Hydrogen Peroxide Sensor Based on Horseradish Peroxide (HRP) Immobilized PANI Electrode with Au Nanoparticles

Kang Deuk Seo; Seong Dae Oh; Seong Ho Choi; Sang Hyub Oh; Jin Chun Woo; Hae Jun Park

doi:10.4028/www.scientific.net/KEM.342-343.865

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Comparison of a PGA Sheet with a Commercial ePTFE Sheet as a Staple-Line Reinforcement in Lung Surgery
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A Novel Non-Destructive Method for Measuring Elastic Moduli of Cultivated Cartilage Tissues
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The Effects of Antibiotics Blended Chitosan Membranes on the Calvarial Critical Size Defect in Sprague Dawley Rats
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Combined Inhibitory Effect of Curcuma Xanthorrhiza Extract and Xylitol on Streptococcus Mutans and Actinomyces Viscosus
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Hydrogen Peroxide Sensor Based on Horseradish Peroxide (HRP) Immobilized PANI Electrode with Au Nanoparticles
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Immobilization of the Horseradish Peroxide on the Multi-Wall Nanotube and its Application for H₂O₂ Sensing
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Hydrothermal Synthesis of Hydroxyapatite Powders Using Alkyl Ammonium Bromides as Surfactants
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Influence of Hydrothermal Processing Parameters on Phase Stability of Hydroxyapatite
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Poly(Vinyl Alcohol)/BaSO₄ Hybrid Fibers with Radiopaque Properties
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Hydrogen Peroxide Sensor Based on Horseradish Peroxide (HRP) Immobilized PANI Electrode with Au Nanoparticles

Abstract:

In order to prepare the enzyme electrode for sensing of chemical molecules, the Au was coated on the surface of PP film by sputter coater. Polyaniline (PANI) was polymerized on the surface of the coated Au film by electrochemical polymerization of aniline in order to use working electrode. Subsequently, horseradish peroxide (HRP) was immobilized on PANI electrode as follows: Method I. The HRP was immobilized on the surface of PANI electrode by using Au nanoparticle as linker (physical adsorption). Method II. The HRP was immobilized on the surface of PANI electrode by using 2-aminothiophenol and Au nanoparticle as linker (Self-assembling immobilization). Method III. HRP was directly immobilized with PANI electrode by using glutaric dialdehyde as linker (covalent bonding). The sensing efficiency of the prepared HRP-PANI electrode was also examined. The sensing efficiency of the HRP-PANI electrode for H2O2 was as following order; Method I > Method III > Method II..