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Preparation and Characterization of Terpenoid-Encapsulated PLGA Microparticles and its Antibacterial Activity against Enterococcus faecalis

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Abstract:

Exploration of natural compound for the treatment of dental-related problems are gaining of interest for enhancing therapeutic efficacy of the drugs delivery system. In this study, we have prepared terpenoid, which have been isolated from Myrmecodia pendens Merr & Perry from Papua Island, Indonesia, to be encapsulated in Polylactic-co-glycolic acid (PLGA), as the most widely used biodegradable polymer for biomedical applications, through one step single-emulsion method followed by subsequent coating by poly (vinyl alcohol) (PVA). The resultant of terpenoid-loaded PLGA microparticles were characterized systematically through scanning electron microscope and Fourier-transform infrared spectroscopy. In vitro drug release test was evaluated through dialysis method. Antibacterial test was conducted against Enterococcus faecalis as a model for persistent bacteria that causes root canal infections. The results showed that terpenoid-loaded PLGA microparticles were developed in spherical morphology with an average particle size of around 1-2μm. Terpenoid released from PLGA compartment at pH 6.5 and temperature of 37°C through a controlled-release profile mechanism with enhanced prolonged release. The bacterial assay result showed that terpenoid-loaded PLGA microparticles could reduce Enterococcus faecalis, effectively. Eventually, these result show that terpenoid-loaded PLGA microparticles as unique natural product-based extract could be developed as a potential naturally-based drug for dental-related diseases applications.

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Periodical:

Key Engineering Materials (Volume 829)

Pages:

263-269

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.829.263

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Online since:

December 2019

Authors:

Denny Nurdin*, Andri Hardiansyah, Elsy Rahimi Chaldun, Anti Khoerul Fikkriyah, Hendra Dian Adhita Dharsono, Dikdik Kurnia, Mieke Hemiawati Satari

Keywords:

Drug Delivery System, Enterococcus Faecalis, PLGA, Terpenoid

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© 2020 Trans Tech Publications Ltd. All Rights Reserved

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References

[1] V. Peciuliene, I. Balciuniene, H.M. Eriksen, M. Haapasalo, Isolation of Enterococcus faecalis in previously root filled canals in a lithuanian population. J Endod. 26 (2000) 593-595.

DOI: 10.1097/00004770-200010000-00004

Google Scholar

[2] H.H. Hancock, A. Sigurdson, M. Trope, J. Moiseiwitsch, Bacteria isolated after unsusccessful endodontic treatment in a north american population. Oral Surg Oral Med Oral Pathol. 91 (2001) 579-586.

DOI: 10.1067/moe.2001.113587

Google Scholar

[3] I. Portenier, T.M. Waltimo, M. Haapsalo, Enterococcus faecalis- the root canal survival and star in post treatment disease. Endodontic Topics. 6 (2003) 135-159.

DOI: 10.1111/j.1601-1546.2003.00040.x

Google Scholar

[4] C. Evanov, F. Lieweehr, T.B. Buxton, A.P. Joyce, Antibacterial efifacy of calcium hidroxide and chlorhexidine gluconate irrigant at 37°C and 46°C. J Endod. 30 (2004) 653-657.

DOI: 10.1097/01.don.0000121620.11272.22

Google Scholar

[5] B. Basrani, E. Pascon, S. Friedman. Efficacy of chlorhexidine-and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro, Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 96 (2003) 618-624.

DOI: 10.1016/s1079-2104(03)00166-5

Google Scholar

[6] R.E. Walton, E.M. River, Cleaning and Shaping, In: R.E. Walto, M. Torabinejad, Principles and Practice of Endodontic. third ed., W.B Saunders, Philadelphia, 2002, pp.206-238.

Google Scholar

[7] A.R. Ten Cate, Oral Histology: Development, Structure and Function, fifth ed., Mosby Inc., St. Louis M.O, 1998, p.155.

Google Scholar

[8] J.I. Ingle, L.K. Bakland. Endodontics, fifth ed., Bc Decker inc., London, 2002, pp.186-187.

Google Scholar

[9] D. Ricucci, J.F. Siqueira, A.L. Bate, T.R. Pitt Ford. Histologic investigation of root canal treated teeth with apical periodontitis: a retrospective study from 24 patients, J Endod. 3 (2009) 493-502.

DOI: 10.1016/j.joen.2008.12.014

Google Scholar

[10] I.N. Rocas, J.F. Siqueir, K.R.N Santos. Association of enterococcus faecalis with different forms of periradicular, J Endod. 30 (2004) 315-320.

