Thin Degradable Coatings for Optimization of Osteointegration Associated with Simultaneous Infection Prophylaxis

Anke Bernstein; Norbert Suedkamp; Hermann Otto Mayr; Rainer Gadow; Irina Arhire; Andreas Killinger; Peter Krieg

doi:10.4028/www.scientific.net/KEM.696.171

Paper Titles

Fabrication of Bioactive Stainless Steel by the Function of Apatite Nuclei
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Increased Pre-Osteoblast Bioactivity on Ca-Modified Titanium
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Mesoporous Silica Coating on Rf-Sputtered Apatite/Titanium Substrate
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Primary Stability of Uncemented Total Hip Stems with Different Ceramic and Titanium Surface Coatings
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Thin Degradable Coatings for Optimization of Osteointegration Associated with Simultaneous Infection Prophylaxis
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The Balance between Bone Formation and Material Resorption in Unidirectional Porous β-Tricalcium Phosphate Implanted in a Rabbit Tibia
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Effects of Na Alginate in the Porosity of Scaffold Biphasic Calcium Phosphate/Alginate Composites
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Highly Porous β-TCP Block with Triple Pore Structure in Rat Subcutaneous Tissue and Sheep Iliac Critical Bone Defect
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Quantitative Estimation of Connectivity among Pores in the Hydroxyapatite Scaffold Prepared by Slip Casting with Porogen
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 696Thin Degradable Coatings for Optimization of...

Thin Degradable Coatings for Optimization of Osteointegration Associated with Simultaneous Infection Prophylaxis

Abstract:

The colonization of biomaterials with bacteria represents the main cause of implant-associated infections. Both an antibiotic prophylaxis and a faster osteointegration can be obtained by incorporation of bactericidal active metals in degradable CaP coatings. At present there is no reliable method on the basis of thermal spraying to get thin homogeneous layers containing silver, copper and bismuth in bacteriostatic / bactericidal concentrations. The aim of the study was the development and optimization of high-velocity suspension flame spraying (HVSFS) process for producing thin resorbable bioactive ceramics coatings on the basis of degradable calcium phosphates. In these layers the bacteriostatic / bactericidal effective metal copper should be integrated. Cells were grown on the materials for 3, 7, 14, and 21 days. Live/dead assay was used to measure cell viability. The in vitro cytotoxicity was determined by the microculture tetrazolium (WST) assay. Cell morphology, cell attachment, and cell spreading were investigated using laser scanning microscopy and raster scanning electron microscopy. All substrates supported sufficient cellular growth for 21 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells was verified

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Periodical:

Key Engineering Materials (Volume 696)

Pages:

171-176

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.696.171

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Online since:

May 2016

Authors:

Anke Bernstein*, Norbert Suedkamp, Hermann Otto Mayr, Rainer Gadow, Irina Arhire, Andreas Killinger, Peter Krieg

Keywords:

Biocompatibility, Bone, Coating, High Velocity Suspension Flame Spraying, Infection

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References

[1] I. Polyzois, D. Nikolopoulos, I. Michos, E. Patsouris, S. Theocharis. Local and systemic toxicity of nanoscale debris particles in total hip arthroplasty, J. Appl. Toxicol. 32(2012) 255-69.

DOI: 10.1002/jat.2729

Google Scholar

[2] S. Kurtz, K. Ong, E. Lau, et al. Projections of primary and revision hip and knee arthroplasty in the United States from 2005 to 2030. J Bone Joint Surg Am 89 (2007) 780–785.

DOI: 10.2106/jbjs.f.00222

Google Scholar

[3] Information on http: /www. shpr. se/en/Publications/DocumentsReports. aspx, Annual Report (2013).

Google Scholar

[4] A. Bernstein, D. Nöbel, H.O. Mayr, G. Berger, G. Gildenhaar, J. Brandt. Histological and Histomorphometric Investigations on Bone Integration of Rapidly Resorbable Calcium Phosphate Ceramics, Biomed. Mater. Res. B Appl Biomater. 84(2008).

DOI: 10.1002/jbm.b.30891

Google Scholar

[5] Montanaro L, Speziale P, Campoccia D, Ravaioli S, Cangini I, Pietrocola G, Giannini S, Arciola CR. Scenery of Staphylococcus implant infections in orthopedics. Future Microbiol. 2011 Nov; 6(11): 1329-49.

DOI: 10.2217/fmb.11.117

Google Scholar

[6] J. Baker, S. Sitthisak, M. Sengupta, M. Johnson, R.K. Jayaswal, J.A. Morrissey, Copper stress induces a global stress response in Staphylococcus aureus and represses sae and agr expression and biofilm formation, Appl. Environ. Microbiol. 76 (2010).

DOI: 10.1128/aem.02268-09

Google Scholar

[7] G. Bolelli, V. Cannillo, R. Gadow, A. Killinger, L. Lusvarghi, J. Rauch, Microstructural and in vitro characterisation of high-velocity suspension flame sprayed (HVSFS) bioactive glass coatings, J. Eur. Ceram. Soc. 29 (2009) 2249–57.

DOI: 10.1016/j.jeurceramsoc.2009.01.032

Google Scholar

[8] G. Bolelli, V. Cannillo, R. Gadow, A. Killinger, L. Lusvarghi, J. Rauch, M. Romagnoli, Effect of the suspension composition on the microstructural properties of high velocity suspension flame sprayed (HVSFS) Al2O3 coatings, Surf. Coat. Technol. 204 (2010).

DOI: 10.1016/j.surfcoat.2009.10.045

Google Scholar

[9] W.H. Lee, C.Y. Loo, R. Rohanizadeh, A review of chemical surface modification of bioceramics: effects on protein adsorption and cellular response. Colloids. Surf. B. Biointerfaces, 122 (2014) 823-34.

DOI: 10.1016/j.colsurfb.2014.07.029

Google Scholar

[10] P. Ducheyne,Q. Qiu. Bioactive ceramics, The effect of surface reactivity on bone formation and bone cell function. Biomaterials 2(1999) 2287–2303.

DOI: 10.1016/s0142-9612(99)00181-7

Google Scholar

[11] C. Ohtsuki, M Kamitakahara, T. Miyazaki, Bioactive ceramic-based materials with designed reactivity for bone tissue regeneration, J. R. Soc. Interface, 2009 6 (2009) 349-60.

DOI: 10.1098/rsif.2008.0419.focus

Google Scholar