Polyethylene Glycol (PEG) Coated Magnetite Nanoparticles Prepared by Sol-Gel Method for Biotechnology Applications

Abstract:

Polyethylene glycol (PEG) coatings are developed for magnetite nanoparticles (NPs). The magnetic properties of superparamagnetic type, magnetite Fe₃O₄ nanoparticles are suitable for biosensing applications. Magnetic NPs were prepared by Co-precipitation method and oven dried. Using a Transmission Electron Microscope (TEM) and X-Ray Diffractometer (XRD), nanoparticles size and composition were found, including the presence of Fe₃O₄ peak. The magnetic properties are influenced by electron environments of the Fe³⁺ ions within the iron oxide structure. The magnetic properties were measured by Vibrating Sample Magnetometer (VSM), thus, the results of Fe₃O₄ NPs exhibited a high magnetic saturation (Ms) of 61.31 emu/g. In the case of PEG coated MNPs, confirmed by Fourier Transform Infrared Spectroscopy (FT-IR), a reduced Ms of 40.00 emu/g, which decreased further following surface modification with 3-aminopropyl triethoxysilane (NH₂) to 36.77 emu/g. The resulting size range of NPs of pure Fe₃O₄ NPs was 5-50 nm. In comparison, the PEG coated NPs were larger, 10-100 nm. In the part of protein binding and separation from solutions of bovine serum albumin (BSA) where investigated. This process will be beneficial to developing low cost sensors for biomolecules and biotechnologies in the future.

• Periodical: Materials Science Forum (Volume 1100)
• Pages: 65-73
• DOI: https://doi.org/10.4028/p-ph2iSg
• Online since: October 2023
• Authors: Panakamon Thonglor*, Darren Albutt
• Keywords: Co-Precipitation, Fe₃O₄ NPs, Magnetite Nanoparticles, Silica Coating

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