Lubricated reciprocating tests were conducted on an SRV tester to investigate effects of stroke and frequency on frictional and wear properties of flaky graphite cast iron used for marine cylinder liner by varying frequency and stroke under a given sliding velocity in boundary and mixed lubrication regime. It was clarified that changes in coefficient of friction were significantly dependent on stroke and frequency: in boundary lubrication, coefficient of friction at steady state showed a lower value at a combination of lower frequency and therefore larger stroke. In mixed lubrication, however, a combination of lower frequency and larger stroke produced a higher friction. In boundary lubrication where no run-in process was observed, higher frequency contributed to producing rougher surfaces resulting in higher friction. In mixed lubrication bordering on boundary lubrication higher frequency promoted run-in, enhancing film formation and lowering friction. Effects of frequency and stroke also reflected on wear, changes in separation voltage between a disk and a ball specimen, which were in accordance with changes in friction.