Comparative Study on Bioresorbability of Chelate-Setting Cements with Various Calcium-Phosphate Phase Using Rabbit Model

Abstract:

We have developed novel calcium-phosphate cements (CPCs) based on the chelate-setting mechanism of inositol phosphate (IP6) using hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP) and α-TCP as starting materials. These cements (IP6-HAp, IP6-β-TCP and IP6-α-TCP cements) have different bioresorbability due to the chemical composition of starting materials. In the present study, biocompatibility and bioresorbability of the above three cements and commercially available cement (Biopex^®-R) was histologically evaluated in vivo using rabbit model for 4, 8, and 24 weeks, in addition to their dissolution in vitro. The dissolution of these cements increased in the order of IP6-HAp, IP6-β-TCP and IP6-α-TCP cements. The newly-formed bones were directly in contact with both the IP6-HAp and Biopex^®-R cement specimens. As for the IP6-β-TCP and IP6-α-TCP cements, newly-formed bones were formed time-dependently slightly apart from the cement specimens. Resorption rate for Biopex^®-R, IP6-HAp, IP6-β-TCP, and IP6-α-TCP cements after 24 weeks implantation were of 7.2, 5.0, 13.7, and 16.2%, respectively, compared to original cements. The present chelate-setting cements with different bioresorbability are promising candidates for application as the novel CPCs.

• Periodical: Key Engineering Materials (Volumes 529-530)
• Pages: 167-172
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.529-530.167
• Online since: November 2012
• Authors: Toshiisa Konishi, Shuhei Takahashi, Minori Mizumoto, Michiyo Honda, Koki Kida, Yukiko Horiguchi, Kazuya Oribe, Ken Ishii, Hikaru Morisue, Yoshiaki Toyama, Morio Matsumoto, Mamoru Aizawa
• Keywords: Biocompatibility, Bioresorbability, Bone Graft, Calcium Phosphate Cement, Inositol Hexaphosphate, Surface Modification

References

[1] W. E. Brown, L. C. Chow, U.S. Patent, 4, 518, 430. (1985).

[2] Y. Miyamoto, K. Ishikawa, M. Takechi, T. Toh, T. Yuasa, M. Nagayama, K. Suzuki, Histological and compositional evaluations of three types of calcium phosphate cements when implanted in subcutaneous tissue immediately after mixing, J. Biomed. Mater. Res. 48 (1999).

DOI: 10.1002/(sici)1097-4636(1999)48:1<36::aid-jbm8>3.0.co;2-i

[3] M. Aizawa, Y. Haruta, I. Okada, Development of novel cement processing using hydroxyapatite particles modified with inositol phosphate, Arch. BioCeram. Res. 3 (2003) 134–138.

[4] T. Konishi, Z. Zhuang, M. Mizumoto, M. Honda, M. Aizawa, Fabrication of chelate-setting cement from hydroxyapatite powder prepared by simultaneously grinding and surface-modifying with sodium inositol hexaphosphate and their material properties, J. Ceram. Soc. Jpn. 120 (2012).

DOI: 10.2109/jcersj2.120.159

[5] T.H. Dao, Polyvalent cation effects on myo-inositol hexakis dihydrogenphosphate enzymatic dephosphorylation in dairy wastewater, J. Environ. Qual. 32 (2003) 694–701.

DOI: 10.2134/jeq2003.6940

[6] T. Konishi, Y. Horiguchi, K. Oribe, M. Matsumoto, H. Morisue Y. Toyama, M. Aizawa, Fabrication of chelate-setting α-tricalcium phosphate cement with bioresorbability and its evaluation, Arch. BioCeram. Res. 8 (2008) 126–129.

[7] S. Takahashi, T. Konishi, K. Nishiyama, M. Mizumoto, M. Honda, Y. Horiguchi, K. Oribe, M. Aizawa, Fabrication of novel bioresorbable β-tricalcium phosphate cement on the basis of chelate-setting mechanism of inositol phosphate and its evaluation, J. Ceram. Soc. Jpn. 119 (2011).

DOI: 10.2109/jcersj2.119.35

[8] P. Ducheyne, S. Radin, L. King, The effect of calcium phosphate ceramic composition and structure on in vitro behavior. I. Dissolution, J. Biomed. Mater. Res. 27 (1993) 25–34.

DOI: 10.1002/jbm.820270105

[9] H. Oonishi, L.L. Hench, J. Wilson, F. Sugihara, E. Tsuji, S. Kushitani, H. Iwaki, Comparative bone growth behavior in granules of bioceramic materials of various sizes, J. Biomed. Mater. Res. 44 (1999) 31–43.

DOI: 10.1002/(sici)1097-4636(199901)44:1<31::aid-jbm4>3.0.co;2-9