Facile Method of Enzyme Immobilization on Silica Quasi-Nanospheres (SiO2) Synthesized Using PODFA for Biosensing Applications

Nurulaina Fasya Saiful Anuar; Halina Misran; Musdalilah Ahmad Salim; S.Z. Othman

doi:10.4028/www.scientific.net/KEM.694.67

Paper Titles

Synthesis and Characterizations of MOF-199 Using PODFA as Porogen for CO₂ Adsorption Applications
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Facile Method of Enzyme Immobilization on Silica Quasi-Nanospheres (SiO₂) Synthesized Using PODFA for Biosensing Applications
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 694Facile Method of Enzyme Immobilization on Silica...

Facile Method of Enzyme Immobilization on Silica Quasi-Nanospheres (SiO₂) Synthesized Using PODFA for Biosensing Applications

Abstract:

In this study, silica spheres (SiO₂) nanomaterials were introduced as the support materials for glucose sensors. Glucose oxidase (GOx) was immobilized onto SiO₂ spheres (SiO₂/GOx) using physical adsorption (electrostatic interaction) method. The SiO₂ spheres were synthesized in-house using a nonsurfactant template of palm oil derived fatty alcohols (PODFA). Based on FT-IR analyses, SiO₂/GOx exhibited N-H absorbance at ca. 2990 cm^-1 and O-H absorbance at ca. 3330 cm^-1. One new absorbance peak was observed at ca. 2320 cm¹ attributed to the bending vibration (ν_bend)of silane molecules (Si-H) obtained by the interaction of GOx and the surface silanols (Si-OH). UV-Vis analysis results exihibited the presence of a new broad peak for GOx/SiO₂ sample at ca. 250 nm to 280 nm suggested to be assigned to H₂O_2,C=C and benzene ring from GOx. The optimum pH and optimum temperature for SiO₂/GOx for GOx activity were ca. 7.0 and ca. 50°C respectively. The increase of glucose concentration from 2mM to 5mM resulted to the increment of absorbance value for both GOx and SiO₂/GOxsuggesting succesful immobilization of GOx on SiO₂spheres. These materials were suitable for glucose detection at very small glucose concentrations particularly in salivary glucose detection.

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Key Engineering Materials (Volume 694)

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67-71

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https://doi.org/10.4028/www.scientific.net/KEM.694.67

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May 2016

Authors:

Nurulaina Fasya Saiful Anuar*, Halina Misran, Musdalilah Ahmad Salim, S.Z. Othman

Keywords:

Glucose Biosensor, Immobilization, Silica Spheres

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