Nano Calcium Phosphate Powder Production through Chemical Agitation from Atlantic Deer Cowrie Shells (Cypraea cervus Linnaeus)

Abstract:

The process is a simple chemical method and aims to produce nano-structured calcium phosphate powders from natural sources, for biomedical applications. For this purpose, Atlantic Deer Cowrie (ADC) shells (Cypraea cervus Linnaeus, 1771) were collected from a local gift store in Istanbul. The empty shells were cleaned and crushed then were ball milled and sieved under 100µm. The raw powders were suspended on a hotplate stirrer for a simple chemical agitation. The temperature was kept at 80°C for 15 min. and then appropriate amount of H₃PO₄ was added by titration into the prepared solution to form calcium phosphate precursors. The solution was stirred on a hotplate for 8 hours then dried at 100°C for 24 hours. Afterwards the resulting dried sediments were collected and heat treated between 400-800°C for 4 hours, dependent on the required specific calcium phosphate phase. X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM) were carried out for identifying various hydroxyapatite (HA), tricalcium phosphate (TCP) and other calcium phosphate phases. Various particle sizes ranging from nano to micron, are obtained depending on the chemistry used and the processing technique applied during the production. A range of calcium phosphate phases can be obtained from ADC shells, by using a simple and economic conversion method. Proper cleaning methods developed and appropriate preparation techniques will enable us to use these nano calcium phosphate powders in orthopedic and dental applications.

• Periodical: Key Engineering Materials (Volume 587)
• Pages: 80-85
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.587.80
• Online since: November 2013
• Authors: Oguzhan Gunduz, Yesim Muge Sahin, Simeon Agathopoulos, Duygu Agaogullari, Hasan Gokce, E.S. Kayali, Cenk Aktas, Besim Ben-Nissan, F.N. Oktar
• Keywords: Chemical Agitation, Hydroxyapatite (HA), Natural Nanobioceramics, Sea Snail

References

[1] P. Valerio, M.M. Pereira and et al.: Biomater. Vol. 25 (2004) 2941-2948.

[2] U. Partenfelder, A. Engel and C. Russel: J. Mater. Sci.: Mater. Med. Vol. 4 (1993) 292-295.

[3] C. Ergun, Z. Evis and et al.: Ceram. Int. Vol. 37 (2011) 971-977.

[4] L.S. Ozyegin, F.N. Oktar and et al.: Mater. Lett. Vol. 58 (2004) 2605-2609.

[5] N. Demirkol, O. Meydanoglu and et al.: Acta Physica Polonica A Vol. 121 (2012) 274-276.

[6] G. Goller, F.N. Oktar and et al.: Mater. Lett. Vol., 58 (2004) 2599-2604.

[7] T.M. Coelho, E.S. Nogueira and et al.: J. Appl. Phys. Vol. 100 (2006) 094312- 094312-6.

[8] F.N. Oktar: Ceram. Int. Vol. 33 (2007) 1309-1314.

[9] N. Demirkol, M. Yetmez and et al.: Int. J. Artif. Organs Vol. 33 (2010) 468-468.

[10] M.L. Tamasan, L.S. Ozyegin and et al.: Mater. Sci. Engineer. C Vol. 33 (2013) 2569-2577.

[11] F.N. Oktar, S. Agathopoulos and et al.: Key Engineer. Mater. Vols. 529-530 (2013) 609-614.

[12] R. Samur, L.S. Ozyegin and et al.: Metalurgija, Vol. 52 (2013) 375-378.

[13] S. Agathopoulos, L.S. Ozyegin and et al.: Key Engineer. Mater. Vols. 493-494 (2012) 775-780.

[14] J.H.G. Rocha, A.F. Lemos and et al.: Bone Vol. 37 (2005) 850-857.

[15] A.F. Lemos, J.H.G. Rocha and et al.: J. Euro. Ceram. Soc. Vol. 26 (2006) 3639-3646.

[16] F.N. Oktar, U. Tuyel and et al.: Int. J. Artificial Organs Vol. 33 (2010), 467-468.

[17] L.S. Ozyegin, F. Sima and et al.: Key Eng. Mater. Vols. 493-494 (2012) 781-786.

[18] http: /en. wikipedia. org/wiki/Macrocypraea_cervus (June 22, 2013).

[19] http: /www. junglewalk. com/shop/Products/Atlantic-deer-cowrie-Poster-8074. htm#. Ub2qEI1rNjo (June 17, 2013).

[20] F.N. Oktar, I.A. Kiyici and et al., Tropical Sea Shells: Possible Exotic Sources for Ceramic, Biomaterial Synthesis International Advances in Applied Physics and Materials Science Congress, 24-28 April 2013 - Antalya, Turkey.

DOI: 10.1063/1.4849274

[21] M. Bohner, L. Galea and N. Doebelin: J. Euro. Ceram. Soc. Vol. 32 (2012) 2663-2671.

[22] D. Kel, U. Karacayli and et al.: Int. J. Artif. Organs Vol. 34 (2011) 700.

[23] D. Kel, H. Gökçe and et al.: Key Eng. Mater. Vols. 493-494 (2012) 287-292.

[24] S.Y.J. Loo, Y.E. Siew and et al.: J. Mater Sci. Mater. Med. Vol. 19 (2008) 1389-1397.

[25] http: /en. wikipedia. org/wiki/Portlandite (June 30, 2013).

[26] Biomaterials: A Tantalus Experience (Biological and Medical Physics, Biomedical Engineering) Eds. Jozef A. Helsen and Yannis Missirlis, Springer – Heidelberg – Dordrecht – London – New York; 2010 edition (November 5, 2010).

DOI: 10.1007/978-3-642-12532-4

[27] W.E. Brown and E.F. Epstein: J. Res. Nat. Bureau Standards - A. Phys. Chem., Vol. 69A, (1965) 547-551.

[28] M. Bohner: Euro. Cells Mater. Vol. 20 (2010) 1-12.

[29] S.V. Dorozhkin: BIO Vol. 1 (2011) 1-51.