Osteogenic Differentiation of Human Bone Marrow Stromal Cells Cultured on Hydroxyl Carbonate Apatite Layer Preformed on Poly (ε-aprolactone)–Organosiloxane Nano-Hybrid Materials
Previous study showed that the novel poly(ε-caprolactone)–organosiloxane nano-hybrid material (SiOPCL) had bioactivity, biodegradability, and mechanical properties comparable to those of human cancellous bone. In this study, hydroxyl carbonate apatite layer (HCA) was preformed on the surface of SiOPCL, which mimicked the events likely to occur in vivo, and cellular behaviors of human bone marrow stromal cells (hBMSCs) were investigated during the osteogenic differentiation on its surface (SiOPCL/HCA). Initial attachment, proliferation, and ALP activities of hBMSCs were comparable to those on tissue culture plates (TCPs), while the calcium content in the cell layer showed significantly higher value. It means that this novel bioactive nano-hybrid material is likely to be a promising candidate for bone grafting materials because of good hBMSCs responses as well as apatite forming ability in the simulated body fluid.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
J. J. Yoo et al., "Osteogenic Differentiation of Human Bone Marrow Stromal Cells Cultured on Hydroxyl Carbonate Apatite Layer Preformed on Poly (ε-aprolactone)–Organosiloxane Nano-Hybrid Materials ", Key Engineering Materials, Vols. 309-311, pp. 573-576, 2006