Kinetics of Essential Oil Extraction of Kaffir Lime Peel Using Microwave Assisted Hydrodistillation

Abstract:

The purpose of this study is to study the kinetics of kaffir lime peel essential oil extraction using microwave assisted hydrodistillation. Extraction was carried out at various powers and the ratios of material to solvent for 1 hour. Soxhlet extraction using n-hexane solvent was also carried out to determine the yield of essential oil. The extraction kinetics was investigated using a mass transfer-controlled approach and expressed by the 1st and 2nd order of reaction rate. The Soxhlet extraction results indicated that the yield of kaffir lime peel essential oil was 5.65% w/w. The higher the microwave power being applied (180, 300, 450, and 600 Watt), the higher the essential oil yielded (2.2; 2.8; 3; and 3.8% w/w), respectively, but at 800 Watt, the yield decreased (3.2% w/w). The smaller the ratio of material to solvent, which means more diluted, the lower the yield of oil were, namely from 4.56; 3.9; and 3.8% w/w, at ratios of material to solvent 1:3, 1:3.5, and 1:4 w/v. Different results were obtained at a ratio of 1:3 (4.56% w/w) which produced a yield greater than the ratio of 1:1.25 (2.88% w/w). Therefore, the optimum conditions for extracting kaffir lime peel essential oil were at 600 Watt and a ratio of material to solvent 1:3 w/w for 56 minutes with a yield of 4.58% and a density of 0.86 g/cm³. The kinetics of the 2nd order of homogeneous model better represented results of the experiments with extraction rate constants at 180, 300, 450, 600, and 800 Watt of 14.89; 14.95; 15.53; 21.32; and 19.85 L.g^-1.min^-1. While the extraction rate constants at the material to solvent ratio of 1:2.5; 1:3; 1:3.5; and 1:4 w/v of 17.38; 19.80; 22.09; and 32.31 L.g^-1.min^-1. The extraction capacity was also affected by the power and the ratio of material to solvent, the extraction capacity were 0.0095; 0.0100; 0.0104; 0.0125; and 0.0106 g.L^-1, at 180, 300, 450, 600, and 800 Watt respectively, and 0.0094; 0.0134; 0.0134; and 0.0118 g.L^-1, at material to solvent ratio of 1:2.5; 1:3; 1:3,5; and 1:4 w/v respectively.

• Periodical: Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 63)
• Pages: 129-140
• DOI: https://doi.org/10.4028/p-W3TmCa
• Online since: November 2023
• Authors: Megawati Megawati*, Bayu Triwibowo, Zuhriyan Ash Shiddieqy Bahlawan, Junaidah Jai, Pradipta Sadewa, Yuni Parastuti
• Keywords: Essential Oil, Kaffir Lime Peel, Kinetics, Mass Transfer-Controlled Rate, Microwave Assisted Hydrodistillation

References

[1] M. Lubinska-Szczygeł, A. Różańska, T. Dymerski, J. Namieśnik, E. Katrich, & S. Gorinstein. A novel analytical approach in the assessment of unprocessed Kaffir lime peel and pulp as potential raw materials for cosmetic applications. Industrial Crops and Products 120 (2018) 313–321.

DOI: 10.1016/j.indcrop.2018.04.036

[2] M.M. Mya, Z.Z. Aung, C.T. Nwe, A.W. Oo, T.M. Htay, S. Thaung, & Y.N.M. Maung. Larvicidal, Ovicidal and repellent effect of Citrus hystrix DC (Kaffir lime) fruit, peel and internal materials extracts on Aedes aegypti mosquitoes. Journal of Biological Engineering Research and Review 4 (2017) 34–43.

[3] A. Sreepian, P.M. Sreepian, C. Chanthong, T. Mingkhwancheep, & P. Prathit. Antibacterial activity of essential oil extracted from Citrus hystrix (Kaffir lime) peels: An in vitro study. Tropical Biomedicine 36 (2019) 531–541.

DOI: 10.1016/j.jsps.2023.03.020

[4] M. de J. Rostro-Alanis, J. Báez-González, C. Torres-Alvarez, R. Parra-Saldívar, J. Rodriguez-Rodriguez, & S. Castillo. Chemical composition and biological activities of oregano essential oil and its fractions obtained by vacuum distillation. Molecules 24 (2018) 1-15.

