC® 25923TM are counted using a manual calculation in the media Mueller Hinton Agar (MHA). Test Kruskkal Wallis there are differences in the antibacterial effect that was significant (p <0.05), soursop leaf extract against Staphylococcus aureus ATCC® 25923TM from each treatment group. Results: The minimum inhibitory concentration was 90% and the minimum bactericidal concentration is 100% committed. The number of colonies of Staphylococcus aureus bacteria ATCC® 25923TM at 100% concentration 0 CFU/mL 90% 234.50 CFU/ml and at concentrations of 80, 70, 60 and 50% could not count the number of colonies due to > 300 CFU/mL. As for the diameter of inhibitory zone at 100% concentration 10,625 mm, 90% 8,875 mm, 6,750 mm 80% and 70%, 60%, 50% reporting no inhibition zone diameter. Conclusion: The p-value of Kruskkal Wallis is p<0,05 which shows that there is a significant difference between the inhibition of soursop leaf extract with a concentration of 100, 90, 80, 70, 60 and 50% on growth of Staphylococcus aureus ATCC® 25923TM. Thus, soursop leaf extract has antibacterial effects against the growth of Staphylococcus aureus ATCC® 25923TM.

• Periodical: Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 48)
• Pages: 119-125
• DOI: https://doi.org/10.4028/www.scientific.net/JBBBE.48.119
• Online since: November 2020
• Authors: Minasari Nasution*, Sri Amelia, Khairiyani Asri Hasibuan
• Keywords: Diameter of Inhibition Zone, MBC, MIC, Soursop Leaf Extract, Staphylococcus aureus ATCC® 25923TM

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