Immobilization of Lipase onto Magnetic Nanoparticles for Enantiomer Selective Acetylation of Racemic 1-Phenylethylamine

Zhi Min Ou; Jia Ying Pan

doi:10.4028/www.scientific.net/MSF.960.128

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HomeMaterials Science ForumMaterials Science Forum Vol. 960Immobilization of Lipase onto Magnetic...

Immobilization of Lipase onto Magnetic Nanoparticles for Enantiomer Selective Acetylation of Racemic 1-Phenylethylamine

Abstract:

In this study, magnetic chitosan microspheres (Fe3O4-CTS) were prepared via chemical co-precipitation and cross-linked with lipase using glutaraldehyde to form Fe3O4-CTS-glutaraldehyde-lipase particles. The textural characteristics of Fe3O4-CTS-glutaraldehyde-lipase particles were assessed by scanning electron microscopy. The optimal immobilization conditions were 2.1 mg/mL lipase, 10 mg/mL Fe3O4-CTS-glutaraldehyde, pH 7.5, 30 °C, 2 h. The loading amount of lipase was 126.0 mg/g carrier, and the specific activity reached to 46.7 U/mg. Fe3O4-CTS-glutaraldehyde-lipase particles was used in resolution of racemic 1-phenylethylamine in a solvent-free system. The conversion and enantiomeric excess of (R)-N-(1-phenylethyl)acetamide reached 33.6% and 97%.

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Periodical:

Materials Science Forum (Volume 960)

Pages:

128-132

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.960.128

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Online since:

June 2019

Authors:

Zhi Min Ou*, Jia Ying Pan

Keywords:

(R)-N-(1-Phenylethyl)Acetamide, Enantiomer Selective Acetylation, Immobilization of Lipase, Magnetic Nanoparticles, Solvent-Free System

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