Paper Titles

Synthesis, Molecular Modeling, DNA Damage Interaction, and Antioxidant Potential of Hesperidin Loaded on Gold Nanoparticles
p.17

Blood Pressure Estimation from Photoplethysmography with Motion Artifacts Using Long Short Term Memory Network
p.31

Vasodilatation Measurement Using Finger Vascular Images by Near-Infrared Light and Comparison with RH-PAT
p.41

Master Frame Extraction of Fetal Cardiac Images Using B Mode Ultrasound Images
p.51

A Hybrid Machine Learning Model Based on Global and Local Learner Algorithms for Diabetes Mellitus Prediction
p.65

Comparison of Stress Distribution in Surrounding Bone during Insertion of Dental Implants on Four Implant Threads under the Effect of an Impact: A Finite Element Study
p.89

Cytotoxicity and Ion Release of Functionally Graded Al₂O₃- Ti Orthopedic Biomaterial
p.103

Heat Treatment Effect on Biological Behavior of Polyetheretherketone Composites
p.119

Synthesis and Characterization of Phosphoric Silica Catalyst from Bamboo Leaves for Production of Triacetin
p.129

HomeJournal of Biomimetics, Biomaterials and...Journal of Biomimetics, Biomaterials and...Cytotoxicity and Ion Release of Functionally...

Cytotoxicity and Ion Release of Functionally Graded Al₂O₃- Ti Orthopedic Biomaterial

Article Preview

Abstract:

The aim of this study was to evaluate the biocompatibility of Al₂O₃-Ti functionally graded material (FGM) successfully fabricated by Spark Plasma Sintering (SPS) technology, and to compare with pure Ti and alumina. Pre-osteoblast MC3T3-E1 cells were used to examine cell viability, proliferation and differentiation using lactate dehydrogenase (LDH) cytotoxicity detection kit, MTT assay and Alkaline Phosphatase (ALP) colorimetric test at different time points. Furthermore, ion release from the materials into the culture medium was assessed. The results showed cell viability over 80% for FGM and alumina which dismissed any cytotoxicity risk due to materials or manufacturing. The results of MTT tests identified superiority of FGM than Ti and alumina, particularly in late proliferation. Nevertheless, in cell differentiation, all materials performed similarly with no statistical differences. Furthermore, it was indicated that Ti had no ion release, while alumina had small amount of Al ion dissolution. FGM, however, had more ions detachment, particularly Al ions.

You might also be interested in these eBooks

Journal of Biomimetics, Biomaterials and Biomedical Engineering Vol. 54

Info:

Periodical:

Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 54)

Pages:

103-118

DOI:

https://doi.org/10.4028/www.scientific.net/JBBBE.54.103

Citation:

Cite this paper

Online since:

January 2022

Authors:

Marjan Bahraminasab*, Samaneh Arab, Nesa Doostmohammadi

Keywords:

Biological Response, In Vitro Tests, Layered Composite, Orthopedic Applications, Osteoblastic Cell Line

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2022 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] M. Bahraminasab, M.R. Hassan, and B.B. Sahari, Metallic biomaterials of knee and hip - A review. Trends Biomater Artif Organs. 24 (2010) 69-82.

[2] M. Bahraminasab and K.L. Edwards, Biocomposites for Hard Tissue Replacement and Repair, in: B.P. Sidhu S., Zitoune R., Yazdani M. (Eds), Futuristic Composites. Materials Horizons: From Nature to Nanomaterials, Springer, Singapore, 2018, pp.281-296.

DOI: 10.1007/978-981-13-2417-8_14

[3] M. Bahraminasab and F. Farahmand, State of the art review on design and manufacture of hybrid biomedical materials: Hip and knee prostheses. Proc. Inst. Mech. Eng. [H] J. Eng. Med. (2017) 1-29.

DOI: 10.1177/0954411917705911

[4] J. Liu, et al., 3D printing of biomimetic multi-layered GelMA/nHA scaffold for osteochondral defect repair. Mater. Des. 171 (2019) 1-9.

DOI: 10.1016/j.matdes.2019.107708

[5] F. Baino, Chapter 16 - Functionally Graded Bioactive Glass-Derived Scaffolds Mimicking Bone Tissue, in: G. Kaur (Eds), Biomedical, Therapeutic and Clinical Applications of Bioactive Glasses Woodhead Publishing, 2019, pp.443-466.

