Naturally Derived Matrix for Controlled Selenium Nanoparticles Delivery

Abstract:

The aim of this study is to develop a lyophilized matrix (microspheres) as a controlled delivery system for nanoselenium particles, using different formulation based on alginate or agar. Elemental selenium is considered as the least toxic of all selenium forms and in the same time supplementation with its nanosize particles has the same or better bioavailability compared to its salts. In our study, nanosized elemental selenium was obtained by fermentation technology using probiotic lactic acid bacteria (Lactobacillus casei). The microspheres have been characterized from structural point of view by using different techniques: FTIR spectroscopy, X-ray Diffraction and SEM. Each individual natural polymer has its own characteristic advantages and disadvantages; it is commonly accepted that naturally derived matrix often show an excellent balance between the mechanical properties, swelling and dissolution capacity. The optimized formulation was proposed upon in vitro dissolution study using Diferential Pulsed Voltammetry in order to measure the concentration of selenium released in simulated gastric fluid (pH=1.2) and simulated intestinal fluid (pH=8.1). The cumulative release of selenium from different formulations showed large differences with respect to matrix composition. We demonstrated that both alginate and agarose-based formulations are suitable to be used in basic environment such as small or large intestine. The results might be of high importance as absorption of selenium occurs mainly in the duodenum, caecum and colon (more than 85%).

• Periodical: Key Engineering Materials (Volume 695)
• Pages: 284-288
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.695.284
• Online since: May 2016
• Authors: Simona Cavalu*, Vasile Laslo, Florin Banica, Simona Ioana Vicas
• Keywords: Biopolymers, Controlled Delivery, Nanoparticles

References

[1] H. -F. Ko, C. Sfeir, P. N. Kumta, Novel synthesis strategies for natural polymer and composite biomaterials as potential scaffolds for tissue engineering, Phil. Trans. R. Soc. A (2010) 368, 1981–(1997).

DOI: 10.1098/rsta.2010.0009

[2] H. -R. Lin,Y. -J. Yeh, Porous alginate/hydroxyapatite composite scaffolds for bone tissue engineering: preparation, characterization, and in vitro studies. J. Biomed. Mater. Res. B: Appl. Biomater (2004)71, 52–65.

DOI: 10.1002/jbm.b.30065

[3] A. Perets, Y. Baruch, F. Weisbuch, G. Shoshany, G. Neufeld, S. Cohen, Enhancing the vascularization of three-dimensional porous alginate scaffolds by incorporating controlled release basic fibroblast growth factor microspheres. J. Biomed. Mater. Res. A (2003).

DOI: 10.1002/jbm.a.10542

[4] J. H. Guo, G.W. Skinner, W.W. Harcum, P.E. Barnum, Pharmaceutical applications of naturally occurring water-soluble polymers. Pharm. Sci. Technol. Today (1998)1, 254–261.

DOI: 10.1016/s1461-5347(98)00072-8

[5] J.S. Boateng, D. Areago, Composite Sodium Alginate and Chitosan Based Wafers for Buccal Delivery of Macromolecules, Austin J Anal Pharm Chem (2014)1(5): 1022.

[6] J. -W. Rhim, S-B. Lee, S. -I Hong, Preparation and Characterization of Agar/Clay Nanocomposite Films: The Effect of Clay Type, Journal of Food Science (2011)76, 3, 40-48.

DOI: 10.1111/j.1750-3841.2011.02049.x

[7] A.C. Aufderheide, K.A. Athanasiou, Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus. Tissue Eng (2005)11, 1095–1104.

DOI: 10.1089/ten.2005.11.1095

[8] X.L. Xu, J. Lou, T. Tang, K.W. Ng, J. Zhang, C. Yu, K. Dai, Evaluation of different scaffolds for BMP-2 genetic orthopedic tissue engineering. J. Biomed. Mater. Res. B Appl. Biomater (2005)75, 289–303.

DOI: 10.1002/jbm.b.30299

[9] O. Khanna, J.C. Larson, M.L. Moya, E.C. Opara, E.M. Brey, Generation of Alginate Microspheres for Biomedical Applications. J. Vis. Exp. (2012) 66, e3388, doi: 10. 3791/3388.

DOI: 10.3791/3388-v

[10] M. Roman, P. Jitaru,C. Barbante, Selenium biochemistry and its role for human health, Metallomics (2014) 6(1): 25–54.

DOI: 10.1039/c3mt00185g

[11] D.L. Hatfield, P.A. Tsuji, B.A. Carlson, V.N. Gladyshev, Selenium and selenocysteine: roles in cancer, health, and development, Trends Biochem Sci (2014) 39(3): 112–120.

DOI: 10.1016/j.tibs.2013.12.007

[12] P. Eszenyi, A. Sztrik, B. Babka, J. Prokisch, Elemental, Nano-Sized (100-500 nm) Selenium Production by Probiotic Lactic Acid Bacteria, International Journal of Bioscience, Biochemistry and Bioinformatics (2011) 1(2)148-152.

DOI: 10.7763/ijbbb.2011.v1.27

[13] S. -Ad Riyajan, J. Nuim, Interaction of Green Polymer Blend of Modified Sodium Alginate and Carboxylmethyl Cellulose Encapsulation of Turmeric Extract, International Journal of Polymer Science (2013) http: /dx. doi. org/10. 1155/2013/36425.

DOI: 10.1155/2013/364253

[14] C. Reilly, Selenium in food and health, Springer Science &Busines Media, New York, (2013).