Development of Oral Cleansing Products Containing Green Tea and Centella asiatica Extracts

Abstract:

“Mild” oral care products are needed by some groups of people, such as radiotherapy patients. This study developed oral cleansing products containing green tea (Camelia sinensis (L.) Kuntze) extracts with antibacterial activities and Centella asiatica (L.) Urb. extracts with a wound healing activity. We found that 3.13 mg/ml of green tea extract inhibited both Streptococcus mutans and Candida albicans, which can cause dental caries and oral candidiasis, respectively. Also, a 16 μg/ml C. asiatica extract promoted fibroblast migration in a wound healing study. The products were developed in 2 dosage forms, i.e. a solution and an impregnated gauze, which were isotonic, alcohol and volatile oil free formulations to prevent irritation and burning sensations. The products showed acceptable physical stability after testing under stressed conditions with post-test retention of antimicrobial activity.

• Periodical: Key Engineering Materials (Volume 901)
• Pages: 48-54
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.901.48
• Online since: October 2021
• Authors: Nattaporn Amornopparattanakul*, Neti Waranuch, Supaporn Lamlertthon, Wudtichai Wisuitiprot, Kornkanok Ingkaninan
• Keywords: Antimicrobial, Centella asiatica, Green Tea, Oral Cleansing Products, Wound Healing

References

[1] E.d.S. Tolentino, B.S. Centurion, L.H.C. Ferreira, A.P.d. Souza, J.H. Damante, I.R.F. Rubira-Bullen, Oral adverse effects of head and neck radiotherapy: literature review and suggestion of a clinical oral care guideline for irradiated patients, J. Appl. Oral Sci. 19(5) (2011) 448-454.

DOI: 10.1590/s1678-77572011000500003

[2] S. Patil, R.S. Rao, B. Majumdar, S. Anil, Clinical appearance of oral Candida infection and therapeutic strategies, Front. Microbiol. 6 (1391) (2015).

DOI: 10.3389/fmicb.2015.01391

[3] A. Chatterjee, M. Saluja, G. Agarwal, M. Alam, Green tea: a boon for periodontal and general health, J. Indian Soc. Periodontol. 16(2) (2012) 161-167.

DOI: 10.4103/0972-124x.99256

[4] F. Wu, D. Bian, Y. Xia, Z. Gong, Q. Tan, J. Chen, Y. Dai, Identification of major active ingredients responsible for burn wound healing of Centella asiatica herbs, J. Evidence-Based Complementary Altern. Med. 2012 (2012) 1-13.

DOI: 10.1155/2012/848093

[5] C. Monton, S. Settharaksa, C. Luprasong, T. Songsak, An optimization approach of dynamic maceration of Centella asiatica to obtain the highest content of four centelloids by response surface methodology, Rev. Bras. Farmacogn. 29(2) (2019) 254-261.

DOI: 10.1016/j.bjp.2019.01.001

[6] W. Wisuitiprot, A. Somsiri, K. Ingkaninan, N. Waranuch, A novel technique for chitosan microparticle preparation using a water/silicone emulsion: green tea model, Int. J. Cosmet. Sci. 33(4) (2011) 351-358.

DOI: 10.1111/j.1468-2494.2010.00635.x

[7] W. Wisuitiprot, A. Somsiri, K. Ingkaninan, N. Waranuch, In vitro human skin permeation and cutaneous metabolism of catechins from green tea extract and green tea extract–loaded chitosan microparticles, Int. J. Cosmet. Sci. 33(6) (2011) 572-579.

DOI: 10.1111/j.1468-2494.2011.00673.x

[8] M.H. Rafamantanana, E. Rozet, G.E. Raoelison, K. Cheuk, S.U. Ratsimamanga, P. Hubert, J. Quetin-Leclercq, An improved HPLC-UV method for the simultaneous quantification of triterpenic glycosides and aglycones in leaves of Centella asiatica (L.) Urb (Apiaceae), J. Chromatogr. B: Anal. Technol. Biomed. Life Sci. 877(23) (2009) 2396-2402.

