Immobilization of the Horseradish Peroxide on the Multi-Wall Nanotube and its Application for H₂O₂ Sensing

Abstract:

In order to immobilize enzyme to multi-wall nanotube (MWNT), the MWNT was coated by the electrochemical polymerization of aniline. The horseradish peroxide (HRP) was immobilized on the surface of the polyaniline (PANI)-coated MWNT (PANI-MWNT film) by using gold nanoparticle (physical immobilization). The HRP was also immobilized on the surface of PANIMWNT film by using 2-aminothiophenol and Au nanoparticle (self-assembling immobilization). HRP was immobilized on the surface of PANI-MWNT electrode by using glutaric dialdehyde (covalent immobilization). The sensing efficiency of HRP-MWNT electrodes for H2O2 was evaluated by amperometric method. The sensing efficiency of HRP-MWNT electrodes for H2O2 was as following order; physical immobilization > covalent immobilization > self-assembling immobilization.

• Periodical: Key Engineering Materials (Volumes 342-343)
• Pages: 869-872
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.342-343.869
• Online since: July 2007
• Authors: Kang Deuk Seo, Seong Dae Oh, Seong Ho Choi, Sang Hyub Oh, Jin Chun Woo, Sang Ho Kim
• Keywords: Covalent Immobilization, Horseradish Peroxide, Immobilisation, Physical Immobilization, Self-Assembling

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