Physical, Chemical, and Microbiological Stability of Mucuna pruriens Effervescent Powders and Suspension

Abstract:

Mucuna pruriens is a tropical leguminous plant containing L-dopa which is the main active ingredient for Parkinson’s disease therapy. The objective of this study was to investigate the physical, chemical, and microbiological stability of M. pruriens seed extract effervescent powders and suspension. The effervescent powders and suspension were prepared using freeze dried M. pruriens seed extract and were stored at 4 °C, 30 °C, and 45 °C. Physical stability was evaluated by observing appearance and determining rheological behavior. Chemical stability was evaluated by HPLC assay of L-dopa content in the formulation. Bacterial, fungal and mold contaminations were assessed by 3M^TM Petrifilm^TM count plates. Both formulations showed good physical stability. The degradations of L-dopa in effervescent powders and suspension were fitted to pseudo-zero order and second order kinetic models, respectively. M. pruriens seed extract effervescent powders was found to have the longest half-life, about 112 days, when it was stored at 4 °C. The results suggested that M. pruriens seed extract should be formulated in effervescent powders and stored at 4°C to prevent physical and chemical degradation.

• Periodical: Key Engineering Materials (Volume 859)
• Pages: 145-150
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.859.145
• Online since: August 2020
• Authors: Chuda Chittasupho*, Sarin Tadtong, Suwanna Vorarat, Narisa Kamkaen
• Keywords: Contamination, Effervescent Powders, L-Dopa, Mucuna pruriens, Stability, Suspension

References

[1] A.B. Vaidya, T.G. Rajagopalan, N.A. Mankodi, D.S. Antarkar, P.S. Tathed, A.V. Purohit, N.H. Wadia, Treatment of Parkinson's disease with the cowhage plant-Mucuna pruriens Bak. Neurol India. 26 (1978) 171-176.

[2] G. Nikolova, Y. Karamalakova, and V. Gadjeva, Reducing oxidative toxicity of L-dopa in combination with two different antioxidants: an essential oil isolated from Rosa Damascena Mill., and vitamin C. Toxicol Rep. 6 (2019) 267-271.

DOI: 10.1016/j.toxrep.2019.03.006

[3] R. Katzenschlager, A. Evans, A. Manson, P.N. Patsalos, N. Ratnaraj, H. Watt, L. Timmermann, R. Van der Giessen, A.J. Lees, Mucuna pruriens in Parkinson's disease: a double blind clinical and pharmacological study. J Neurol Neurosurg Psychiatry. 75 (2004) 1672-1627.

DOI: 10.1136/jnnp.2003.028761

[4] E.J. Pappert, C. Buhrfiend, J.W. Lipton, P.M. Carvey, G.T. Stebbins, C.G. Goetz, Levodopa stability in solution: time course, environmental effects, and practical recommendations for clinical use. Mov Disord. 11 (1996) 24-26.

DOI: 10.1002/mds.870110106

[5] J.J. Lopez Lozano, R. Moreno Cano, Preparation of a levodopa/carbidopa solution in ascorbic acid (citridopa) and chromatographic and electrochemical assessment of its stability over 24 hours. Neurologia. 10, (1995) 155-158.

[6] B.A., Moreira, F.d. Oliveira, A.J.R. Damasceno, Analysis of suspension sedimentation in fluids with rheological shear-thinning properties and thixotropic effects. Powder Technology. 308 (2017) 290-297.

DOI: 10.1016/j.powtec.2016.12.034

[7] A. Fulias, G. Vlase, D. Cîrcioban, I. Ledeti,  L. Stelea, T. Vlase, A. Caunii, Comparative Stability of Levodopa Under Thermal Stress in both Oxidative and Inert Media. Revista de Chimie, 17 (2016) 2648-2650.

[8] Y.Z. Zhou, R.G. Alanv, V. Chuang, J. Wen. Studies of the Rate Constant of L-DOPA Oxidation and Decarboxylation by HPLC. Chromatographia, 75 (2012) 597-606.

DOI: 10.1007/s10337-012-2229-1