DOI: 10.1016/b978-0-08-102196-5.00016-1

[6] J. Pavón, et al., Development of new titanium implants with longitudinal gradient porosity by space-holder technique. J Mater Sci. 50 (2015) 6103-6112.

DOI: 10.1007/s10853-015-9163-1

[7] D. Mahmoud and M.A. Elbestawi, Lattice structures and functionally graded materials applications in additive manufacturing of orthopedic implants: a review. J. Manuf. Mater. Process. 1 (2017) 1-19.

DOI: 10.3390/jmmp1020013

[8] M.R. Ayatollahi, et al., To Improve Total Knee Prostheses Performance Using Three-phase Ceramic-based Functionally Graded Biomaterials. Front. Mater. Sci. 6 (2019) 1-9.

DOI: 10.3389/fmats.2019.00107

[9] A. Sola, D. Bellucci, and V. Cannillo, Functionally graded materials for orthopedic applications–an update on design and manufacturing. Biotechnol. Adv. 34 (2016) 504-531.

DOI: 10.1016/j.biotechadv.2015.12.013

[10] A. Oshkour, et al., Mechanical and physical behaviour of newly developed functionally graded materials and composites of stainless steel 316L with calcium silicate and hydroxyapatite. J Mech Behav Biomed Mater. 49 (2015) 321-331.

DOI: 10.1016/j.jmbbm.2015.05.020

[11] A.A. Oshkour, et al., Effect of Geometrical Parameters on the Performance of Longitudinal Functionally Graded Femoral Prostheses. Artif. Organs. 39 (2015) 156-164.

DOI: 10.1111/aor.12315

[12] G.M. Kumar. Functionally graded bio-ceramic reinforced PVA hydrogel composites for knee joint artificial cartilages. in AIP Conference Proceedings. 2018. AIP Publishing.

DOI: 10.1063/1.5029689

[13] T. Kawai, et al., Customized, degradable, functionally graded scaffold for potential treatment of early stage osteonecrosis of the femoral head. J. Orthop. Res. 36 (2018) 1002-1011.

DOI: 10.1002/jor.23673

[14] M. Monzón, et al., Functionally graded additive manufacturing to achieve functionality specifications of osteochondral scaffolds. BDM. 1 (2018) 69-75.

DOI: 10.1007/s42242-018-0003-4

[15] M. Bahraminasab, et al., Material tailoring of the femoral component in a total knee replacement to reduce the problem of aseptic loosening. Mater. Des. 52 (2013) 441-451.

DOI: 10.1016/j.matdes.2013.05.066

[16] M. Bahraminasab, et al., Multi-objective design optimization of functionally graded material for the femoral component of a total knee replacement. Mater. Des. 53 (2014) 159-173.

DOI: 10.1016/j.matdes.2013.06.050

[17] M. Bahraminasab, et al., On the influence of shape and material used for the femoral component pegs in knee prostheses for reducing the problem of aseptic loosening. Mater. Des. 55 (2014) 416-428.

DOI: 10.1016/j.matdes.2013.10.020

[18] A.A. Atiyah, S.B. Farid, and D.N. Abdulamer, Fabrication of Ceramic-Metal Functionally Graded Materials. J. Eng. Technol. 31 (2013) 513-524.

[19] M. Bahraminasab, S. Ghaffari, and H. Eslami-Shahed, Al2O3-Ti functionally graded material prepared by spark plasma sintering for orthopaedic applications. J Mech Behav Biomed Mater. 72 (2017) 82-89.

DOI: 10.1016/j.jmbbm.2017.04.024

[20] Y. Zhang and A. Bandyopadhyay, Direct fabrication of compositionally graded Ti-Al2O3 multi-material structures using Laser Engineered Net Shaping. Addit. manuf. 21 (2018) 104-111.

DOI: 10.1016/j.addma.2018.03.001

[21] T. Fujii, et al., Fracture toughness distribution of alumina-titanium functionally graded materials fabricated by spark plasma sintering. J Alloys Compd. 766 (2018) 1-11.

DOI: 10.1016/j.jallcom.2018.06.304

[22] C. Madec, et al., Alumina-titanium functionally graded composites produced by spark plasma sintering. J. Mater. Process. Technol. 254 (2018) 277-282.

DOI: 10.1016/j.jmatprotec.2017.11.004

[23] C.F. Gutierrez-Gonzalez, et al., Processing, spark plasma sintering, and mechanical behavior of alumina/titanium composites. J Mater Sci. 49 (2014 ) 3823-3830.

