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Antifungal, Anti-Inflammatory and Cytotoxic Effects of Zingiber cassumunar Gel

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Abstract:

This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

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Periodical:

Key Engineering Materials (Volume 773)

Pages:

360-364

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.773.360

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Online since:

July 2018

Authors:

Sroisiri Thaweboon*, Boonyanit Thaweboon, Rattiporn Kaypetch

Keywords:

Antifungal, Anti-Inflammatory, Cytotoxicity, Zingiber cassumunar

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© 2018 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] C. B. Singh, N. Manglembi, N. Swapana, S. B. Chanu, Ethnobotany, Phytochemistry and Pharmacology of Zingiber cassumunar Roxb (Zingiberaceae), J. P. P 4 (2015) 1-6.

Google Scholar

[2] P. Pithayanukul, J. Tubprasert, M. Wuthi-Udomlert, In vitro antimicrobial activity of Zingiber cassumunar (Plai) oil and a 5% plai oil gel, Phytother. Res. 21 (2007) 164-169.

DOI: 10.1002/ptr.2048

Google Scholar

[3] S. Koontongkaew, L. Meesuk, V. Aupaphong V, T. D. Ayudhaya, O. Poachanukoon, Inhibitory effect of Zingiber cassumunar extracts on lipopolysaccharide-induced cyclooxygenase-2 and matrix metalloproteinase expression in human gingival fibroblasts, J. Periodont. 48 (2013).

DOI: 10.1111/jre.12033

Google Scholar

[4] I. B. Jantan, M. S. M. Yassin, C. B. Chin, L. L. Chen, N. L. Sim, Antifungal activity of the essential oils of nine Zingiberaceae species, Pharmaceut. Biol. 41 (2003) 392-397.

DOI: 10.1076/phbi.41.5.392.15941

Google Scholar

[5] J. K. Oberoi, C. Wattal, N. Goel, R. Raveendran, S. Datta, K. Prasad, Non-albicans Candida species in blood stream infections in a tertiary care hospital at New Delhi, India, Indian. J. Med. Res. 136 (2012) 997-1003.

Google Scholar

[6] E. Borenfreund, J. A. Puerner, A simple quantitative procedure using monolayer cultures for cytotoxicity assays, J. Tiss. Cult. Method. 1 (1984) 7-9.

Google Scholar

[7] International Organization for Standardization ISO Technical Report 7405—2008, Evaluation of biocompatibility of medical devices used in dentistry. ISO, Geneva, Switzerland (2008).

Google Scholar

[8] M. Merlos, L. A. Gómez, M. Giral, M. L. Vericat, J. García-Rafanell, J. Forn, Effects of PAF-antagonists in mouse ear oedema induced by several inflammatory agents, Br. J. Pharmacol. 104 (1991) 990-994.

DOI: 10.1111/j.1476-5381.1991.tb12538.x

Google Scholar

[9] R. Giwanon, S. Thubthimthed, U. Rerk-am, T. Sunthorntanasart: Antimicrobial activity of Terpinen-4-ol and sabinene, Thai. J. Pharm. Sci. 24 (2000) 27-30.

Google Scholar

[10] S. Nakamura, J. Iwami, H. Matsuda, H. Wakayama, Y. Pongpiriyadecha, M. Yoshikawa: Structires of new phenylbutanoids and nitric oxide production inhibitors from the rhizomes of Zingiber cassumunar, Chem. Pharm. Bull Tokyo). 57 (2009) 1267-1272.

DOI: 10.1248/cpb.57.1267

Google Scholar

[11] C. Singh, N. Manglembi, N. Swapana, S.B. Chanu: Ethanobotany, phytochemistry and pharmacology of Zingiber cassumunar Roxb. (Zingiberaceae), J. Pharmacog. Phyto. 4 (2015) 1-6.

Google Scholar

[12] U. Sukatta, P. Rugthaworn, P. Punjee, S. Chidchenchey, V. Keeratinijakal, Chemical composition and physical properties of oil from plai (Zingiber cassumunar Roxb. ) obtained by hydro distillation and hexane extraction, Kasetsart. J, (Nat Sci). 43 (2009).

Google Scholar

[13] J. Sikkema, J. A. de Bon, B. Pollman, Mechanisms of membrane toxicity of hydrocarbons, Microbiol. Rev. 59 (1995) 201-222.

DOI: 10.1128/mr.59.2.201-222.1995

Google Scholar

[14] M. Gabor: The mouse ear as a model for cutaneous irritation, J. Toxico.-Cut. & Ocular. Toxicol. 21 (2002) 191-202.

Google Scholar

[15] E. De Bernardis, G. Leonardi, A. Caruso, V.M.C. Cutuli, M. Amico-Roxas: Protective effects of papaverine salicylate in mouse ear dermatitis and PAF induced rat paw oedema, Agents. Actions. 42 (1994) 29–33.

DOI: 10.1007/bf02014296

Google Scholar

[16] P. H. Hart, C. Brand, C. F. Carson, T. V. Riley, R. H. Prager, J. J. Finlay-Jones, Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes, Inflamm. Res. 49 (2000).

DOI: 10.1007/s000110050639

Google Scholar

[17] J. Valente, M. Zuzarte, M. J. Gonçalves, M. C. Lopes, C. Cavaleiro, L. Salgueiro, et al., Antifungal, antioxidant and anti-inflammatory activities of Oenanthe crocata L. essential oil, Food. Chem. Toxicol. 62 (2013) 349-354.

DOI: 10.1016/j.fct.2013.08.083

Google Scholar

[18] W. Chaiyana, S. Anuchapreeda, P. Leelapornpisid, R. Phongpradist, H. Viernstein, M. Mueller, Development of microemulsion delivery system of essential oil from Zingiber cassumunar Roxb. rhizome for improvement of stability and anti-inflammatory activity, AAPS. Pharm. Sci. Tech. 8 (2016).

DOI: 10.1208/s12249-016-0603-2

Google Scholar

[19] S. Koontongkaew, O. Poachanukoon, S. Sireeratawong, T. Dechatiwongse Na Ayudhya, P. Khonsung, K. Jaijoy et al: Safety evaluation of Zingiber cassumunar Roxb. rhizome extract: acute and chronic toxicity studies in rats, Int. Sch. Res. Notices 16 (2014).

DOI: 10.1155/2014/632608

Google Scholar

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