In this study, the effect of original thicknesses of plate, the thicknesses of plate after rolling and rolling reduction on the strength in 301 stainless steel was modeled by means of artificial neural network (ANN). The experimental data were collected to obtain training set and testing set. The normalization method was employed for avoiding over-fitting. The optimal ANN method architecture was determined by according to the trial and error procedure. The results of the ANN model were in good agreement with experimental data. As can be seen from the result, we believe that the neural network model can efficiently predict the relationship between mechanical properties and rolling reduction in 301 austenitic stainless steel.