DOI: 10.3390/molecules24101904

[5] N.K. Erliyanti, E.A. Saputro, R.R. Yogaswara, & E. Rosyidah. Aplikasi Metode Microwave Hydrodistillation pada Ekstraksi Minyak Atsiri dari Bunga Kamboja (Plumeria alba). Jurnal IPTEK 24 (2020) 37–44.

DOI: 10.31284/j.iptek.2020.v24i1.865

[6] M.N.C. Isa, A. Ajit, A. Naila, A.Z. Sulaiman. Effect of microwave assisted hydrodistillation extraction on extracts of Ficus deltoidei, Materials Today: Proceedings 5 (2018) 21772–21779.

DOI: 10.1016/j.matpr.2018.07.031

[7] Megawati, D.S. Fardhyanti, W.B. Sediawan, & A. Hisyam. Kinetics of mace (Myristicae arillus) essential oil extraction using microwave assisted hydrodistillation: Effect of microwave power. Industrial Crops and Products 131 (2019) 315–322.

DOI: 10.1016/j.indcrop.2019.01.067

[8] T.C.Q. Ngo, T.K.N. Tran, V.M. Nguyen, & H.C. Mai. Optimization of green mandarin (Citrus reticulata) essential oil extraction using microwave-assisted hydrodistillation and chemical composition analysis. IOP Conference Series: Materials Science and Engineering 991 (2020).

DOI: 10.1088/1757-899x/991/1/012122

[9] R.L. Monteiro, A.I. Gomide, J.V. Link, B.A.M. Carciofi, & J.B. Laurindo. Microwave vacuum drying of foods with temperature control by power modulation. Innovative Food Science and Emerging Technologies 65 (2020) 102473.

DOI: 10.1016/j.ifset.2020.102473

[10] Megawati & F. Murniyawati. Microwave Assisted Hydrodistillation Untuk Ekstraksi Minyak Atsiri Dari Kulit Jeruk Bali Sebagai Lilin Aromaterapi. Jurnal Bahan Alam Terbarukan 4 (2015) 14–20.

DOI: 10.24853/konversi.5.1.25-30

[11] Megawati, R.R. Ginting, R.D. Kusumaningtyas, & W.B. Sediawan. Mangosteen Peel Antioxidant Extraction and Its Use to Improve the Stability of Biodiesel B20 Oxidation, in Z.A. Zakaria, C.N. Aguilar, R.D. Kusumaningtyas, P. Binod (Eds.), Valorisation of Agro-industrial Residues – Volume II: Non-Biological Approaches, Springer, Switzerland, 2020, pp.29-61.

DOI: 10.1007/978-3-030-39208-6_2

[12] N.P. Putri, A.P.S. Jurin, & S.A. Ganna. Pemodelan Transfer Massa Tannin Pada Tanaman Putri Malu. JIP 5 (2015) 115–119.

[13] F.P. Kristianto, S. Machmudah, S. Winardi, Wahyudiono & M. Goto. Yield and Extraction Rate Analysis of Phytochemical Compounds from Eucheuma cottonii, Ganoderma lucidum, and Gracilaria sp. using Subcritical Water Extraction. ASEAN Journal of Chemical Engineering 21 (2021) 27–37.

DOI: 10.22146/ajche.60513

[14] H.S. Kusuma & M. Mahfud. Kinetic studies on extraction of essential oil from sandalwood (Santalum album) by microwave air-hydrodistillation method. Alexandria Engineering Journal 57 (2017) 1163–1172.

DOI: 10.1016/j.aej.2017.02.007

[15] T.I. Rohadi, H.S. Kusuma, E.F. Daniswara, A. Altway & Mahfud. Preliminary study: Comparison of kinetic models of oil extraction from vetiver (Vetiveria zizanioides) by microwave hydrodistillation. Korean Chemical Engineering Research 55 (2017) 574–577.

DOI: 10.1088/1755-1315/101/1/012015

[16] Shintawati, Analianasari, & Zukryandry. Kinetika Ekstraksi Minyak Atsiri Lada Hitam (Piper nigrum) Secara Hidrodistilasi. CHEESA: Chemical Engineering Research Articles 3 (2020) 63–70.

DOI: 10.25273/cheesa.v3i2.7388.63-70

[17] T. Syarifah. Ekstraksi Minyak Atsiri dari Batang, Daun dan Kulit Jeruk Purut (Citrus hystrix) Dengan Metode Solvent-Free Microwave Extraction. In Skripsi. Institut Teknologi Sepuluh Nopember, 2017.

