Using Confocal Laser Scanning Microscopy to Study an Ambers, a New Approach

Abstract:

Currently, in the scientific literature, there is no a sole opinion on mechanisms governing phenomenon of blue amber. In specimens sampled in Dominican Republic it is caused by presence of perylene; whereas, specimens sampled in northern Spain this has not been confirmed. We have obtained specimens from a third, earlier not studied, source of blue amber in the Far East of Russia that was described in 2015. This sample was investigated with a laser confocal microscopy and a laser bleaching studies. We found fluorescence pattern that is typical for perylene and bleaching showed rapid diffusion that is consistent with the perylene hypothesis. Moreover we demonstrate that confocal laser scanning microscopy could be successfully used for rapid search of the organisms in the dark part of the amber samples.

• Periodical: Key Engineering Materials (Volume 806)
• Pages: 197-202
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.806.197
• Online since: June 2019
• Authors: Alexander Mikhailovich Zakharenko*, Igor Yurevich Chekryzhov, Kirill Sergeevich Golokhvast
• Keywords: Amber, Blue Amber, CLSM, Perylene, Voznovo Formation

References

[1] M. A. Iturralde-Vinent, Geology of the amber-bearing deposits of the Greater Antilles. Caribbean Journal of Science 37 (2001) 141-167.

[2] V. Bellani, E. Giulotto, L. Linati, D. Sacchi. Origin of the blue fluorescence in Dominican amber.Journal of Applied Physics 97 (2005) 2.

DOI: 10.1063/1.1829395

[3] C. Menor-Salvan et al., Terpenoids in extracts of Lower Cretaceous ambers from the Basque-Cantabrian Basin (El Soplao, Cantabria, Spain): Paleochemotaxonomic aspects. Organic Geochemistry 41 (2010) 1089-1103.

DOI: 10.1016/j.orggeochem.2010.06.013

[4] M. Najarro, E. Penalver, I. Rosales, R. Perez-De La Fuente, V. Daviero-Gomez, B. Gomez. nusual concentration of Early Albian arthropod-bearing amber in the Basque-Cantabrian Basin (El Soplao, Cantabria, Northern Spain): Palaeoenvironmental and palaeobiological implications. Geologica Acta 7 (2009) 363-387.

DOI: 10.1111/j.1755-6724.2010.00258.x

[5] I.Y. Chelayzhov, V.P. Nechaev, V.V. Kononov. Blue-fluorescing amber from Cenozoic lignite, eastern Sikhote-Alin, Far East Russia: Preliminary results. International Journal of Coal Geology 132 (2014) 6-12.

DOI: 10.1016/j.coal.2014.07.013

[6] B.I. Pavlyutkin, I.Y. Chekryzhov, T.I. Petrenko. The Voznovo Formation: The reflection of the early Oligocene stage in the geological history of East Sikhote-Alin. Russian Journal of Pacific Geology 5 (2011) 47-63.

DOI: 10.1134/s1819714011010052

[7] Y. Otofuji, T. Matsuda, T. Itaya, T. Shibata, M. Matsumoto, T. Yamamoto. Late cretaceous to early paleogene paleomagnetic results from Sikhote-Alin, Far-Eastern Russia - implications for deformation of East-Asia. Earth and Planetary Science Letters 130 (1995) 95-108.

DOI: 10.1016/0012-821x(94)00254-v

[8] S. Okamura, Y.A. Martynov, K. Furuyama, K. Nagao. K-Ar ages of the basaltic rocks from Far East Russia: Constraints on the tectono-magmatism associated with the Japan Sea opening. Island Arc 7 (1998) 271-282.

DOI: 10.1046/j.1440-1738.1998.00174.x