In Vitro Evaluation of Chelate-Setting Cements Fabricated from Silicon-Containing Apatite Powder Using Osteoblastic Cells

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In our previous study, silicon-containing hydroxyapatite (Si-HAp) powder was prepared via an aqueous precipitation reaction. The Si-HAp powders were synthesized with desired Si contents (0, 0.4, 0.8, 1.6, and 2.4 mass%) as a nominal composition. Another previous study in our group demonstrated surface-modification of HAp powder with inositol phosphate (IP6) enhanced the compressive strength of apatite cement. Thus, to fabricate the cements with higher bioactivity, the above Si-HAp powders were surface-modified with IP6 (IP6-Si-HAp). The IP6-Si-HAp cements with various Si contents were fabricated by mixing with pure water at the powder/liquid ratio of 1/0.4 [w/v]. In order to clarify biocompatibility of the IP6-Si-HAP cements in the present work, MC3T3-E1 cells as a model of osteoblast were seeded on the cement specimens. As for the numbers of cells cultured on the IP6-Si-HAp cements, the substitution of lower levels of Si into HAp lattice did not greatly influence the cell proliferation. However, the substitution of Si amount over 0.8 mass% enhanced the cell proliferation. Especially, the IP6-Si-HAp cement with the Si content of 2.4 mass% showed excellent cell proliferation among examined specimens. Therefore, to fabricate the cements with higher bioactivity, it is necessary to control the amount of Si in the IP6-Si-HAp cements. The usage of these IP6-Si-HAp cements may make it possible to fabricate the cements with higher bioactivity, compare to conventional pure HAp cements.

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Periodical:

Key Engineering Materials (Volumes 529-530)

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Edited by:

Kunio Ishikawa and Yukihide Iwamoto

Pages:

183-186

Citation:

Y. Nakashima et al., "In Vitro Evaluation of Chelate-Setting Cements Fabricated from Silicon-Containing Apatite Powder Using Osteoblastic Cells", Key Engineering Materials, Vols. 529-530, pp. 183-186, 2013

Online since:

November 2012

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$38.00

[1] I. R. Gibson, J. Huang, S. M. Best and W. Bonfield, Enhanced in vitro cell activity and surface apatite layer formation on novel silicon-substituted hydroxyapatites, Bioceramics. 12, 191 (1999).

DOI: https://doi.org/10.1142/9789814291064_0046

[2] I. R. Gibson, S. M. Best and W. Bonfield, Chemical characterization of silicon-substituted hydroxyapatite, J. Biomed. Mater. Res 44. 422-428 (1999).

DOI: https://doi.org/10.1002/(sici)1097-4636(19990315)44:4<422::aid-jbm8>3.0.co;2-#

[3] M. Aizawa, K. Itatani, and I. Okada, Syntheses of calcium-deficient apatite fibres by a homogeneous precipitation method and their characterizations, Phosphorus Res. Bull. 20, 61 (2006).

[4] A. Yoshikawa, Y. Horiguchi, K. Oribe, and M. Aizawa, Mechanical property of apatite cement hardened by chealting-effect of inositol phosphate, Arch. BioCeram. Res. 6, 216-219 (2006).

[5] Y. Horiguchi, A. Yoshikawa, K. Oribe and M. Aizawa, Fabrication of chelate-setting hydroxyapatite cements from four kinds of commercially-available powder with various shape and crystallinity and their mechanical property, J. Ceram. Soc. Jpn. 116, 50-55 (2008).

DOI: https://doi.org/10.2109/jcersj2.116.50

[6] Y. Nakashima, M. Honda, T. Konishi, and M. Aizawa, Fabrication of chelate-setting cement using silicon-substituted hydroxyapatite and its property, Key Engineering. Mater., 370 493-494 (2011).

DOI: https://doi.org/10.4028/www.scientific.net/kem.493-494.370