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Effect of Isoelectric Point on Enzyme Immobilization Property of Magnetic Apatite Microcapsules Encapsulating Maghemite
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Effect of Isoelectric Point on Enzyme Immobilization Property of Magnetic Apatite Microcapsules Encapsulating Maghemite

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Abstract:

It is well-known that apatite has high affinity to many kinds of biomolecules. In this study, the authors aimed to develop the apatite microcapsules with both enzyme immobilization property by the bioaffinity of apatite and magnetism of core materials. The authors encapsulated maghemite particles, possesing ferrimagnetism, with apatite by attaching Apatite Nuclei on the surfaces of maghemite particles and subsequently immersing them in SBF. To evaluate performance of the microcapsules as enzyme immobilization carriers, the authors investigated enzyme immobilization property of the microcapsules fabricated by biomimetic method using Apatite Nuclei and SBF from the viewpoint of the difference of isoelectric point of the three-types of enzymes.

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Periodical:

Key Engineering Materials (Volume 758)

Pages:

178-183

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.758.178

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Online since:

November 2017

Authors:

Masaya Yamamoto, Takeshi Yabutsuka*, Shigeomi Takai, Takeshi Yao

Keywords:

Apatite Microcapsules, Apatite Nuclei, Enzyme Immobilization, Maghemite, Magnetic Property

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References

[1] K. Kandori, S. Mizumoto, S. Toshima, M. Fukusumi, Y. Morisada, Effects of Heat Treatment of Calcium Hydroxyapatite Particles on the Protein Adsorption Behavior, J. Phys. Chem. B, 113 (2009) 11016-11022.

DOI: 10.1021/jp904481z

Google Scholar

[2] Y. Ma, J. Zhanga, S. Guo, J. Shi, W. Du, Z. Wang, L. Ye, W. Gu, Biomimetic mineralization of nano-sized, needle-like hydroxyapatite with ultrahigh capacity for lysozyme adsorption, Mater. Sci. and Eng. C 68 (2016) 551–556.

DOI: 10.1016/j.msec.2016.06.021

Google Scholar

[3] A. Oyane, T. Ootsuka, K. Hayama, Y. Sogo, A. Ito, Enhanced immobilization of acidic proteins in the apatite layer via electrostatic interactions in a supersaturated calcium phosphate solution, Acta Biomaterialia 7 (2011) 2969–2976.

DOI: 10.1016/j.actbio.2011.04.012

Google Scholar

[4] M. Matsumoto, T. Miyake, H. Noshi, M. Kambara, K. Konishi, Zeta Potential Studies on the Adsorption of Proteins on a Synthetic Hydroxyapatite, Coll. And Surf. 40 (1989) 77-84.

DOI: 10.1016/0166-6622(89)80008-3

Google Scholar

[5] T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi, T. Yamamuro, Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W., J. Biomed. Mater. Res. 24 (1990) 721-734.

DOI: 10.1002/jbm.820240607

Google Scholar

[6] T. Kokubo, H. Takadama, How useful is SBF in predicting in vivo bone bioactivity?, Biomaterials 27 (2006) 2907-2915.

DOI: 10.1016/j.biomaterials.2006.01.017

Google Scholar

[7] H. Takadama, T. Kokubo, In vitro evaluation of bone bioactivity, in: T. Kokubo (Ed. ), Bioceramics and their clinical applications, Woodhead Publishing, Cambridge, 2008, pp.165-182.

DOI: 10.1533/9781845694227.1.165

Google Scholar

[8] T. Yao, M. Hibino, S. Yamaguchi H. Okada, Japanese Patent 5261712 (2013), U.S. Patent 8178066 (2012).

Google Scholar

[9] T. Yabutsuka, S. Tsuboi, M. Hibino, T. Yao, Fabrication of Encapsulated Ag Microsphere with Hydroxyapatite for Sustained-Release, Key Eng Mater. 361-363 (2008) 1199-1202.

DOI: 10.4028/www.scientific.net/kem.361-363.1199

Google Scholar

[10] S. Yamane, T. Yabustuka, M. Hibino, T. Yao, Fabrication of encapsulated silicagel microsphere with hydroxyapatite for sustained-release, Key Eng Mater. 396-398 (2009) 519-522.

DOI: 10.4028/www.scientific.net/kem.396-398.519

Google Scholar

[11] T. Matsunaga, T. Yabutsuka, S. Takai, T. Yao, Fabrication of Hydroxyapatite Microcapsules for Controlled Release of Hydrophobic Drug, Key Eng Mater. 720 (2017) 12-16.

DOI: 10.4028/www.scientific.net/kem.720.12

Google Scholar

[12] T. Yabutsuka, T. Yao, Fabrication of encapsulated magnetite microparticles with hydroxyapatite, Energy Procedia 9 (2011) 532-538.

Google Scholar

[13] S. Kumazawa, D. Hisashuku, T. Yabutsuka, T. Yao, Fabrication of Magnetic Hydroxyapatite Microcapsule for Protein Collection, Key Eng. Mater. 587 (2014) 160-164.

DOI: 10.4028/www.scientific.net/kem.587.160

Google Scholar

[14] T. Yabutsuka, S. Kumazawa, D. Hisashuku, H. Mizutani, K. Fukushima, S. Takai, T. Yao, Enzyme Immobilization by Using Apatite Microcapsules with Magnetic Properties, Key Eng. Mater, 696 (2016) 259-264.

DOI: 10.4028/www.scientific.net/kem.696.259

Google Scholar

[15] M. Bradford, A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem. 72 (1976) 248-254.

DOI: 10.1016/0003-2697(76)90527-3

Google Scholar

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