DOI: 10.1097/00004770-200405000-00004

Google Scholar

[11] M.N. Hamsa, H.H. Mization. Potential of ant-nest plants as an alternative cancer treatment, J of Pharm Res. 6 (2012) 3063-3066.

Google Scholar

[12] A. Soeksmanto, M.A. Subroto, H. Wijaya, P. Simanjuntak. Anticancer activity for extracts of sarang semut plant (myrmecodia pendens) to HeLa and MCM-B2 cells, Pakistan J Biol. Sci. 13 (2010) 148-151.

DOI: 10.3923/pjbs.2010.148.151

Google Scholar

[13] T. Hertiani, E. Sasmito, Sumardi, M. Ulfah. Preliminary study on immunomodulatory effect of sarang semut plant (Myrmecodia pendens) to HeLa and MCM-B2 cells, Pakistan J Biol. Sci. 13 (2010) 136-141.

DOI: 10.3844/ojbsci.2010.136.141

Google Scholar

[14] L.I. Grossman, S. Oliet, C.E. Del Rio, Endodontic Practice, eleventh ed., Philadelphia, USA, (1995).

Google Scholar

[15] S.K. Ghosh, Functional Coating by Polymer Microencapsulation, Wiley-Vch Verlag GmbH and Co KgaA, Germany, (2006).

Google Scholar

[16] A.N. Ullman, Industrial Organic Chemicals 7, Willey-VCH, New York, (1989).

Google Scholar

[17] W. Wang. Microencapsulation using natural polysaccharides for drug delivery and cell implantation, J Mater Chem. 16 (2006) 3252–3267.

Google Scholar

[18] F. Danhier, E. Ansoren, J.M. Silva, R. Coco, A. Le Breton, V. Préat. PLGA-based nanoparticles: an overview of biomedical applications, Journal of Controlled Release. 161 (2012) 505-522.

DOI: 10.1016/j.jconrel.2012.01.043

Google Scholar

[19] U. Jana, A.K. Mohanty, S.L. Pal, P.K. Manna, G.P. Mohanta. Felodipine loaded PLGA nanoparticles: preparation, physicochemical characterization and in vivo toxicity study, J. Nano Convergence. 1 (2014) 31.

DOI: 10.1186/s40580-014-0031-5

Google Scholar

[20] D. Sun, N. Li, W. Zhang, E. Yang, Z. Mou, Z. Zhao, et. al. Quercetin-loaded PLGA nanoparticles: a highly effective antibacterial agent in vitro and anti-infection application in vivo, J. Nanopart Res. 18 (2015) 3.

DOI: 10.1007/s11051-015-3310-0

Google Scholar

[21] C. Fornaguer, N. Feiner-Gracia, G. Caldero, M.J. Garcia-Celma, C. Solans. Pharmacological aspects of galantamine for the treatment of Alzheimer's disease. J. Nanoscale. 7 (2015) 12076-12084.

DOI: 10.1039/c5nr03474d

Google Scholar

[22] C. Wischke, S.P. Schwendeman. Principles of encapsulating hydrophobic drugs in PLA/PLGA microparticles, International journal of pharmaceutics. 364 (2008) 298-327.

DOI: 10.1016/j.ijpharm.2008.04.042

Google Scholar

[23] J.E. Mc. Cormick JE, F.S Weine, J.D. Maggio. Tissue pH of developing periapical lesions in dogs, Journal of Endod. 9 (1983) 47-51.

DOI: 10.1016/s0099-2399(83)80074-0

Google Scholar

[24] D. Trombetta. Mechanisms of antibacterial action of three monoterpenes antimicrob. J. Agents Chemother. 49 (2005) 2474-2478.

DOI: 10.1128/aac.49.6.2474-2478.2005

Google Scholar

[25] T.C. Volgeson. Advances in drug delivery systems, J. Modern Drug Discovery. 4 (2001) 49-50,52.

Google Scholar

[26] A. Urzua, M.A. Rezende, C. Mascayano, L. Vasquez. A Structure-activity study of antibacterial diterpenoids, J. Molecules. 13 (2008) 882-891.

DOI: 10.3390/molecules13040822

Google Scholar

[27] Bush. Ceramic micro-particles synthesised using emulsion and sol-gel technology: an investigation into the controlled release of encapsulants and the tailoring of micro-particle size, Journal of Sol Gel Science and Technology. 3 (2006) 85-90.

DOI: 10.1007/s10971-004-5770-z

Google Scholar