Microfluidics Design for Single Cell Detection

Abstract:

Here we demonstrate a microfluidic-based analysis system based on single cell capture array, which can physically trap individual cell using micrometer-sized structures. A stable and in vivo-like microenvironment was built with the novel structure at the single-cell detection level. The microfluidic-based design can decouple single cells from fluid flow with the help of micropillars. The size and geometry of the cell jails are designed in order to discriminate between mother and daughter cells. It provides an experimental platform to efficiently monitor individual cell state for a long period of time. Furthermore, the parallel microfluidic array can ensure accuracy. In addition, finite element method (FEM) was employed to predict fluid transport properties for the most optimal fluid microenvironment.

• Periodical: Key Engineering Materials (Volumes 562-565)
• Pages: 589-593
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.562-565.589
• Online since: July 2013
• Authors: Shao Bo Du, Sheng Bo Sang, Wen Dong Zhang, Jie Hu, Peng Wei Li, Gang Li, Hui Feng
• Keywords: Cell Capture, Cell Detection, Finite Element Analysis Method, Micro-Fluidics

References

[1] Shengbo Sang, Hartmut Witte, A novel PDMS micro membrane biosensor based on the analysis of surface stress, Biosensors and Bioelectronics. 25 (2010), 2420-2424.

DOI: 10.1016/j.bios.2010.03.035

[2] Cao SX, Dhahbi JM, Mote PL, Spindler SR, Genomic profiling of short- and long-term caloric restriction effects in the liver of ageing mice, Proc Natl Acad Sci USA 98 (2001). 10630-10635.

DOI: 10.1073/pnas.191313598

[3] Yoon IK, Kim HK, Kim YK, et al., Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology, Exp Gerontol 39(2004). 1369-1378.

DOI: 10.1016/j.exger.2004.07.002

[4] McCarroll SA, Murphy CT, Zou S, et al., Comparing genomic expression patterns across species identifies shared transcriptional profile in ageing, Nat Genet 36(2004).197-204.

DOI: 10.1038/ng1291

[5] Michael R. Thorson, et al., A microfluidic platform for pharmaceutical salt screening, Lab on a chip. (2011).

[6] Siegal-Gaskins D, Crosson S, Tightly regulated and heritable division control in single bacterial cells. Biophys, J. 95, 2063–2072 (2008).

DOI: 10.1529/biophysj.108.128785

[7] Mettetal J T, Muzzey D, Gomez-Uribe C, van Oudenaarden A, The frequency dependence of osmoadaptation in Saccharomyces cerevisiae, Science 319 (2008). 482-484.

DOI: 10.1126/science.1151582

[8] McKnight T E. et al., Intracellular integration of synthetic nanostructures with viable cells for controlled biochemical manipulation, Nanotechnology 14, (2003). 551-556.

DOI: 10.1088/0957-4484/14/5/313

[9] Saltzman, W.M. & Olbricht, W.L., Building drug delivery into tissue engineering. Nat. Rev. Drug Discov, 1, (2002).177-186.

DOI: 10.1038/nrd744

[10] Siva A. Vanapalli, et al., Microfluidics as a functional tool for cell mechanics, Biomicrofluidics 3, (2009).

[11] Walker, G.M., H.C. Zeringue, and D.J. Beebe, Microenvironment design considerations for cellular scale studies, LAB ON A CHIP, (2004). 91-97.

DOI: 10.1039/b311214d