Paper Titles

Tissue Reaction to Subcutaneous Implantation of SuperEBA (Reinforced Zinc Oxide Cement) Used as Root-End Filling Material: A Histological Study in Rats
p.247

Structural and Ultrastructural Study, at Ovariectomised Female Rats, and their Reactivity to the Administration of Injectable Oestrogens
p.252

Histologic Evaluation of Tertiary Dentine after Indirect Pulp Capping Procedures
p.260

The Influence of Filler in Composite Cellulose Acetate Membranes for Proteins Recovery
p.267

Chemical Crosslinking of Silk Fibroin, Chitosan and Gelatin Blend Nanofiber Mats
p.273

The Potential of NDPs-Loaded Fish Gelatin Fibers as Reinforcing Agent for Fish Gelatin Hydrogels
p.278

Naturally Derived Matrix for Controlled Selenium Nanoparticles Delivery
p.284

Bioactive Compounds Diffusion from Grapes to Wines under High Frequency Electromagnetic Field Treatments
p.289

Impact of Electromagnetic Radiation on the Human Organism
p.295

HomeKey Engineering MaterialsKey Engineering Materials Vol. 695Chemical Crosslinking of Silk Fibroin, Chitosan...

Chemical Crosslinking of Silk Fibroin, Chitosan and Gelatin Blend Nanofiber Mats

Article Preview

Abstract:

Silk fibroin (SF), chitosan (C), and gelatin (G) blend composition in formic acid have been manufactured by using electrospinning technique in our previous work. The SF: G: C at weight ratios of 10: 20: 1 and 20: 10: 1 (%wt: %wt: ml) were used for crosslinking testing. Glutaraldehyde (GA), and 1-ethyl-3-(3-dimethylaminopropyl) cabodiimide (EDC) / N-hydroxy succcinimide (NHS) (EDC/NHS) were chose as crosslinking agents. All samples were treated in such agents by fumigation for 72 hours followed by dipping for 10 minutes. Then those samples were washed in distill water for 3 times (1 time per 10 minutes) and dried in desiccator at room temperature. GA caused immediately shrink in both nanofiber mats and became clearly visible yellowish. However, a little shrinkage occurred after dipping in EDC/NHS. It possibly concluded that the EDC / NHS appropriated for SF: G: C blend nanofiber mats.

You might also be interested in these eBooks

Biomaterials and Regenerative Biomedicine

Info:

Periodical:

Key Engineering Materials (Volume 695)

Pages:

273-277

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.695.273

Citation:

Cite this paper

Online since:

May 2016

Authors:

Pornpen Siridamrong, Penchom Phrotjanatharee, Niyom Thamronganaskul

Keywords:

Chemical Crosslinking, Chitosan, Electrospinning, Gelatin, Silk Fibroin

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2016 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] A.L. Andrady, Applications of Nanofiber Mats, Science and Technology of Polymer Nanofibers, John Wiley & Sons, Inc. 2007, pp.225-247.

[2] K.H. Kim, L. Jeong, H.N. Park, S.Y. Shin, W.H. Park, S.C. Lee, T.I. Kim, Y.J. Park, Y.J. Seol, Y.M. Lee, Y. Ku, I.C. Rhyu, S.B. Han, C.P. Chung, Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration, Journal of biotechnology, 120 (2005).

DOI: 10.1016/j.jbiotec.2005.06.033

[3] X. Liu, P.X. Ma, Phase separation, pore structure, and properties of nanofibrous gelatin scaffolds, Biomaterials, 30 (2009) 4094-4103.

DOI: 10.1016/j.biomaterials.2009.04.024

[4] R. Jayakumar, M. Prabaharan, P.T. Sudheesh Kumar, S.V. Nair, H. Tamura, Biomaterials based on chitin and chitosan in wound dressing applications, Biotechnology advances, 29 (2011) 322-337.

DOI: 10.1016/j.biotechadv.2011.01.005

[5] K. Yoon, H.N. Lee, C.S. Ki, D. Fang, B.S. Hsiao, B. Chu, I.C. Um, Effects of degumming conditions on electro-spinning rate of regenerated silk, International journal of biological macromolecules, 61 (2013) 50-57.

DOI: 10.1016/j.ijbiomac.2013.06.039

[6] J.S. Ko, K. Yoon, C.S. Ki, H.J. Kim, D.G. Bae, K.H. Lee, Y.H. Park, I.C. Um, Effect of degumming condition on the solution properties and electrospinnablity of regenerated silk solution, International journal of biological macromolecules, 55 (2013).

DOI: 10.1016/j.ijbiomac.2012.12.041

[7] Z. -M. Huang, Y.Z. Zhang, S. Ramakrishna, C.T. Lim, Electrospinning and mechanical characterization of gelatin nanofibers, Polymer, 45 (2004) 5361-5368.

DOI: 10.1016/j.polymer.2004.04.005

[8] C.S. Ki, D.H. Baek, K.D. Gang, K.H. Lee, I.C. Um, Y.H. Park, Characterization of gelatin nanofiber prepared from gelatin–formic acid solution, Polymer, 46 (2005) 5094-5102.

