Biological sealing is a key factor for successful development of percutaneous device (PD). A new device with arc-perforated flange as subcutaneous part and groove-shaped percutaneous part, was intended to improve integration of soft tissue and implant. Material and surface properties are known to have great impact on tissue-implant integration. To understand how a material and its surface property can influence tissue reaction, and to find the appropriate material for PD fabrication, five different kinds of materials were prepared for in vivo animal tests with corresponding histological evaluation. Results revealed that a more stable junction was formed between the soft tissue and HA coated titanium implant than other combinations.