A Novel Collagen Sponge for Cartilage Tissue Engineering

Guo Ping Chen; Daisuke Akahane; Naoki Kawazoe; Yoshio Shirasaki; M. Tanaka; Katsuyuki Yamamoto; Tetsuya Tateishi

doi:10.4028/www.scientific.net/KEM.330-332.1101

Paper Titles

Effect Of HA/TCP Biphasic Ceramic on Osteoinduction
p.1083

Gene Expression Profile of Osteoblast-Like Cells on Calcium Phosphate Biomaterials
p.1087

In Vivo Bone Tissue Formation Induced by Caclium Phosphate Paste Composite with Demineralized Bone Matrix
p.1091

OPG and Rankl Expression in Osteoblasts Grown on Different HA Ceramics
p.1095

A Novel Collagen Sponge for Cartilage Tissue Engineering
p.1101

Effect of a Single Period of Mechanical Strain on Gene Expression Patterns of Ets1 and Cbfa1 in Murine Calvarial Sutural Osteoblast-Like Cells
p.1105

Ectopic Osteogenesis of Bone Marrow Stromal Cells Loading in Hydroxyapatite/ β-Tricalcium Phosphate
p.1109

The Optimized Design of a Double-Layer Flow Chamber
p.1113

Calcium Phosphate Hybridized with Human Semitendinosus and Gracilis Tendon Grafts
p.1117

HomeKey Engineering MaterialsKey Engineering Materials Vols. 330-332A Novel Collagen Sponge for Cartilage Tissue...

A Novel Collagen Sponge for Cartilage Tissue Engineering

Abstract:

A novel collagen sponge that can protect cell leakage during cell seeding was developed by wrapping all the surfaces except the upside of a collagen sponge with membrane that has pores smaller than cell. The collagen sponge was used for three-dimensional culture of human bone marrow-derived mesenchymal stem cells (MSCs). The cells adhered to the collagen, and proliferated to fill the spaces in the sponge. The cell seeding efficiency was higher than 95%. The MSCs cultured in the collagen sponge in the chondrogenic induction medium supplemented with TGF-β3 and BMP6 expressed genes encoding type II collagen, SOX9 and aggrecan. HE staining indicated the round morphology of differentiated cells and the extracelluler matrices were positively stained by safranin O and toluidine blue. Type II collagen and cartilage proteoglycan were detected by immunostaining with anti-type II collagen and anti-cartilage proteoglycan. These results suggest the chondrogenic differentiation of MSCs. The collagen sponge facilitated cell seeding and chondrogenic differentiation of MSCs, and will be useful for cartilage tissue engineering.