Synthesis of Padina tetrastromatica Seaweed Mediated Silver Nanoparticles and its Toxicological Evaluation against Brine Shrimp Artemia

Abstract:

The present study evaluates the toxicity of AgNPs synthesised from Padina tetrastromatica (P-AgNPs) through Brine Shrimp Lethality Assay. The aqueous Padina seaweed extract was treated with AgNO₃ to synthesise P-AgNPs, and these NPs were then characterised by multiple analytical techniques. The UV-Vis spectra displayed characteristic SPR peaks of AgNPs at about 409.5 nm. The TEM and particle size distribution results verified the formation of polydisperse spherical P-AgNPs with a dominant size of around 48 nm. Zeta potential analysis indicates the moderate stability of P-AgNPs. The FTIR spectrum of P-AgNPs reveals the presence of organic functional groups, suggesting the involvements of seaweed organic matters in capping and stabilising AgNPs. Though the 24-h mortality test showed a dose-dependent increase in Artemia death, the results imply a non-toxic property in P-AgNPs (LC₅₀ value: 4300 mg/L; >1000 mg/L). These findings conclusively suggest the acute exposure to P-AgNPs has no substantial risk to marine organisms.

• Periodical: Materials Science Forum (Volume 1058)
• Pages: 9-14
• DOI: https://doi.org/10.4028/p-48s1r8
• Online since: April 2022
• Authors: Shin Yi Low, Jo Sze Lean, Ke Xin Yu, Ching Lee Wong, Jeck Fei Ng*
• Keywords: Acute Toxicity, Bio-Synthesis, Brine Shrimp, Padina tetrastromatica, Silver Nanoparticle

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