Comparison of Recombinant Growth/Differentiation Factor-5 (rGDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic

Abstract:

Article Preview

Various recombinant growth factors have been used for promoting osteoblastic differentiation cascade. To compare the growth/differentiation factor-5 (GDF-5) and bone morphogenetic protein-2 (BMP-2) in the in vivo osteogenic potential of bone marrow mesenchymal stem cells (MSCs), the bone formation was assessed by rat subcutaneous implantation of 5 kinds of hydroxyapatite (HA) implants; namely GDF/HA composites, BMP/HA composites, MSCs/HA composites and the MSCs/HA composites supplemented with recombinant mouse GDF-5 (GDF/MSCs/HA) or recombinant human BMP-2 (BMP/MSCs/HA). Neither the GDF/HA nor the BMP/HA composites exhibited any bone formation at any time after implantation. At both 2 and 4 weeks after implantation, obvious de novo bone formation together with active osteoblasts was seen histologically in many pores of the GDF/MSCs/HA and BMP/MSCs/HA composites. The GDF/MSCs/HA and BMP/MSCs/HA composites also showed high alkaline phosphatase (ALP) and osteocalcin expression determined at both the protein and gene levels. Compared with GDF/MSCs/HA, the BMP/MSCs/HA composites exhibited excellent osteogenesis with relatively early osteoblastic phenotype expression. These findings indicate that the two growth factors synergistically enhance de novo bone formation capability of MSCs/HA composites and the importance of ceramic surface to retain and to deliver the molecules of growth factors for the cell differentiation and maturation.

Info:

Periodical:

Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.

Pages:

945-948

DOI:

10.4028/www.scientific.net/KEM.284-286.945

Citation:

H. Shimaoka et al., "Comparison of Recombinant Growth/Differentiation Factor-5 (rGDF-5) and Bone Morphogenetic Protein-2 (rBMP-2) in the In Vivo Bone Formation in Porous Hydroxyapatite Ceramic ", Key Engineering Materials, Vols. 284-286, pp. 945-948, 2005

Online since:

April 2005

Export:

Price:

$38.00

[1] H. Ohgushi, Y. Dohi, S. Tamai and S. Tabata: J. Biomed. Mater. Res. 27 (1993) 1401-1407.

[2] T. Yoshikawa, H. Ohgushi, T. Uemura, H. Nakajima, K. Ichijima, S. Tamai and T. Tateishi: Biomed. Mater. Eng. 8 (5-6) (1998) 311-320.

[3] T. Noshi, T. Yoshikawa, Y. Dohi, M. Ikeuchi, K. Horiuchi, K. Ichijima, M. Sugimura, K. Yonemasu and H. Ohgushi: Artif. Organs 25 (2001) 201-208.

DOI: 10.1046/j.1525-1594.2001.025003201.x

[4] M. Ikeuchi, Y. Dohi, K. Horiuchi, H. Ohgushi, T. Noshi, T. Yoshikawa, K. Yamamoto and M. Sugimura:J. Biomed. Mater. Res. 60 (2002) 61-69.

DOI: 10.1002/jbm.1281

[5] H. Shimaoka, Y. Dohi, H. Ohgushi, M. Ikeuchi, M. Okamoto, A. Kudo, T. Kirita and K. Yonemasu: J. Biomed. Mater. Res. 68A (2004).

DOI: 10.1002/jbm.a.20059

In order to see related information, you need to Login.