Effect of P(Bs-co-Cl) on Protein Content and Enzyme Activity in Earthworm

Abstract:

The natural soil-contact method was used to investigate the toxicity of poly (butylene succinate-co-caprolactone) (P(BS-co-CL)) copolymers with different number-average molecular weight (Mn) on the protein content, cellulose enzyme activity and peroxidase activity in earthworm extraction. The results showed that: during the first two weeks, both cellulose enzyme activity and peroxidase activity of earthworms treated by polymer (Mn is 8.0×10³), decreased with the increase of polymer content. In the polymer (Mn is 1.6×10⁴) group, in the end (28 and 42 d), cellulose enzyme and peroxidase activity decreased with the increase of the polymer concentration. However, the polymer (Mn is 2.8×10⁴) had no significant effect on the cellulose enzyme activity and peroxidase activity of earthworms. In addition, in the beginning (7 and 14 d) of the three treatments, the protein content increased with the raising of polymer concentration. Nevertheless, in the test treated by copolymers with high Mn, there was no significant changing.

• Periodical: Materials Science Forum (Volumes 743-744)
• Pages: 643-648
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.743-744.643
• Online since: January 2013
• Authors: Yong Lei Xing, Min Zhang, Cheng Tao Li, Chi Zhang, Wen Qing He, Ji Qing Song
• Keywords: Acute Ecotoxicity, Cellulose Enzyme, PBS-Based Copolymer, Peroxidise Activity, Protein

References

[1] X. Li, Y.R. Luo, M.X. Yun, et al., Effects of 1-methyl-3-octylimidazolium bromide on the anti-oxidant system of earthworm, J. Chemosphere. 78 (2010) 853-858.

DOI: 10.1016/j.chemosphere.2009.11.047

[2] Guidelines for Testing of Chemicals, Test 207: Earthworm acute toxicity tests, Organization for Economic Co-operation and Development (OECD), Paris, (1984).

DOI: 10.1787/9789264070042-en

[3] Y.J. Shi, Y.J. Shi, X. Wang, Comparative effects of lindane and deltamethrin on mortality, growth, and cellulase activity in earthworms (Eisenia fetida), J. Pesticide Biochemistry and Physiology. 89 (2007) 31-38.

DOI: 10.1016/j.pestbp.2007.02.005

[4] E. López, C. Arce, M.J. Oset-Gasque, et al., Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture, J. Free Radical Biology & Medicine. 40 (2006), 940-951.

DOI: 10.1016/j.freeradbiomed.2005.10.062

[5] Y. Song, L.S. Zhu, J. Wang, et al., DNA damage and effects on antioxidative enzymes in earthworm (Eisenia foetida) induced by atrazine, J. Soil Biology & Biochemistry. 41 (2011) 905-909.

DOI: 10.1016/j.soilbio.2008.09.009

[6] M. Zhang, W. Han, W.Q. Li, et al., Synthesis of poly( butylenes-succinate) with ε-caprolactone and lactic acid, J. Modern Chemical Industry. 27 (2007) 39-43.

[7] M. Zhang, K.Y. Su, C. Li, The effect of acute ecotoxicity of PBS-based copolymer and its simulated degradation products on earthworm Eisenia fetida, J. Ecology and Environmental Sciences. 19 (2010) 2425-2429.

[8] S.J. Wu, E.M. Wu, L.Q. Qiu, et al., Effects of phenanthrene on the mortality, growth, and anti-oxidant system of earthworms (Eisenia fetida) under laboratory conditions, J. Chemosphere. 83 (2011), 429-434.

DOI: 10.1016/j.chemosphere.2010.12.082

[9] Y.R. Luo, S.H. Wang, M.X. Yun, The toxic effects of ionic liquids on the activities of acetylcholinesterase and cellulase in earthworms, J. Chemosphere. 77 (2009) 313-318.

DOI: 10.1016/j.chemosphere.2009.07.026

[10] M.M. Bradford, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding, J. Analytical Biochemistry. 72 (1976) 248-254.

DOI: 10.1016/0003-2697(76)90527-3

[11] X.Y. Li, J. Zhou, M. Yu, et al., Toxic effects of 1-methyl-3-octylimidazolium bromide on the early embryonic development of the frog Rana nigromaculata, J. Ecotoxicol. Environ. Saf. 72 (2009) 552-556.

DOI: 10.1016/j.ecoenv.2007.11.002

[12] C. Jungnickel, J. Luczak, J. Ranke, et al., Micelle formation of imidazolium ionic liquids in aqueous solution, J. Colloids Surf. A. 316 (2008) 278-284.

DOI: 10.1016/j.colsurfa.2007.09.020

[13] Y.H. Gao, X.S. Sun, Z.J. Sun, et al., Toxic effects of enrofloxacin on growth rate and catalase activity in Eisenia fetida, J. Environmental Toxicology and Pharmacology. 26 (2008) 177-180.

DOI: 10.1016/j.etap.2008.03.004

[14] S. Liu, Q.X. Zhou, Y.Y. Wang, Ecotoxicological responses of the earthworm Eisenia fetida exposed to soil contaminated with HHCB, J. Chemosphere. 83 (2011) 1080-1086.

DOI: 10.1016/j.chemosphere.2011.01.046

[15] D.M. Xu, Y.Z. Wen, K.X. Wang, Effect of chiral differences of metolachlor and its (S)-isomer on their toxicity to earthworms, J. Ecotoxicology and Environmental Safety. 73 (2011) 1925-(1931).

DOI: 10.1016/j.ecoenv.2010.07.035

[16] R. Jayasekaraa, S. Sheridana, E. Lourbakosb, et al., Biodegradation and ecotoxicity evaluation of a bionolle and starch blend and its degradation products in compost, J. International Biodeterioration & Biodegradation. 51 (2003) 77-81.

DOI: 10.1016/s0964-8305(02)00090-2