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HomeKey Engineering MaterialsKey Engineering Materials Vol. 884Cheap Cellulase Production by Aspergillus sp. VTM1...

Cheap Cellulase Production by Aspergillus sp. VTM1 Through Solid State Fermentation of Coffee Pulp Waste

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Abstract:

Coffee pulp biomass waste can easily be found anywhere in Indonesia, considering it is the fourth world's largest coffee exporter. The utilization of coffee pulp is very limited and is categorized as a source of pollutants in water bodies and soils. In contrast, coffee pulp waste is very potential because 63% of the main compound is cellulose. Microbial utilization of this waste for enzyme production purposes, especially cellulase, is a breakthrough that may lead to reduce production costs. Initial investigations showed that Aspergillus sp. VTM1 through solid-state fermentation (SSF) could produce cellulases. Optimal cellulase could be produced if 10 g coffee pulp with 10% moisture is inoculated using 10⁸ spores/mL of Aspergillus sp. VTM1 for 48 hours at 30 °C. Hydrolysis of 1% carboxymethyl cellulose (CMC) substrate in 50 mM acetate buffer pH 5 by this cellulase showed that the enzyme activity reached up to 1.18 U/mL. The optimum pH of the enzyme was 5 and stable at 3-3.5 and 4-7. The success of the first step of this investigation will be a cheap way of producing cellulases.

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Symposium of Materials Science and Chemistry III

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Periodical:

Key Engineering Materials (Volume 884)

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159-164

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.884.159

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Online since:

May 2021

Authors:

Reni Rusdianti, Azizah Azizah, Esti Utarti, Hidayat Teguh Wiyono, Kahar Muzakhar*

Keywords:

Aspergillus Sp. VTM1, Cellulase, Coffee Pulp, Production, Solid-State Fermentation

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© 2021 Trans Tech Publications Ltd. All Rights Reserved

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[1] R.C. Kuhad, R. Gupta, A. Singh, Microbial cellulases and their industrial applications, Enzyme Res. 2011 (2011) 280696.

[2] D.S. Chahal, Solid-state fermentation with Trichoderma reesei for cellulase production, Appl. Environ. Microbiol. 49 (1) (1985) 205-210.

DOI: 10.1128/aem.49.1.205-210.1985

[3] X-Z. Zhang, Y-H.P. Zhang, Cellulases: characteristics, sources, production, and applications, in: S-T. Yang, H.A. El-Enshasy, N. Thongcul (Eds.), Bioprocessing Technologies in Biorefinery for Sustainable Production of Fuels, Chemicals, and Polymers, John Wiley & Sons, Inc., New Jersey, 2013, pp.131-146.

DOI: 10.1002/9781118642047.ch8

[4] K. Muzakhar, Sutoyo, A.B. Saragih, Phosphate solubilizing bacteria adaptive to vinasse, J. Math. Fundam. Sci. 47 (2) (2015) 219-225.

DOI: 10.5614/j.math.fund.sci.2015.47.2.8

[5] M. Sohail, R. Siddiqi, A. Ahmad, S.A. Khan, Cellulase production from Aspergillus niger MS82: effect of temperature and pH, N. Biotechnol. 25 (6) (2009) 437-441.

DOI: 10.1016/j.nbt.2009.02.002

[6] S. Mrudula, R. Murugammal, Production of cellulase by Aspergillus niger under submerged and solid-state fermentation using coir waste as a substrate, Braz. J. Microbiol. 42 (2011) 1119-1127.

DOI: 10.1590/s1517-83822011000300033

[7] K. Muzakhar, A Consortium of Three Enzymes: xylanase, arabinofuranosidase, and cellulase from Aspergillus sp. which liquefied coffee pulp wastes, IOP Conf. Ser.: Mater. Sci. Eng. 546 (2) (2019) 022013.

DOI: 10.1088/1757-899x/546/2/022013

[8] W. Yuniar, Skrining dan identifikasi kapang selulolitik pada proses vermikomposting tandan kosong kelapa sawit, Undergraduate Thesis, Universitas Jember, Jember, (2013).

[9] A. Pandey, C.R. Soccol, D. Mitchell, New developments in solid state fermentation: I- bioprocesses and products, Process Biochem. 35 (10) (2000) 1153-1169.

