Overexpression and Characterization of the Gene Encoding 1,3-Propanediol Oxidoreductase of Citrobacter freundii

Xiang Hui Qi; Jing Fei Zhu; Yan Luo; Jing Lin; Xu Wang; Zhao Ju; Fang Chen; Xiao Guan Zhu; Hua You Chen; Wen Jing Sun; Liang Wang

doi:10.4028/www.scientific.net/KEM.636.121

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 636Overexpression and Characterization of the Gene...

Overexpression and Characterization of the Gene Encoding 1,3-Propanediol Oxidoreductase of Citrobacter freundii

Abstract:

Glycerol can be biologically converted to 1,3-propanediol (1,3-PD), a key raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers. 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-PD synthesis biologically pathway. In present study, the dhaT gene encoding PDOR was cloned from Citrobacter freundii and overexpressed in E. coli BL21. The recombinant enzyme was purified by nickel-chelate chromatography combined with gel filtration to study the enzymatic characterization. Specific activity of recombinant PDOR was 55.2 U/mg. K_m and V_max values were 8.9 mM, 40.2 U/mg respectively. Holoenzyme of PDOR maybe a decamer of which a monomer has a molecular mass of 43 kDa. This research may form a basis for the future work on biological synthesis of 1, 3-PD.

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Periodical:

Key Engineering Materials (Volume 636)

Pages:

121-124

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.636.121

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Online since:

December 2014

Authors:

Xiang Hui Qi*, Jing Fei Zhu, Yan Luo, Jing Lin, Xu Wang, Zhao Ju, Fang Chen, Xiao Guan Zhu, Hua You Chen, Wen Jing Sun, Liang Wang

Keywords:

Biosynthesis, Characterization, Enzymes, Kinetic, Overexpression

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