DOI: 10.1007/s10853-014-8095-5

[24] S. Hayun, et al., Phase Constitution and Dynamic Properties of Spark Plasma‐Sintered Alumina–Titanium Composites. J. Am. Ceram. Soc. 99 (2015) 573-580.

DOI: 10.1111/jace.13992

[25] S. Meir, et al., Mechanical properties of Al 2 O 3\ Ti composites fabricated by spark plasma sintering. Ceram Int. 41 (2015 ) 4637-4643.

DOI: 10.1016/j.ceramint.2014.12.008

[26] T. Fujii, et al., Fabrication of alumina-titanium composites by spark plasma sintering and their mechanical properties. J Alloys Compd. 744 (2018 ) 759-768.

DOI: 10.1016/j.jallcom.2018.02.142

[27] M. Bahraminasab, et al., Corrosion of Al2O3-Ti composites under inflammatory condition in simulated physiological solution. Mater Sci Eng C Mater Biol Appl. 102 (2019 ) 200-211.

DOI: 10.1016/j.msec.2019.04.047

[28] M. Bahraminasab, et al., Electrochemical corrosion of Ti-Al2O3 biocomposites in Ringer's solution. J Alloys Compd. 777 (2019 ) 34-43.

DOI: 10.1016/j.jallcom.2018.09.313

[29] M. Bahraminasab, et al., In vivo performance of Al 2 O 3-Ti bone implants in the rat femur. J. Orthop. Surg. Res. 16 (2021) 1-14.

[30] T. Fujii, et al., Fabrication and strength evaluation of biocompatible ceramic-metal composite materials. J. Solid Mech. Mater. Eng. 4 (2010) 1699-1710.

[31] T. Fujii, et al., Fabrication of a PSZ-Ti functionally graded material by spark plasma sintering and its fracture toughness. Mat. Sci Eng., A. 682 (2017) 656-663.

DOI: 10.1016/j.msea.2016.11.091

[32] E. Fernandez-Garcia, et al., Osteoblastic cell response to spark plasma-sintered zirconia/titanium cermets. J Biomater Appl. 29 (2014) 813-823.

DOI: 10.1177/0885328214547400

[33] R. Guzman, et al., Biocompatibility assessment of spark plasma-sintered alumina-titanium cermets. J Biomater Appl. 30 (2016) 759-769.

DOI: 10.1177/0885328215584858

[34] B.-D. Hahn, et al., Mechanical and in vitro biological performances of hydroxyapatite–carbon nanotube composite coatings deposited on Ti by aerosol deposition. Acta Biomater. 5 (2009) 3205-3214.

DOI: 10.1016/j.actbio.2009.05.005

[35] R.E. McMahon, et al., A comparative study of the cytotoxicity and corrosion resistance of nickel–titanium and titanium–niobium shape memory alloys. Acta Biomater. 8 (2012) 2863-2870.

DOI: 10.1016/j.actbio.2012.03.034

[36] M. Li, et al., Cytotoxic Effect on Osteosarcoma MG-63 Cells by Degradation of Magnesium. J. Mater. Sci. Technol. 30 (2014) 888-893.

[37] A. Benko, et al., Titanium Surface Modification with Carbon Nanotubes. Towards Improved Biocompatibility. Acta Phys. Pol. 129 (2016) 176-178.

DOI: 10.12693/aphyspola.129.176

[38] L. Braz, et al., Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization. Int. J. Biol. Macromol. 96 (2017) 786-797.

DOI: 10.1016/j.ijbiomac.2016.12.076

[39] S. Spriano, et al., How do wettability, zeta potential and hydroxylation degree affect the biological response of biomaterials? Mater Sci Eng C Mater Biol Appl. 74 (2017) 542-555.

DOI: 10.1016/j.msec.2016.12.107

[40] S. Chen, et al., Tuning surface properties of bone biomaterials to manipulate osteoblastic cell adhesion and the signaling pathways for the enhancement of early osseointegration. Colloids Surf B Biointerfaces. 164 (2018) 58-69.

DOI: 10.1016/j.colsurfb.2018.01.022

[41] K. Rabel, et al., Controlling osteoblast morphology and proliferation via surface micro-topographies of implant biomaterials. Sci Rep. 10 (2020) 1-14.

DOI: 10.1038/s41598-020-69685-6

[42] H.-C. Ko, et al., Initial osteoblast-like cell response to pure titanium and zirconia/alumina ceramics. Dent. Mater. 23 (2007) 1349-1355.

