Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Abstract:

Pyrazinamine (PZA) is one of the most commonly prescribed anti-tuberculosis (anti-TB) drug due to its ability to significantly shorten the TB treatment period. However, excess PZA in the body causes hepatotoxicity and liver damage. This, therefore, calls for new methods for ensuring reliable dosing of the drug, which will differ from person to person due to interindividual differences in drug metabolism. A novel biosensor system for monitoring the metabolism of PZA was prepared with nanocomposite of multi-walled carbon nanotubes (MWCNTs), polyaniline (PANI) and cytochrome P450 3A4 (CYP3A4) electrochemically deposited on a glassy carbon electrode (GCE). The nanocomposite biosensor system exhibited enhanced electroactivity that is attributable to the catalytic effect of the incorporated MWCNTs. The biosensor had a sensitivity of 7.80 μA/μg mL^-1 PZA and a dynamic linear range of 4.92 160 ng/mL PZA.

• Periodical: Nano Hybrids (Volume 6)
• Pages: 59-73
• DOI: https://doi.org/10.4028/www.scientific.net/NH.6.59
• Online since: February 2014
• Authors: Unathi Sidwaba, Rachel F. Ajayi, Usisipho Feleni, Samantha Douman, Priscilla Gloria Lorraine Baker, Sibulelo L. Vilakazi, Robert Tshikhudo, Emmanuel Iwuoha*
• Keywords: Atomic Force Microscopy (AFM), Carbon Nanotube (CN), Cyclic Voltammetry, Cytochrome P450-2E1, Polyaniline, Pyrazinamide, Tuberculosis

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