DOI: 10.31942/inteka.v5i1.3394

[18] R.C. Yuda, Irdiansyah & I. Prihatiningtyas. Studi Kinetika Pengaruh Suhu terhadap Ekstraksi Minyak Atsiri dari Kulit Jeruk Nipis dengan Pelarut Etanol. Jurnal Chemurgy 01(2017)22–26.

DOI: 10.30872/cmg.v1i1.1135

[19] Q. Zhang, R. Huo, Y. Ma, S. Yan, L. Yang & F. Chen. A novel microwave-assisted steam distillation approach for separation of essential oil from tree peony (Paeonia suffruticosa Andrews) petals: Optimization, kinetic, chemical composition and antioxidant activity. Industrial Crops and Products 154 (2020) 112669.

DOI: 10.1016/j.indcrop.2020.112669

[20] S. Chotikamas, K. Cheenkachorn, B. Wongpanit, P. Tantayotai & M. Sriariyanun. Chemical Profiling Analysis and Identification the Bioactivities of Herbal Compress Extracts. MATEC Web of Conferences 187 (2018).

DOI: 10.1051/matecconf/201818701001

[21] TsegayeFekadu, T. Seifu & A. Abera. Extraction of Essential Oil from Orange Peel using Different Methods and Effect of Solvents, Time, Temperature to Maximize Yield. International Journal of Engineering Science and Computing 9 (2019) 24300–24308.

[22] M. Dobrzyńska-Mizera, M. Knitter, S. Mallardo, M.C. Del Barone, G. Santagata & M.L. Di Lorenzo. Thermal and Thermo-Mechanical Properties of Poly (L-Lactic Acid) Biocomposites Containing β-Cyclodextrin/d-Limonene Inclusion Complex. Materials 14 (2021) 2569.

DOI: 10.3390/ma14102569

[23] P.A. Handayani, N.S. Ramadani & D. Kartika. Pemungutan Tanin Propagul Mangrove (Rhizopora mucronata) dengan Pelarut Etanol dan Aquades Sebagai Zat Warna Alami Menggunakan Metode Microwave Assisted Extraction. Jurnal Kompetensi Teknik 10 (2018) 22–27.

DOI: 10.36526/jc.v2i1.922

[24] J. Bustamante, S. van Stempvoort, M. García-Gallarreta, J.A. Houghton, H.K. Briers, V.L. Budarin, A.S. Matharu & J.H. Clark. Microwave assisted hydro-distillation of essential oils from wet citrus peel waste. Journal of Cleaner Production 137 (2016) 598–605.

DOI: 10.1016/j.jclepro.2016.07.108

[25] E. Husni, S. Utari & D. Putri. Chemical Content Profile of Essential Oil from Kaffir Lime (Citrus hystrix DC.) in Tanah Datar Regency and Antibacterial Activity. Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 40 (2021) 174–181.

DOI: 10.2991/ahsr.k.211105.025

[26] A. Di Girolamo, J. Durairaj, A. van Houwelingen, F. Verstappen, D. Bosch, K. Cankar, H. Bouwmeester, D. de Ridder, A.D.J. van Dijk & J. Beekwilder. The santalene synthase from Cinnamomum camphora: Reconstruction of a sesquiterpene synthase from a monoterpene synthase. Archives of Biochemistry and Biophysics 695 (2020) 108647.

DOI: 10.1016/j.abb.2020.108647

[27] M. Arfania. Telaah Fitokimia Ekstrak Etanol Daun Jeruk Purut (Citrus Hystrix Dc) di Kabupaten Karawang. PharmaXplore Jurnal Ilmu Farmasi 2 (2017) 131–135.

DOI: 10.36805/farmasi.v2i2.323

[28] N.R. Aniyati, Y. Lukmayani & E.R. Sadiyah. Kajian Pustaka Potensi Aktivitas Antibakteri Minyak Atsiri Daun Nilam (Pogostemon cablin Benth.) terhadap Bakteri Patogen pada Kulit. Bandung Conference Series: Pharmacy 2 (2022) 1-4.

DOI: 10.29313/bcsp.v2i2.4406

[29] M.A. Ferhat, M.N. Boukhatem, M. Hazzit, B.Y. Meklati & F. Chemat. Cold Pressing, Hydrodistillation and Microwave Dry Distillation of Citrus Essential Oil from Algeria: A Comparative Study. Electronic Journal of Biology S1 (2016) 30–41.

DOI: 10.1002/ffj.1829