Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material

Abstract:

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To improve lipase activity and make the particulate carrier for practical application, lipase was conjugated to chitosan(Mwavg=80,000) by immine reaction. The lipase activity of conjugate was 93% of its initial activity at room temperature for 7 months, whereas the intact lipase activity decreased to 40%. And then, lipase-chitosan conjugate was intercalated within porous silica. The composite was characterized by X-ray diffraction, scanning electron microscopy, thermo gravimetric analysis. The Pore size was regulated in the range of 5~15nm. The maximum enzyme activity of lipase-chitosan conjugate needs the structure with 15nm pore of mesoporous silica. The resultant composite was found to have the free flowing property and keep up inner lipase activity.

Info:

Periodical:

Solid State Phenomena (Volumes 124-126)

Edited by:

Byung Tae Ahn, Hyeongtag Jeon, Bo Young Hur, Kibae Kim and Jong Wan Park

Pages:

1717-1720

DOI:

10.4028/www.scientific.net/SSP.124-126.1717

Citation:

S. S. Kwon et al., "Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material", Solid State Phenomena, Vols. 124-126, pp. 1717-1720, 2007

Online since:

June 2007

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Price:

$35.00

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