Osteogenic Differentiation of Marrow Mesenchymal Stem Cells (MSCs) on Ceramic Total Joints - Significance of Patient's Serum for Proliferation and Differentiation of MSCs -

Abstract:

After culture expansion of mesenchymal stem cells (MSCs) from a few milliliter of fresh patient’s bone marrow, we applied the MSCs on alumina ceramic ankle prosthesis and further cultured in an osteogenic medium for 2 weeks. After the culture, the MSCs differentiated into osteoblasts, which fabricated bone matrix on the surface of ceramic prosthesis. The expansion of MSCs followed by osteogenic differentiation was done using the commercially available medium with some chemicals and patient’s own serum. The MSCs well proliferated and differentiated into osteoblasts, even the MSCs were from old aged (more than 70 years old) patients. The tissue engineered ceramic prostheses were implanted into osteoarthritic patients. Typical X-ray findings showed that radiodense areas began to appear around the cell-seeded areas on the prosthesis about 2 to 3 months after the operation. These findings confirmed the importance of tissue engineering approach for early bone fixation and the approach can be done using small number of bone marrow cells and patient’s own serum without adding animal-derived products.

• Periodical: Key Engineering Materials (Volumes 309-311)
• Pages: 1383-1386
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.309-311.1383
• Online since: May 2006
• Authors: Hajime Ohgushi, Hiroko Machida, Akira Oshima, Noriko Kotobuki, Motohiro Hirose, Akira Fujisawa, Yasuhito Tanaka, Yoshinori Takakura
• Keywords: Alumina Ceramic Joint, Bone Marrow, Mesenchymal Stem Cell (MSC), Tissue Engineering

References

[1] Chanley, J. J Bone Joint Surg Br 52(2), 340-353 (1970).

[2] Kim, H.Y. & Yasuda, H.K. J Biomed Mater Res. 48(2), 135-142 (1999).

[3] Takakura Y, Tanaka Y, Sugimoto K, et. al. Clin Orthop 252: 209-16 (1990).

[4] Takaoka T, Okumura M, Ohgushi H, et. al. Biomaterials. 17: 1499-505 (1996).

[5] Hamadouche M, Witvoet J, Porcher R, et. al J Bone Joint Surg Br 83: 979-87 (2001).

[6] Ohgushi H, et. al. Biomaterials 26: 4654-61 (2005).

[7] Caplan AI, Clin Plast Surg. 21(3): 429-35 (1994).

[8] Ohgushi H, Caplan AI. J Biomed Mat Res 48: 913-27(1999).

[9] Campagnoli, C. et al. Blood. 98(8), 2396-402 (2001).

[10] Kotobuki N, Hirose M, Takakura Y, Ohgushi H. Artif Organs; 28: 33-39 (2004).

[11] Ohgushi H, Dohi Y, Katuda T, Tamai S, et. al. J Biomed Mat Res 32: 333-40 (1996).

[12] Kitamura S, Ohgushi H, Hirose M, et. al. Artif Organs. 28(1): 72-82 (2004).