The Potential of Garlic Extract as Bio-Inhibitor in Urea Fertilizer

Abstract:

Urea is extensively used as fertilizer in the agricultural industry based on its suitability for all types of crops. The hydrolysis of urea fertilizer produces ammonia (NH₃) and carbon dioxide (CO₂). However, up to 40% of NH₃ release affects the efficiency of urea fertilizer. By introducing inhibitors into the urea enzymatic reaction, the NH₃ emission problem can be solved. Unfortunately, current inhibitors are usually chemical based and non-biodegradable. Several complaints and accidents have been reported when handling chemical based inhibitors especially for surface application. Research on garlic or Allium savatium has been conducted to ensure its inhibitory effects as potentially safe and biodegradable inhibitor. From previous research, thiosulfinates (TS) contained in garlic extract proved to inhibit platelets aggregation in medical applications. In this study, the inhibitory effect is determined by analyzing NH₃ concentration in urease-garlic mixture and standard urea assay mixtures using UV-VIS spectrophotometer device. Previous research showed the highest absorbance of free NH₃ was detected at 370nm. At 30 minutes of 15ml of urease-garlic mixture confirms the fully inhibition of garlic extract towards reaction.

• Periodical: Key Engineering Materials (Volumes 594-595)
• Pages: 296-300
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.594-595.296
• Online since: December 2013
• Authors: Sity Juaeiriah Samsudin, Nurlidia Binti Mansor, Suriati Sufian, Zakaria B. Man
• Keywords: Ammonia, Garlic Extract, Inhibition, Thiosulfinate, UV-Vis Spectrophotometer

References

[1] Explosion Hazard from Ammonium Nitrate. (1997). In Environmental Protection Agency. Accidents. United States.

[2] E. Funderburg (2009). Nitrogen Loss from Urea. Retrieved on May 2009 from http: /www. noble. org/ag/soils/nitrogenlosses.

[3] S. Jongeneel (2012). Nitrogen applications for the 2012 corn crop. Retrieved from May 21, 2012 from www. agprofressional. com/news/Nitrogen-applications-for-the-2012-corn-crop-152075175. html.

[4] F. Fernandez (2011). Late season nitrogen application on corn. Retrieved from http: /bulletin. ipm. illinois. edu.

[5] Varsa et al. (1998). An Evaluation of Urease Inhibitor Technology as a Nitrogen Management Tools in No-Till Corn and Wheat Production. Retrieved on January 26-28, 1998 from frec. ifra. com/1998/report1.

[6] Weyerhaeuser (2012). Fertilizer Technology Broucher. Retrieved from www. arboritefertilizer. com.

[7] Zaborska, W. et al. (2009). Modification of jack bean urease thiols by thiolsulfinates contained in garlic extract: DTNB titration studies. Retrieved on November 12, 2007 from www. elsevier. com/locate/foodchem.

DOI: 10.1016/j.foodchem.2008.05.029

[8] P. M. Tejo et al. (2011). The effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium into Triticum aestivum L. Plant Growth Regulation. 63: pp.73-79.

DOI: 10.1007/s10725-010-9513-6

[9] N-(n-butyl) thiophosphoric triamide (NBPT). (2010). In Australian Government, Department of Health and Ageing, Existing Chemical Secondary Notification Assessment. Australia.

[10] Juszkiewicz et al. (2004). A study of the inhibition of jack bean urease by garlic extract. (Research Report, pp.553-558). Retrieved from http: /www. sciencedirect. com/science/article/pii/S0308814603003868.

DOI: 10.1016/j.foodchem.2003.07.033

[11] Palmer, Ross & Nutt (2001). Measuring Ammonia with Online Analyzers. Retrieved from http: /www. wqpmag. com/measuring-ammonia-online-analyzers.

[12] T. Merian et al., (2009).

[13] Z. Olech & W. Zaborska. (2012). A Spectrophotometric Assay for Total Garlic Thiosulfinates Content. Kinetic Aspects of Reaction with Chromogenic Thiols. (Research Report, vol. 62, pp.23-29). Retrieved from http: /journal. pan. olsztyn. pl.

DOI: 10.2478/v10222-011-0042-4

[14] H.P. Dong et al. (2010).