Poly (L-Lactic Acid) and Hydroxyapatite Scaffold for Bone Regeneration: In Vivo Study

Abstract:

In bone tissue engineering, synthetic scaffolds are commonly used and this should present the following requirements; (i) recapitulate the native three-dimensional (3D) hierarchical fibrous structure, (ii) possess biomimetic surface properties and (iii) demonstrate mechanical integrity. However, some methods of producing scaffolds do not achieve these requirements. The present study aims the application of a composite of poly (L-lactic acid) (PLLA) and Hydroxyapatite (HA) produced by rotary jet spinning, which can be used to obtain scaffolds that meet the above requirements with affordable costs (regarding materials and production). The morphology and thermal properties of the scaffolds were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). For the in vivo tests, 20 Wistar rats, distributed into two groups, in which critical defects were performed in cranial calotte were used. Then scaffolds of PLLA/HA were implanted and compared with the control group that didn’t receive the implant. The results have shown that in the cases where only the defects in cranial caps were performed, bone healing did not occur. In cases where the scaffolds of PLLA/HA were used, rich neovascularization was noted, accompanied by foreign body type reaction and presence of reactive bone around the implants. The evaluation of PLLA/HA scaffolds used in the rat calvarial defect model, according to the criteria surveyed was favorable, showed the implants insurance and that they are suitable materials to be used as substitutes of calvarial bone tissue in these animals.

• Periodical: Key Engineering Materials (Volume 631)
• Pages: 435-440
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.631.435
• Online since: November 2014
• Authors: Geraldine N.P. Rodriguez*, Leonardo R. Rodrigues, Rafael C.F. Basso, Paulo Kharmandayan, Cecília A.C. Zavaglia, Marcos A. D'Ávila
• Keywords: Implanted Scaffold, Rat Calvarial Defect, Rotary Jet Spinning, Tissue Regeneration

References

[1] P. P. Spicer, J. D. Kretlow, S. Young, J. A. Jansen, F. K. Kasper1, A. G. Mikos, Evaluation of bone regeneration using the rat critical size calvarial defect, Nature Protocols VOL. 7 NO. 10 (2012) 1918-(1929).

DOI: 10.1038/nprot.2012.113

[2] Y. Gu, W. Huang, M. N. Rahaman, D.E. Day, Bone regeneration in rat calvarial defects implanted with fibrous scaffolds composed of a mixture of silicate and borate bioactive glasses, Acta Biomaterialia 9 (2013) 9126–9136.

DOI: 10.1016/j.actbio.2013.06.039

[3] A. R. Shrivats, M. C. McDermott, J. O. Hollinger, Bone tissue engineering: state of the union, Drug Discovery Today: Volume 19, Number 6, June (2014).

DOI: 10.1016/j.drudis.2014.04.010

[4] M.D. Schofer, L. Tunnermann, H. Kaiser , P.P. Roessler, C. Theisen, J.T. Heverhagen, J. Hering, M. Voelker, S. Agarwal, T. Efe, S. Fuchs-Winkelmann, J.R.J. Paletta, Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo, J. Mater Sci.: Mater Med (2012).

DOI: 10.1007/s10856-012-4697-0

[5] C. Romagnoli, M. L. Brandi, Adipose mesenchymal stem cells in the field of bone tissue engineering, World J Stem Cells (2014) April 26; 6(2): 144-152.

DOI: 10.4252/wjsc.v6.i2.144

[6] L. D. Swain1, D. A. Cornet, M. E. Manwaring1, B. Collins, V. K. Singh1, D. Beniker, D. L. Carnes, Negative pressure therapy stimulates healing of critical-size calvarial defects in rabbits, BoneKEy Reports 2, Article number: 299 (2013).

DOI: 10.1038/bonekey.2013.33

[7] M. R. Badrossamay, K. Balachandran, A. K. Capulli , H. M. Golecki, A. Agarwal, J. A. Goss, H. Kim, K. Shin, K. K. Parker, Engineering hybrid polymer-protein super-aligned nanofibers via rotary jet spinning, Biomaterials 35 (2014) 3188e3197.

DOI: 10.1016/j.biomaterials.2013.12.072

[8] S. Wua, X. Liu, K. W.K. Yeung, C. Liu, X. Yang, Biomimetic porous scaffolds for bone tissue engineering, Materials Science and Engineering R 80 (2014) 1–36.

DOI: 10.1016/j.mser.2014.04.001

[9] M.R. Badrossamay, H.A. McIlwee, J.A. Goss, K.K. Parker, Nanofiber assembly by rotary jet-spinning. Nano Lett (2010)10: 2257e61.

DOI: 10.1021/nl101355x

[10] B. Chuenjitkuntaworn,W. Inrung, D. Damrongsri, K. Mekaapiruk, P. Supaphol, P. Pavasant, Polycaprolactone/Hydroxyapatite composite scaffolds: Preparation, characterization, and in vitro and in vivo biological responses of human primary bone cells, Journal of Biomedical Materials Research Part A (2010).

DOI: 10.1002/jbm.a.32657

[11] L.R. Rodrigues, M. Motisuke, C.A.C. Zavaglia, Synthesis of nanostructured hydroxyapatite: A comparative study between sol-gel and aqueous solution precipitation, Key Engineering Materials, 396-398 (2009) 623-626.

DOI: 10.4028/www.scientific.net/kem.396-398.623

[12] A.C. Motta, E. A.R. Duek, Sintese, caracterizacão e degradacão In Vitro, do Poli(L-ácido láctico), Polimeros: Ciencia e Tecnologia. Vol 16: numero 001. pp.26-32. (2006).

DOI: 10.1590/s0104-14282006000100008

[13] R. C. F. Basso, Uso de scaffolds de poli L lactide e poli L lactide com hidroxiapatita em calota craniana: modelo experimental em ratos Wistar, (2014) Dissertação (Mestrado em Ciências Médicas), Universidade Estadual de Campinas, Campinas.

DOI: 10.47749/t/unicamp.2014.927633