Wood I-joists is a kind of wood-based engineering material which was widely used in wood construction. As it is in fact, the property of I-joists are influenced not only by shear stress but also by a part of normal stress when they are taking into application. In this study, the effects of different factors were evaluated on the tensile property of the wood I-joists. The factors include the depth of the tanks on the flanges, the amount of glue spread and adhesive type. The method of orthogonal test was used in the process of the experiment design, and tensile test were taken during the experiment at the aim of testing the property of the I-joists using a universal mechanical testing machine. Ultimately, it has been discovered that the factor of adhesive type is the most influential one in the three factors which had been listed. So this paper offers the optimal fabrication technology of the I-joists to make it is possible for the increase of reliability when wood I-joists are taken into application.