Immobilized Enzyme Gox with Ferrite CoFe₂O₄ and Hybrid CoFe₂O₄/APTS

Abstract:

This work aims to evaluate the immobilization of glucose oxidase (GOx) with CoFe₂O₄ nanoparticles and hybrids CoFe₂O₄/APTS. The nanoparticles were synthesized by combustion reaction and silanized using 3-aminopropyltrimethoxysilane (APTS). The samples were characterized by XRD, FTIR, magnetic measurements and immobilization of GOx. The results indicated single phase of spinel CoFe₂O₄ and the addition of APTS did not alter the structure of the ferrite. The characteristic bands of spinel and characteristic bands were observed in the silane silanized sample proving the silanization of nanoparticles of CoFe₂O₄. The CoFe₂O₄ nanoparticles and the hybrid had saturation magnetization of 58.0 and 53.0 emu/g and coercivity of 1.14 kOe and 1.15, indicating that the silanization does not interfere with the magnetism of the particles. The immobilized GOx was obtained with values of 0.047 and 0.050 mg/g efficiency of 84.86 and 80.30% for CoFe₂O₄/APTS and CoFe₂O₄.

• Periodical: Materials Science Forum (Volume 820)
• Pages: 161-166
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.820.161
• Online since: June 2015
• Authors: Polyana Tarciana Araújo Santos*, P.M.A.G. Araújo, Elvia Leal, Izabelle L.T. de Albuquerque, Líbia S. Conrado, Ana Cristina Figueiredo de Melo Costa
• Keywords: 3-Aminopropyltrimethoxysilane, Hybrids, Immobilization of Gox

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