Application of Plant Extracts as a Preservative in an Aqueous Gel Formulation

Abstract:

This research aims to study the antimicrobial activities of five selected plants, which were cinnamon bark, betel leaves, licorice, ginger, and galangal and select the most effective herbal extracts to be evaluated for their preservative efficacies in aqueous gel formulation. The sequential solvent extraction using hexane and 95% ethanol was used to extract bioactive constituents in the studied plants. After completely evaporated the solvents, the crude extracts were tested for the antimicrobial activities against S.aureus TISTR 1466 (ATCC 6538), P.aeruginosa TISTR 781 (ATCC 9027), E.coli ATCC 7839, C.albicans TISTR 5779 (ATCC 10201) and A.brasiliensis DMST 15538 (ATCC 16404) by agar well diffusion method. The result showed that the cinnamon bark extract macerated in hexane could inhibit the growth of all studied micro-organisms and was more effective than betel leaves, ginger, galangal and licorice extracts, while its 95% ethanolic extracts obviously showed lower antimicrobial activities. The MIC and MBC or MFC of cinnamon bark extract against the testing micro-organisms were in a range of 0.039-0.312 mg/mL and 0.156-1.250 mg/mL/0.078-0.625 mg/mL, respectively. The effective doses of hexane extract of cinnamon bark attempted to be tested as preservative in aqueous gel formulation were 0.3%, 1.25% and 2.5% by weight, which were equal to MIC, 4MIC and 8MIC, respectively. The challenge tests according to ISO 11930 were carried out to determine the preservative efficacy. The results revealed that 1.25% and 2.5% of cinnamon bark extract satisfied the criteria B for bacterial strains and the criteria A for yeast and mold. The LC-MS data confirmed that the two main constituents in hexane extract of cinnamon bark were coumarin and cinnamaldehyde that might play a role in antimicrobial activity.

• Periodical: Key Engineering Materials (Volume 859)
• Pages: 172-180
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.859.172
• Online since: August 2020
• Authors: Punnidtha Bupphatanarat, Worrapannee Powtongsook, Chawalinee Asawahame, Paveena Wongtrakul*
• Keywords: Antimicrobial Activity, Challenge Tests, Plants, Preservatives

References

[1] M. Dreger, K. Wielgus, Application of essential oils as natural cosmetic preservatives. Herba Polonica. 59 (2013) 142-155.

DOI: 10.2478/hepo-2013-0030

[2] N. Y. O. Muyima, G. Zulu, T. Bhengu, D. Popplewell, The potential application of some novel essential oils as natural cosmetic preservatives in an aqueous cream formulation. Flavour Fragr. J. 17 (2002) 258–266.

DOI: 10.1002/ffj.1093

[3] I. Manou, L. Bouillard, M.J. Devleeschouwer, A.O. Barel, Evaluation of the preservative properties of Thymus vulgaris essential oil in topically applied formulations under a challenge test. J. Appl. Microbiol. 84 (1998) 368–376.

DOI: 10.1046/j.1365-2672.1998.00353.x

[4] A. M. Maccioni, C. Anchisi, A. Sannay, C. Sarduy S. Dess'y, Preservative systems containing essential oils in cosmetic product. Int J Cosmet. Sci. 24 (2002) 53-59.

DOI: 10.1046/j.0412-5463.2001.00113.x

[5] A. Nostro, M.A. Cannatelli, I. Morelli, A.D. Musolino, F. Scuderi, F. Pizzimenti, V. Alonzo, Efficiency of Calamintha officinalis essential oil as preservative in two topical product types. J. Appl. Microbiol. 97 (2004) 395–401.

DOI: 10.1111/j.1365-2672.2004.02319.x

[6] A. Kunicka-Styczyn´ska, M. Sikora, D. Kalemba, Antimicrobial activity of lavender, tea tree and lemon oils in cosmetic preservative systems. J. Appl. Microbiol. 107 (2009) 1903–(1911).

DOI: 10.1111/j.1365-2672.2009.04372.x

[7] A. Herman, A.P. Herman, B.W. Domagalska, A. Młynarczyk, Essential Oils and Herbal Extracts as Antimicrobial Agents in Cosmetic Emulsion. Ind. J Microbiol. 53(2013) 232–237.

