Paper Titles

Extraction Behavior of Trivalent Rare Earth Metal Ions with Diphosphonic Acid Type Extraction Reagent
p.133

Conversion of Nyamplung Oil (Calophyllum inophyllum L.) into Liquid Fuel by Hydrocracking Process Using NiMo/γ-Al₂O₃ Bimetallic Based Catalyst
p.140

Performance of Ni-Cu/HZSM-5 Catalyst in Hydrocracking Process to Produce Biofuel from Cerbera manghas Oil
p.149

Cheap Cellulase Production by Aspergillus sp. VTM1 Through Solid State Fermentation of Coffee Pulp Waste
p.159

Pectinase Production by Using Coffee Pulp Substrate as Carbon and Nitrogen Source
p.165

The Effect of Sorghum Varieties (Sorghum Bicolor (L.) Moench) and Protein Levels on Chemical Composition and In Vitro Digestibility of Fermented Complete Feed
p.171

Modification Level of Polyethylene Glycol on In Vitro Gas Production of Feedstuffs
p.178

The Effect of Sorghum Varieties on Digestibility and Nitrogen Balance of Complete Feed in Goats
p.184

Evaluation of Functional Feed in Total Mixed Ration (TMR) with High Protein Content and Anthelmintic Agents Towards Performance and Anti-Parasitic Effect in Sheep
p.191

HomeKey Engineering MaterialsKey Engineering Materials Vol. 884Pectinase Production by Using Coffee Pulp...

Pectinase Production by Using Coffee Pulp Substrate as Carbon and Nitrogen Source

Article Preview

Abstract:

About thirty-five percent of coffee pulp waste is pectin. It may potentially be a source to be used in the bioprocessing industry. For example, it can be used as a substrate to produce pectinase from microorganisms under solid-state fermentation (SSF). In this investigation, an isolated fungus VTM4 with density 10⁷ spores/mL was grown on coffee pulp medium-based, and after 0-168 hours incubation at 30 °C, pectinase activity was detected. The activity was measured based on reducing sugar released by crude pectinase against 0.5% alkali extract pectin substrate in 20 mM buffer acetate pH 5. The highest reducing sugar produced was 223.34 µg/mL after 72 hours of incubation at 30 °C. The optimum pH on enzyme activity was 4 with the maximum activity 0.747 U/mL and was stable (more than 80%) at a pH range of 4-5.5. The results revealed that the coffee substrate could be utilized as a carbon and nitrogen source to produce pectinase. Further research on purification and characterization of the enzyme to improve pectinase yield production was needed.

You might also be interested in these eBooks

Symposium of Materials Science and Chemistry III

Info:

Periodical:

Key Engineering Materials (Volume 884)

Pages:

165-170

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.884.165

Citation:

Cite this paper

Online since:

May 2021

Authors:

Okta Novalia Gasani, Azizah Azizah, Siswanto Siswanto, Rudju Winarsa, Kahar Muzakhar*

Keywords:

Coffee Pulp, Optimization of pH, Pectinase, pH Stability, Solid State Fermentation

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2021 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] ICO, Coffee production by exporting countries, International Coffee Organization, London, (2019).

[2] J.S. da Silveira, N. Durand, S. Lacour, M-P. Belleville, A. Perez, G. Loiseau, M. Dornier, Solid-state fermentation as a sustainable method for coffee pulp treatment and production of an extract rich in chlorogenic acids, Food Bioprod. Process. 115 (2019) 175-184.

DOI: 10.1016/j.fbp.2019.04.001

[3] D. Silva, K. Tokuioshi, E.D.S. Martins, R. da Silva, E. Gomes, Production of pectinase by solid-state fermentation with Penicillium viridicatum RFC3, Process Biochem. 40 (8) (2005) 2885-2889.

DOI: 10.1016/j.procbio.2005.01.008

[4] K. Muzakhar, A consortium of three enzymes : xylnase, arabinofuranosidase and cellulase from Aspergillus sp. which liquefied pulp waste, IOP Conf. Ser.: Mater. Sci. Eng. 546 (2) (2019) 022013.

DOI: 10.1088/1757-899x/546/2/022013

[5] E.S. Martins, D. Silva, R. da Silva, E. Gomes, Solid state production of thermostable pectinases from thermophilic Thermoascus aurantiacus, Process Biochem. 37 (9) (2002) 949-954.

DOI: 10.1016/s0032-9592(01)00300-4

[6] B. Rivas, A. Torrado, P. Torre, A. Converti, J.M. Domínguez, Submerged citric acid frmentation on orange peel autohydrolysate, J. Agric. Food Chem. 56 (7) (2008) 2380-2387.

