Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material
To improve lipase activity and make the particulate carrier for practical application, lipase was conjugated to chitosan(Mwavg=80,000) by immine reaction. The lipase activity of conjugate was 93% of its initial activity at room temperature for 7 months, whereas the intact lipase activity decreased to 40%. And then, lipase-chitosan conjugate was intercalated within porous silica. The composite was characterized by X-ray diffraction, scanning electron microscopy, thermo gravimetric analysis. The Pore size was regulated in the range of 5~15nm. The maximum enzyme activity of lipase-chitosan conjugate needs the structure with 15nm pore of mesoporous silica. The resultant composite was found to have the free flowing property and keep up inner lipase activity.
Byung Tae Ahn, Hyeongtag Jeon, Bo Young Hur, Kibae Kim and Jong Wan Park
S. S. Kwon et al., "Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material", Solid State Phenomena, Vols. 124-126, pp. 1717-1720, 2007