A Preparation Method of β-Carotene Reference Standard from β-Carotene Food Additive Based on High Speed Countercurrent Chromatography (HSCCC)

Abstract:

The paper provided a simple method for preparing the β-carotene reference standard from a β-carotene food additive (96%) based on high speed countercurrent chromatography (HSCCC). The obtained material was qualitatively detected by IR, NMR, MS, the results showed the material was purified β-carotene, the purity of obtained β-carotene reference standard can be as high as 99%. The prepared reference standard can be used for quality control of related health food. The provided method is simple, universal, cheap, and the sample preparation can be enlarged to g grade.

• Periodical: Key Engineering Materials (Volume 871)
• Pages: 293-299
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.871.293
• Online since: January 2021
• Authors: Lan Tao, Zhi Ming Wang, Shu Huan Lu, Liu Liu Xu, Mu Zi Li, Xiang Yu Li*
• Keywords: Food Additive, Health Food, High Speed Countercurrent Chromatography (HSCCC), Reference Standard, β-Carotene

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