DOI: 10.1021/jf073388r

[7] U. Hasanah, M. Setyowati, Edwarsyah, R. Efendi, E. Safitri, R. Idroes, L.Y. Heng, N.D. Sani, Isolation of Pectin from coffee pulp Arabica Gayo for the development of matrices membrane, IOP Conf: Ser.: Mater. Sci. Eng. 523 (2019) 012014.

DOI: 10.1088/1757-899x/523/1/012014

[8] K. Roy, S. Dey, Md.K. Uddin, R. Barua, Md.T. Hossain, Extracellular pectinase from a novel bacterium Chryseobacterium indologenes strain SD and its application in fruit juice clarification, Enzyme Res. 2018 (2018) 3859752.

DOI: 10.1155/2018/3859752

[9] D.R. Kashyap, P.K. Vohra, S. Chopra, R. Tewari, Applications of pectinases in the commercial sector: a review, Bioresour. Technol. 77 (3) (2001) 215-227.

DOI: 10.1016/s0960-8524(00)00118-8

[10] G. Aguilar, C. Huitrón, Conidial and mycelial-bound exo-pectinase of Aspergillus sp., FEMS Microbiol. Lett. 108 (2) (1993) 127-132.

DOI: 10.1111/j.1574-6968.1993.tb06087.x

[11] K. Muzakhar, Sutoyo, A.B. Saragih, Phosphate Solubilizing Bacteria Adaptive to Vinnasse, J. Math. Fundam. Sci. 47 (2) (2015) 219-225.

DOI: 10.5614/j.math.fund.sci.2015.47.2.8

[12] L. Ikasari, D.A. Mitchell, Protease production by Rhizopus oliigosporus in solid-state fermentation, World J. Microbiol. Biotechnol.10 (1994) 320-324.

DOI: 10.1007/bf00414872

[13] K. Muzakhar, R. Winarsa, Activity of α-L-Rhamnosidase proced by Aspergilluus niger during solid state fermentation of coffee pulp wastes, J. Biodjati. 4 (1) (2019) 105-111.

DOI: 10.15575/biodjati.v4i1.4411

[14] S. Ubaidillah, K. Muzakhar, Sugar-rich hydrolyzates from coffee pulp waste which produced under solid state fermentation by Pestalotiosis sp. VM9 and Aspergillus sp. VTM 5, and its effenciency as medium for single cell protein Saccharomyces cerevisiae, IOP Conf. Ser.: Mater. Sci. Eng. 546 (6) (2019) 062033.

DOI: 10.1088/1757-899x/546/6/062033

[15] K. Muzakhar, Masruroh, Siswoyo, R. Winarsa, Sutoyo, Sugar-rich hydrolysates of palm oil empty fruit bunch production through two step solid state fermentations and its conversion to ethanol, Adv. Sci. Lett. 23 (3) (2017) 2533-2535.

DOI: 10.1166/asl.2017.8684

[16] Z.W. Strain, G. Coral, Some Properties of crude carboxymethyl cellulase of Aspergillus niger Z10 wild-type strain, Turk. J. Biol. 26 (4) (2002) 209-213.

[17] V.H. Vu, T.A. Pham, K. Kim, improvement of fungal cellulase production by mutation and optimization of soolid state fermentation, Mycobiology. 39 (1) (2011) 20-25.

DOI: 10.4489/myco.2011.39.1.020

[18] K. Mojsov, Experimental investigations of submerged fermentation and synthesis of pectinolytic enzymes by Aspergillus Niger: effect of inoculum size and age of spores, Appl. Technol. Innov. 2 (2) (2010) 40-46.

DOI: 10.15208/ati.2010.13

[19] S.C. Sati, S. Bisht, Utilization of various carbon sources for the growth of waterborne conidial fungi, Mycologia. 98 (5) (2006) 678-681.

DOI: 10.3852/mycologia.98.5.678

[20] T. Sander, N. Farke, C. Diehl, M. Kuntz, T. Glatter, H. Link, Allosteric feedback inhibiton enables robust amino acid biosynthesis in E.coli by enforcing enzyme overabundance, Cell Syst. 8 (1) (2019). 66-7.

DOI: 10.1016/j.cels.2018.12.005

[21] D.B. Pedrolli, A.C. Monteiro, E. Gomes, E.C. Carmona, Pectin and pectinase: production, characterization and industrial application of microbial pectinolytic enzymes, Open Biotechnol. J. 3 (1) (2009) 9-18.

DOI: 10.2174/1874070700903010009