DOI: 10.1016/j.dental.2006.11.023

[43] E. Saberi, et al., Proliferation, odontogenic/osteogenic differentiation, and cytokine production by human stem cells of the apical papilla induced by biomaterials: A comparative study. Clin. Cosmet. Investig. Dent. 11 (2019) 181-193.

DOI: 10.2147/ccide.s211893

[44] K. Anselme, Osteoblast adhesion on biomaterials. Biomaterials. 21 (2000) 667-681.

DOI: 10.1016/s0142-9612(99)00242-2

[45] Q. Huang, et al., Enhanced SaOS-2 cell adhesion, proliferation and differentiation on Mg-incorporated micro/nano-topographical TiO2 coatings. Appl. Surf. Sci. 447 (2018) 767-776.

DOI: 10.1016/j.apsusc.2018.04.095

[46] F. Robotti, et al., A micron-scale surface topography design reducing cell adhesion to implanted materials. Sci Rep. 8 (2018) 1-13.

DOI: 10.1038/s41598-018-29167-2

[47] K. Oya, et al., Calcification by MC3T3-E1 cells on RGD peptide immobilized on titanium through electrodeposited PEG. Biomaterials. 30 (2009) 1281-1286.

DOI: 10.1016/j.biomaterials.2008.11.030

[48] D.-H. Lee, et al., MC3T3-E1 cell response to pure titanium, zirconia and nano-hydroxyapatite. Int. J. Mod. Phys. B. 23 (2009) 1535-1540.

DOI: 10.1142/s0217979209061226

[49] F. He, et al., Enhanced initial proliferation and differentiation of MC3T3-E1 cells on HF/HNO3 solution treated nanostructural titanium surface. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 110 (2010) 13-22.

DOI: 10.1016/j.tripleo.2010.03.044

[50] B. Zhang, et al., Surface characterization and cell response of binary Ti-Ag alloys with CP Ti as material control. J. Mater. Sci. Technol. 28 (2012) 779-784.

DOI: 10.1016/s1005-0302(12)60130-3

[51] J.-J. Lee, et al., Evaluation of effect of galvanic corrosion between nickel-chromium metal and titanium on ion release and cell toxicity. J Adv Prosthodont. 7 (2015) 172-177.

DOI: 10.4047/jap.2015.7.2.172

[52] S. Höhn and S. Virtanen, Effect of inflammatory conditions and H2O2 on bare and coated Ti–6Al–4V surfaces: Corrosion behavior, metal ion release and Ca-P formation under long-term immersion in DMEM. Appl. Surf. Sci. 357 (2015) 101-111.

DOI: 10.1016/j.apsusc.2015.08.261

[53] R. Forrer, K. Gautschi, and H. Lutz, Simultaneous measurement of the trace elements Al, As, B, Be, Cd, Co, Cu, Fe, Li, Mn, Mo, Ni, Rb, Se, Sr, and Zn in human serum and their reference ranges by ICP-MS. Biol. Trace Elem. Res. 80 (2001) 77-93.

DOI: 10.1385/bter:80:1:77

[54] N.J. Hallab, et al., Orthopaedic implant related metal toxicity in terms of human lymphocyte reactivity to metal-protein complexes produced from cobalt-base and titanium-base implant alloy degradation. Mol. Cell. Biochem. 222 (2001) 127-136.

DOI: 10.1007/978-1-4615-0793-2_15

[55] A. Sargeant and T. Goswami, Hip implants - Paper VI - Ion concentrations. Mater. Des. 28 (2007) 155-171.

DOI: 10.1016/j.matdes.2005.05.018

[56] L. Balcaen, et al., Accurate determination of ultra-trace levels of Ti in blood serum using ICP-MS/MS. Anal. Chim. Acta. 809 (2014) 1-8.

DOI: 10.1016/j.aca.2013.10.017

[57] L. Rodella, et al., Aluminium exposure induces Alzheimer s disease-like histopathological alterations in mouse brain. Histol. Histopathol. 23 (2008) 433-439.

[58] C. Exley, The toxicity of aluminium in humans. Morphologie. 100 (2016) 51-55.

[59] S. Yoganathan, et al., Prevalence and predictors of peripheral neuropathy in nondiabetic children with chronic kidney disease. Muscle Nerve. 57 (2018) 792-798.

DOI: 10.1002/mus.26027

[60] J. Michl, K.C. Park, and P. Swietach, Evidence-based guidelines for controlling pH in mammalian live-cell culture systems. Commun. Biol. 2 (2019) 1-12.

DOI: 10.1038/s42003-019-0393-7