Immobilization of Albumin and Heparin on the Surface of Ti-O Films for Improving Blood Compatibility

Feng Juan Jing; Nan Huang; Jun Ying Chen; Yong Xiang Leng; H. Sun

doi:10.4028/www.scientific.net/KEM.288-289.303

Paper Titles

Influence of Characteristics of Titanium Oxide Film on the Biological Behavior of Cultured Human Umbilical Vein Endothelial Cells (Huvec)
p.287

Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films
p.291

Surface Modification for Controlling the Blood-Materials Interface
p.295

Structure and Hemocompatibility of Titanium Oxide Films Synthesized by Continuous or Pulsed DC Magnetron Sputtering
p.299

Immobilization of Albumin and Heparin on the Surface of Ti-O Films for Improving Blood Compatibility
p.303

Iridium Oxide as a Stimulating Neural Electrode Formed by Reactive Magnetron Sputtering
p.307

The Role of Surface Physical Properties on Blood Compatibility of Titanium Oxide Films Synthesized by Unbalanced Magnetron Sputtering
p.311

Surface Modification of Titanium Implant and In Vitro Biocompatibility Evaluation
p.315

Bioactivity of Plasma-Sprayed Diopside Coating In Vitro
p.319

HomeKey Engineering MaterialsKey Engineering Materials Vols. 288-289Immobilization of Albumin and Heparin on the...

Immobilization of Albumin and Heparin on the Surface of Ti-O Films for Improving Blood Compatibility

Abstract:

Aminopropyltriethoxysilane (APTE) was covalently interacted with hydroxyl on the surface of Ti-O films prepared by magnetron sputtering, and albumin and heparin were immobilized on the APTE-coated surface with addition of 1-ethyl-3- (3-dimethyl aminopropyl) carbodiimide (EDC) as cross-link agent. X-ray photoelectron spectroscopy (XPS) was used to investigate the modified surface. Contact angle results indicate that there was an increase in the contact angle to the surface of APTE-coated Ti-O films, and a decrease in the contact angle to the surface of albuminand heparin-immobilized surfaces. Blood platelet adhesion in vitro was improved significantly after immobilization of albumin and heparin on Ti-O films. Endothelial cell (EC) culture tests showed that EC could grow on the surface of Ti-O films immobilized with albumin and heparin, but the growth and proliferation behavior of endothelial cells was not as good as on naked Ti-O films. This investigation showed that the surface of bio-inert inorganic materials immobilized with biological molecules is feasible and effective for improving the blood compatibility.