From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing

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Arg-Gly-Asp (RGD) peptidomimetics constructed on the tyrosine scaffold were equipped with an oligoethylene glycol (OEG) spacer-arm for surface grafting. Their in vitro activities as V3 integrin antagonists were in the range of 0.3-0.7 nM. Poly (ethylene terephthalate) (PET) membranes derivatized with peptidomimetics (50-120 pmol/cm2) allowed the adhesion and proliferation of endothelial cells (HSV) in serum-free medium. Iron oxide particles for MR Imaging grafted with peptidomimetics (2-3 molecules/particle of 20 nm diameter) were selectively targeted to activated tumour cells (Jurkat).

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Periodical:

Materials Science Forum (Volumes 638-642)

Main Theme:

Edited by:

T. Chandra, N. Wanderka, W. Reimers , M. Ionescu

Pages:

612-617

DOI:

10.4028/www.scientific.net/MSF.638-642.612

Citation:

J. Marchand-Brynaert "From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing", Materials Science Forum, Vols. 638-642, pp. 612-617, 2010

Online since:

January 2010

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$35.00

[20] We considered magnetite crystals (forming USPIO) with a volume of 0. 592 nm3 per unit cell. Surfaces of USPIO and unit cell are respectively 1256 nm2 and 0. 71 nm2, thus giving a number of 1769 unit cell faces displayed on one particle. Assuming the presence of 2 Fe atoms per unit cell face, there are 3538 Fe atoms on the surface of one particle. With an organic covering of 1. 6 %, we determine the number of 56 phophonate molecules per particle. The % of derivatization was calculated from the experimental (XPS) F/C atomic ratio as described in note.

DOI: 10.1016/0039-6028(91)90731-7

[14] with the formula: F/C = 3y / (3x + 44 y) for the grafting of Pept-EG6 (C3 for organic covering and C41N5F3 for Pept-EG6). We found: 3 x 4. 35 / [(3 x 95. 65) + (44 x 4. 35)] = 0. 027 (F/C experimental= 0. 028), giving 4. 35 % of derivatization (y) and 0. 0435 x 56 = 2. 43 molecules / particle.

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DOI: 10.1021/bm700841y

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