DOI: 10.1016/j.jchromb.2009.03.018

[9] Clinical and Laboratory Standards Institute, Method for antifungal disk diffusion susceptibility testing of yeasts, 3rd ed. M44. Clinical and Laboratory Standards Institute, Wayne, PA, (2018).

[10] Clinical and Laboratory Standards Institute, M11 Methods for antimicrobial susceptibility testing of anaerobic bacteria, 9th ed. M11. Clinical and Laboratory Standards Institute, Wayne, PA, (2018).

[11] E. Matuschek, D.F.J. Brown, G. Kahlmeter, Development of the EUCAST disk diffusion antimicrobial susceptibility testing method and its implementation in routine microbiology laboratories, Clin. Microbiol. Infect. 20(4) (2014) O255-O266.

DOI: 10.1111/1469-0691.12373

[12] A. Klančnik, S. Piskernik, B. Jeršek, S.S. Možina, Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts, J. Microbiol. Methods. 81(2) (2010) 121-126.

DOI: 10.1016/j.mimet.2010.02.004

[13] X. Xu, L. Xu, G. Yuan, Y. Wang, Y. Qu, M. Zhou, Synergistic combination of two antimicrobial agents closing each other's mutant selection windows to prevent antimicrobial resistance, Sci. Rep. 8(1) (2018).

DOI: 10.1038/s41598-018-25714-z

[14] M. Balouiri, M. Sadiki, S.K. Ibnsouda, Methods for in vitro evaluating antimicrobial activity: a review, J. Pharm. Anal. 6(2) (2016) 71-79.

DOI: 10.1016/j.jpha.2015.11.005

[15] J.C. Stockert, A. Blázquez-Castro, M. Cañete, R.W. Horobin, Á. Villanueva, MTT assay for cell viability: intracellular localization of the formazan product is in lipid droplets, Acta Histochem. 114(8) (2012) 785-796.

DOI: 10.1016/j.acthis.2012.01.006

[16] A. Grada, M. Otero-Vinas, F. Prieto-Castrillo, Z. Obagi, V. Falanga, Research techniques made simple: analysis of collective cell migration using the wound healing assay, J. Invest. Dermatol. 137(2) (2017) e11-e16.

DOI: 10.1016/j.jid.2016.11.020

[17] ICH, Q1A (R2) Stability testing of new drug substances and products, (2003).

[18] U. Markens, SGS, Conducting stability studies-recent changes to climatic zone IV, Life science, Technical bullet, 2009, pp.1-4.

[19] K.H. Row, Y. Jin, Recovery of catechin compounds from Korean tea by solvent extraction, Bioresour. Technol. 97(5) (2006) 790-793.

DOI: 10.1016/j.biortech.2005.04.001

[20] H. Barroso, R. Ramalhete, A. Domingues, S. Maci, Inhibitory activity of a green and black tea blend on Streptococcus mutans, J. Oral Microbiol. 10(1) (2018).

DOI: 10.1080/20002297.2018.1481322

[21] M. Chen, L. Zhai, M.C. Arendrup, In vitro activity of 23 tea extractions and epigallocatechin gallate against Candida species, Med. Mycol. 53(2) (2015) 194-198.

DOI: 10.1093/mmy/myu073

[22] W.C. Reygaert, Green tea catechins: their use in treating and preventing infectious diseases, BioMed Res. Int. 2018 (2018) 1-9.

DOI: 10.1155/2018/9105261

[23] P. Onklay, Development of antibacterial liquid soap containing assum tea extract, Naresuan University, Phitsanulok, (2009).

[24] I.A. Casalinuovo, R. Sorge, G. Bonelli, P. Di Francesco, Evaluation of the antifungal effect of EDTA, a metal chelator agent, on Candida albicans biofilm, Riv. Eur. Sci. Med. Farmacol. 21 (2017) 1413-1420.