DOI: 10.1016/j.polymer.2005.04.040

[9] M. -H. Ho, C. -C. Hsieh, S. -W. Hsiao, D. Van Hong Thien, Fabrication of asymmetric chitosan GTR membranes for the treatment of periodontal disease, Carbohydrate Polymers, 79 (2010) 955-963.

DOI: 10.1016/j.carbpol.2009.10.031

[10] F. Croisier, C. Jérôme, Chitosan-based biomaterials for tissue engineering, European Polymer Journal, 49 (2013) 780-792.

DOI: 10.1016/j.eurpolymj.2012.12.009

[11] H. Nagahama, H. Maeda, T. Kashiki, R. Jayakumar, T. Furuike, H. Tamura, Preparation and characterization of novel chitosan/gelatin membranes using chitosan hydrogel, Carbohydrate Polymers, 76 (2009) 255-260.

DOI: 10.1016/j.carbpol.2008.10.015

[12] C. Huang, R. Chen, Q. Ke, Y. Morsi, K. Zhang, X. Mo, Electrospun collagen–chitosan–TPU nanofibrous scaffolds for tissue engineered tubular grafts, Colloids and Surfaces B: Biointerfaces, 82 (2011) 307-315.

DOI: 10.1016/j.colsurfb.2010.09.002

[13] C. Meechaisue, P. Wutticharoenmongkol, R. Waraput, T. Huangjing, N. Ketbumrung, P. Pavasant, P. Supaphol, Preparation of electrospun silk fibroin fiber mats as bone scaffolds: a preliminary study, Biomedical materials (Bristol, England), 2 (2007).

DOI: 10.1088/1748-6041/2/3/003

[14] S. Wang, G. Zhao, Quantitative characterization of the electrospun gelatin–chitosan nanofibers by coupling scanning electron microscopy and atomic force microscopy, Materials Letters, 79 (2012) 14-17.

DOI: 10.1016/j.matlet.2012.03.044

[15] M.Z. Elsabee, H.F. Naguib, R.E. Morsi, Chitosan based nanofibers, review, Materials Science and Engineering: C, 32 (2012) 1711-1726.

DOI: 10.1016/j.msec.2012.05.009

[16] K. Ohkawa, D. Cha, H. Kim, A. Nishida, H. Yamamoto, Electrospinning of Chitosan, Macromolecular Rapid Communications, 25 (2004) 1600-1605.

DOI: 10.1002/marc.200400253

[17] Y. Zhou, H. Yang, X. Liu, J. Mao, S. Gu, W. Xu, Electrospinning of carboxyethyl chitosan/poly(vinyl alcohol)/silk fibroin nanoparticles for wound dressings, International journal of biological macromolecules, 53 (2013) 88-92.

DOI: 10.1016/j.ijbiomac.2012.11.013

[18] A. Bigi, G. Cojazzi, S. Panzavolta, K. Rubini, N. Roveri, Mechanical and thermal properties of gelatin films at different degrees of glutaraldehyde crosslinking, Biomaterials, 22 (2001) 763-768.

DOI: 10.1016/s0142-9612(00)00236-2

[19] Q. Yu, Y. Song, X. Shi, C. Xu, Y. Bin, Preparation and properties of chitosan derivative/poly(vinyl alcohol) blend film crosslinked with glutaraldehyde, Carbohydrate Polymers, 84 (2011) 465-470.

DOI: 10.1016/j.carbpol.2010.12.006

[20] V. Sencadas, D.M. Correia, C. Ribeiro, S. Moreira, G. Botelho, J.L. Gómez Ribelles, S. Lanceros-Mendez, Physical-chemical properties of cross-linked chitosan electrospun fiber mats, Polymer Testing, 31 (2012) 1062-1069.

DOI: 10.1016/j.polymertesting.2012.07.010

[21] I. Kołodziejska, B. Piotrowska, The water vapour permeability, mechanical properties and solubility of fish gelatin–chitosan films modified with transglutaminase or 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and plasticized with glycerol, Food Chemistry, 103 (2007).

DOI: 10.1016/j.foodchem.2006.07.049

[22] P. Siridamrong, S. Swasdison, N. Thamrongananskul, Preparation and Characterization of Polymer Blends from Nang noi srisaket 1 Silk Fibroin, Gelatin and Chitosan Nanofiber Mats Using Formic Acid Solution, Key Engineering Materials, 659 (2015).

DOI: 10.4028/www.scientific.net/kem.659.28

[23] Z. -x. Cai, X. -m. Mo, K. -h. Zhang, L. -p. Fan, A. -l. Yin, C. -l. He, H. -s. Wang, Fabrication of Chitosan/Silk Fibroin Composite Nanofibers for Wound-dressing Applications, International Journal of Molecular Sciences, 11 (2010) 3529.

DOI: 10.3390/ijms11093529