DOI: 10.1007/s12088-012-0329-0

[8] J.S. Yang, A Study on the Cosmetic Preservative Effects of Cornus officinalis seed Extracts. J. of Korean Oil Chemists' Soc. 33 (2016) 333-334.

DOI: 10.12925/jkocs.2016.33.2.333

[9] S. Boukhira, M. Balouiri, E.L. Mansouri, E.Y. Ae, M. Bouarfa, S. Lebtar, A. Ouhammou, D. Bousta, Development of Natural Preservative from Silene vulgaris Extract in Topical Formulation under a Challenge Test and its Stability Study. J App Pharm Sci, 7 (2017) 142-148.

DOI: 10.7324/japs.2017.70421

[10] W. Siegert, ISO 11930 – A Comparison to other Methods to Evaluate the Efficacy of Antimicrobial Preservation. Int. J. Appl. Sci. 138 (2012) 44-53.

[11] Supriatno and Lelifajri, Effect of Sequential Extraction on Total Phenolic Content (TPC) and Antioxidant Activities (AA) of Luffa acutangula Linnaeus Dried Pulps. AIP Conference Proceedings 2002, 020062 (2018).

DOI: 10.1063/1.5050158

[12] F. Karahana, C. Avsarb, I.I Ozyigitc, I. Berber, Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats. Biotechnol. Biotechnol. Equip. 30 (2016) 797-804.

DOI: 10.1080/13102818.2016.1179590

[13] M. Mahboubi, Zingiber officinale Rosc. essential oil, a review on its composition and bioactivity. Clinical Phytoscience 5 (2019) 1-12.

DOI: 10.1186/s40816-018-0097-4

[14] Y. Liang, Y. Li, A. Sun, X. Liu, Chemical compound identification and antibacterial activity evaluation of cinnamon extracts obtained by subcritical n‐butane and ethanol extraction. Food Sci Nutr. 7 (2019) 2186–2193.

DOI: 10.1002/fsn3.1065

[15] G. Singh, S. Maurya, M.P. deLampasona, C.A.N. Catalan, A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents. Food Chem Toxicol. 45 (2007) 1650–1661.

DOI: 10.1016/j.fct.2007.02.031

[16] C. Gupta, A.P. Garg, R.C. Uniyal, A. Kumari, Comparative analysis of the antimicrobial activity of cinnamon oil and cinnamon extract on some food-borne microbes. Afr. J. Microbiol. Res. 2(2008) 247-251.

[17] I. Utchariyakiat, S. Surassmo, M. Jaturanpinyo, P. Khuntayaporn, P., M.T. Chomnawang, Efficacy of cinnamon bark oil and cinnamaldehyde on anti-multidrug resistant Pseudomonas aeruginosa and the synergistic effects in combination with other antimicrobial agents. BMC Complement Altern Med.16 (2016) 1-7.

DOI: 10.1186/s12906-016-1134-9

[18] N.N. Kasim, S.N.A.S. Ismail, N.D. Masdar, F.A. Hamid, W.I. Nawawi, Extraction and Potential of Cinnamon Essential Oil towards Repellency and Insecticidal Activity. Int. J. of Sci. and Research Publications, 4 (2014) 1-6.

[19] N.G. Vasconcelos, J. Croda, S. Simionatto, Antibacterial mechanisms of cinnamon and its constituents: A review. Microb Pathog. 120 (2018) 198-203.

DOI: 10.1016/j.micpath.2018.04.036

[20] A. Nostro, M.A. Cannatelli, I. Morelli, P.L. Cioni, A. Bader, A. Marino, V. Alonzo, Preservative properties of Calamintha officinalis essential oil with and without EDTA. Lett. Appl. Microbiol. 35 (2002) 385–389.

DOI: 10.1046/j.1472-765x.2002.01216.x

[21] Y.E. Atki, F.E. Kamari, A. Taroq, K. Nayme, M. Timinouni, B. Lyoussi, A. Abdellaoui, Antibacterial activity of cinnamon essential oils and their synergistic potential with antibiotics. J. Adv. Pharm. Technol. Res. 10 (2019) 63-67.

DOI: 10.4103/japtr.japtr_366_18