DOI: 10.1016/s0032-9592(00)00152-7

[10] Information on http://www.ico.org.

[11] S. Roussos, M.de los A. Aquiáhuatl, M.del R. Trejo-Hernández, I.G. Perraud, E. Favela, M. Ramakrishna, M. Raimbault, G. Viniegra-González, Biotechnological management of coffee pulp - isolation, screening, characterization, selection of caffeine-degrading fungi and natural microflora present in coffee pulp and husk, Appl. Microbiol. Biotechnol. 42 (1995) 756-762.

DOI: 10.1007/bf00171958

[12] G. Corro, L. Paniagua, U. Pal, F. Bañuelos, M. Rosas, Generation of biogas from coffee- pulp and cow-dung co-digestion: Infrared studies of postcombustion emissions, Energy Convers. Manag. (2013) 471-481.

DOI: 10.1016/j.enconman.2013.07.017

[13] L.G.A. Ong, S. Abd-Aziz, S. Noraini, M.I.A. Karim, M.A. Hassan, Enzyme production and profile by Aspergillus niger during solid substrate fermentation using palm kernel cake as substrate, Appl. Biochem. Biotechnol. 118 (1-3) (2004) 73-79.

DOI: 10.1385/abab:118:1-3:073

[14] S. Ubaidillah, K. Muzakhar, Sugar-rich hydrolyzates from coffee pulp waste which produced under solid state fermentation by Pestalotiosis sp. VM9 and Aspergillus sp. VTM5, and its efficiency as medium for single cell protein Saccharomyces cerevisiae, IOP Conf. Ser.: Mater. Sci. Eng. 546 (6) (2019) 062033.

DOI: 10.1088/1757-899x/546/6/062033

[15] T.M. Wood, K.M. Bhat, Methods for measuring cellulase activities, Methods Enzymol. 160 (1988) 87-112.

[16] K. Selvam, M. Govarthanan, S.K. Kannan, M. Govindharaju, B. Senthilkumar, T. Selvankumar, A. Sengottaiyan, Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC, RSC Adv. 4 (25) (2014) 13045-13051.

DOI: 10.1039/c4ra00066h

[17] M. Narra, G. Dixit, J. Divecha, D. Madamwa, A.R. Shah, Production of cellulases by solid state fermentation with Aspergillus terreus and enzymatic hydrolysis of mild alkali-treated rice straw, Bioresour. Technol. 121 (2012) 355-361.

DOI: 10.1016/j.biortech.2012.05.140

[18] M. Raimbault, D.A. Alazard, Culture method to study fungal growth in solid fermentation, Eur. J. Appl. Microbiol. Biotechnol. 9 (1980) 199-209.

DOI: 10.1007/bf00504486

[19] F. Deschamps, C. Giuliano, M. Asther, M.C. Huet, S. Roussos, Cellulase production by Trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions, Biotechnol. Bioeng. 27 (9) (1985) 1385-1388.

DOI: 10.1002/bit.260270917

[20] S.J.B. Duff, W.D. Murrayh, Bioconversion of forest products industry waste cellulosics of fuel ethanol : a review, Bioresour. Technol. 55 (1) (1996) 1-33.

DOI: 10.1016/0960-8524(95)00122-0

[21] N.M. Mubarak, J.R. Wong, K.W. Tan, J.N. Sahu, E.C. Abdullah, N.S. Jayakumar, P. Ganesan, Immobilization of cellulase enzyme on functionalized multiwall carbon nanotubes, J. Mol. Catal. B: Enzym. 107 (2014) 124-131.

DOI: 10.1016/j.molcatb.2014.06.002

[22] D. Liu, R. Zhang, X. Yang, H. Wu, D. Xu, Z. Tang, Q. Shen, Thermostable cellulase production of Aspergillus fumigatus Z5 under solid- state fermentation and its application in degradation of agricultural wastes, Int. Biodeterior. Biodegrad. 65 (5) (2011) 717-725.

DOI: 10.1016/j.ibiod.2011.04.005

[23] S.P. Gautam, P.S. Bundela, A.K. Pandey, J. Khan, M.K. Awasthi, S. Sarsaiya, Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi, Biotechnol. Res. Int. 2011 (2011) 810425.

DOI: 10.4061/2011/810425