Evaluation of Antibacterial Activity of Chitosan Membranes Associated to Unripe Banana Peel

Abstract:

The treatment of skin wounds is a worldwide problem due to possible complications of healing process, such as infections, sepsis and even death. Current treatment strategies involves the use of dressings, bandages and topical application of antibiotic to the wound. An ideal dressing should protect against bacterial contamination, while covering the wound. The aim of this study was to investigate the antibacterial activity of chitosan membranes associated to banana peel for potential wound dressing application, since both materials have previously demonstrated antimicrobial properties. The active principles of banana peel were extracted by two methods: decoction and drying. Membranes were prepared by chitosan association with the extracts of first and third days of decoction and with different concentrations of dried extract, by the casting/solvent evaporation techniques. All obtained membranes were uniform, flexible and easily unmolded. Antibacterial activity studies were performed by the Surface Plating technique. Inhibition of bacterial growth was evaluated against two bacteria Escherichia coli and Staphylococcus aureus, usually present in skin wounds. All membranes were able to inhibit S. aureus e E. coli growth by contacting. In addition to contacting inhibition of E. coli growth, an inhibition halo of 0.5 cm diameter was also observed around the chitosan-unripe banana peel membrane prepared with the extract of first day of decoction.

• Periodical: Materials Science Forum (Volume 869)
• Pages: 859-863
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.869.859
• Online since: August 2016
• Authors: Patrícia Battaglini Franco, Leiliane Aparecida de Almeida, Rodrigo Fernando Costa Marques, Gunther Brucha, Maria Gabriela Nogueira Campos
• Keywords: Antibacterial Activity, Chitosan, Membranes, Unripe Banana Peel, Wound Dressing

References

[1] A.C.L. Campos, A.B. Branco and A.K. Groth: ABCD Arq. Cir. Dig. Vol. 20 (1) (2007), p.51.

DOI: 10.1590/s0102-67202007000100010

[2] L. Adam et al.: The New England Journal of Medicine Vol. 341 (10) (1999), p.738.

[3] W. Malagutti, C.T. Kakihara: Curativos, Eustomias e Dermatologia: uma Abordagem Multiprofissinal. (Martinari São Paulo, 2010).

[4] K. Kurita: Polym. Degrad. Stab. Vol. 59 (1998), p.117.

[5] M. Campos, L. Cordi, N. Duran and L. Mei: Macromol. Symp. Vol. 245 (2006), p.515.

[6] R.A. A Muzzarelli: Cell. Mol. Life Sci. Vol. 53 (1997), p.131.

[7] A. Sarasam, S.V. Madihally: Biomaterials Vol. 26 (2005), p.5500.

[8] E.I. Cadogan, C.H. Lee, S.R. Popuri et al.: Int. J. Polym. Mater. Po. Vol. 63 (2014), p.708.

[9] J. Berger, M. Reist, J.M. Mayer et al.: Eur. J. Pharm. Biopharm. Vol. 57 (2004), p.19.

[10] H.M. Maina, E.S. Heidi and M.H. Shagal: International Journal of Medicinal Plant Research Vol. 1 (3) (2012), p.20.

[11] P.B. Lino et al.: Brazilian Journal of Pharmacognosyv. Vol. 21 (3) (2011), p.491.

[12] J.M. Monteiro, U.P. Albuquerque, E.L. Araujo et al.: Química Nova Vol. 28 (5) (2005), p.892.

[13] H.W. Von Loesecke: Bananas. (2nd ed. Interscience, 1950).

[14] P.B. Franco et al. Chitosan membranes associated to Musa cavendishii for wound healing - Part I: Extraction of unripe banana peel. In: Encontro da SBPMAT, 2013, Campos do Jordão. Poster.. Campos de Jordão, SP, (2013).

[15] C. Pan, H. Rezaei and A. Soor: J. Exper. Microbiol. Immunol. Vol. 15 (2011), p.7.

[16] Y.J. Jeon and S.K. Kim: Carbohydr. Polym. Vol. 41 (2000), p.133.

[17] L. Y Zheng and J. Zhu: Carbohydr. Polym. Vol. 54 (2003), p.527.

[18] M. Kong, X.G. Xen, K. Xing et al: J. Food Microbiol. Vol. 144 (2010), p.51.

[19] H. Tan, R. Ma, C. Lin, Z. Liu et al.: Int. J. Mol. Sci Vol. 14. (2013), p.1854.

[20] L. H Li, J. C Deng, H.R. Deng, Z. L Liu et al.: Carbohydr. Res. Vol. 345 (2010), p.994.

[21] S. Zivanovic, S. Chi, A.F. Draughon: J. Food Sci. Vol. 70 (2009), p. M45.

[22] A.J. Friedman, J. Phan, D.O. Schairer1, J. Champer et al.: J. Invest. Dermatol. Vol. 133 (2013), p.1231.