From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing

Jacqueline Marchand-Brynaert

doi:10.4028/www.scientific.net/MSF.638-642.612

Paper Titles

Bone Regeneration Based on Orientation of Biological Apatite (BAp) C-Axis in Osteopetrotic (op/op) Mice
p.588

Neutron Diffraction Residual Stress Evaluation and Numerical Modeling of Coating Obtained by PTA Process
p.594

A Dual Role of Copper on the Surface of Bone Implants
p.600

Haematological Performances of Carbon Coated PTFE by Plasma-Based Deposition
p.606

From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing
p.612

Fatigue and Fretting Fatigue Behavior of Metallic Biomaterials
p.618

Recent U.S. FDA Reclassification on the Regulation of Tissue Adhesives for Skin Approximation in Clinical Applications
p.624

Coarse Grain Modeling for Microtubule Mechanics
p.629

Mechanical Properties of Ti-Cr-Sn-Zr Alloys
p.635

HomeMaterials Science ForumMaterials Science Forum Vols. 638-642From Medicinal Chemistry to Functionalized...

From Medicinal Chemistry to Functionalized Biomaterials: Development of Graftable RGD-Peptitomimetics for Cell Adhesion and Cell Addressing

Abstract:

Arg-Gly-Asp (RGD) peptidomimetics constructed on the tyrosine scaffold were equipped with an oligoethylene glycol (OEG) spacer-arm for surface grafting. Their in vitro activities as V3 integrin antagonists were in the range of 0.3-0.7 nM. Poly (ethylene terephthalate) (PET) membranes derivatized with peptidomimetics (50-120 pmol/cm2) allowed the adhesion and proliferation of endothelial cells (HSV) in serum-free medium. Iron oxide particles for MR Imaging grafted with peptidomimetics (2-3 molecules/particle of 20 nm diameter) were selectively targeted to activated tumour cells (Jurkat).

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Materials Science Forum (Volumes 638-642)

Pages:

612-617

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.638-642.612

Citation:

Cite this paper

Online since:

January 2010

Authors:

Jacqueline Marchand-Brynaert

Keywords:

Arg-Gly-Asp, Endothelial Cell Cultivation, Iron Oxide Particle, PET Membrane, RGD-Peptidomimetic, Serum-Free Medium, Surface Grafting, T-Cell Targeting, α_Vβ₃ Integrin

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[20] We considered magnetite crystals (forming USPIO) with a volume of 0. 592 nm3 per unit cell. Surfaces of USPIO and unit cell are respectively 1256 nm2 and 0. 71 nm2, thus giving a number of 1769 unit cell faces displayed on one particle. Assuming the presence of 2 Fe atoms per unit cell face, there are 3538 Fe atoms on the surface of one particle. With an organic covering of 1. 6 %, we determine the number of 56 phophonate molecules per particle. The % of derivatization was calculated from the experimental (XPS) F/C atomic ratio as described in note.

DOI: 10.1016/0039-6028(91)90731-7

Google Scholar

[14] with the formula: F/C = 3y / (3x + 44 y) for the grafting of Pept-EG6 (C3 for organic covering and C41N5F3 for Pept-EG6). We found: 3 x 4. 35 / [(3 x 95. 65) + (44 x 4. 35)] = 0. 027 (F/C experimental= 0. 028), giving 4. 35 % of derivatization (y) and 0. 0435 x 56 = 2. 43 molecules / particle.

Google Scholar

[21] B. Manger, K. J. Hardy, A. Weiss, J. D. Stodo: J. Clinical Invest. Vol. 77 (1986) p.1501.

Google Scholar

[22] J. Marchand-Brynaert: Cardiovascular Pathology Vol. 17 (2008) p.276.

Google Scholar

[23] B. Driesschaert, N. Charlier, B. Gallez, J. Marchand-Brynaert: Bioorg. Med. Chem. Lett. Vol. 18 (2008) p.4291.

Google Scholar

[24] V. Pourcelle, S. Devouge, M. Garinot, V. Préat, J. Marchand-Brynaert: Biomacromolecules Vol. 8 (2007) p.3977.

DOI: 10.1021/bm700841y

Google Scholar