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HomeMaterials Science ForumMaterials Science Forum Vol. 847HRP Immobilization on Activated Carbon/Polyvinyl...

HRP Immobilization on Activated Carbon/Polyvinyl Formal Composite Materials for Degradation of Phenolic Wastewater

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Abstract:

A novel method was developed for degradation of phenolic waste water using horseradish peroxidase (HRP) immobilization on activated carbon/polyvinyl formal (AC/PVFM) composite materials in this study. The materials were black-sponge-like, soft and elastic, and had three-dimensional porous network structure. Optimum conditions of immobilized HRP carrier preparation technology was selected by orthogonal experiment. The enzymatic properties were explored and compared between immobilized and free enzyme. The optimum pH of immobilized HRP and free HRP were both 4, nevertheless, the immobilization of HRP increased the enzyme activity. The optimum temperature of immobilized HRP was 20°C which was lower than that of free HRP (25°C), therefore the immobilized HRP could resist to lower temperature. The K_m of immobilized HRP and free HRP were 0.23 mmol/L and 0.40 mmol/L, respectively. The lower K_m value of immobilized HRP indicated higher affinity to the substrate. These results show that HRP immobilized on AC/PVFM composite materials is promising in phenolic wastewater degradation.

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Periodical:

Materials Science Forum (Volume 847)

Pages:

256-264

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.847.256

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Online since:

March 2016

Authors:

Sai Nan Xu, Yong Chen

Keywords:

Activated Carbon/Polyvinyl Formal Composite Materials, Horseradish Peroxidase, Immobilization, Phenolic Wastewater Removal

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© 2016 Trans Tech Publications Ltd. All Rights Reserved

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