Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material

Sun Sang Kwon; Sang Hoon Jeon; Jeong Kuk Shon; Duck Hee Kim; Ih Seop Chang; Ji Man Kim

doi:10.4028/www.scientific.net/SSP.124-126.1717

Paper Titles

Measurement of Mechanical Properties for Thin Film Using Visual Image Tracing Method
p.1701

Estimation for Nd-Fe-B Melt-Spun Powder Quality
p.1705

Encapsulation of Municipal Solid Waste Incineration Bottom Ash to Immobilize Cu and Pb via Carbonation Reaction
p.1709

Modification of V₂O₅-WO₃/TiO₂ Catalysts Supported on SiC Filter for NO Reduction at Low Temperature
p.1713

Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material
p.1717

Fabrication of Lotus-Type Porous NiAl and Ni₃Al Intermetallic Compounds
p.1721

Pore Morphology of Lotus-Type Porous Copper Fabricated by Continuous Casting Technique
p.1725

Gold Nanowire-Network Structure Prepared from Metallic Gold Nanowires Deposited in Polycarbonate Template Membrane
p.1729

Nanoporous SiC Membrane Derived from Preceramic Polymer
p.1733

HomeSolid State PhenomenaSolid State Phenomena Vols. 124-126Preparation and Stabilization of Chitosan-Lipase...

Preparation and Stabilization of Chitosan-Lipase Composite within Mesoporous Silica Material

Abstract:

To improve lipase activity and make the particulate carrier for practical application, lipase was conjugated to chitosan(Mwavg=80,000) by immine reaction. The lipase activity of conjugate was 93% of its initial activity at room temperature for 7 months, whereas the intact lipase activity decreased to 40%. And then, lipase-chitosan conjugate was intercalated within porous silica. The composite was characterized by X-ray diffraction, scanning electron microscopy, thermo gravimetric analysis. The Pore size was regulated in the range of 5~15nm. The maximum enzyme activity of lipase-chitosan conjugate needs the structure with 15nm pore of mesoporous silica. The resultant composite was found to have the free flowing property and keep up inner lipase activity.

You might also be interested in these eBooks

Advances in Nanomaterials and Processing

View Preview

Info:

Periodical:

Solid State Phenomena (Volumes 124-126)

Pages:

1717-1720

DOI:

https://doi.org/10.4028/www.scientific.net/SSP.124-126.1717

Citation:

Cite this paper

Online since:

June 2007

Authors:

Sun Sang Kwon, Sang Hoon Jeon, Jeong Kuk Shon, Duck Hee Kim, Ih Seop Chang, Ji Man Kim

Keywords:

Chitosan (CS), Lipase, Mesoporous Silica, Stabilisation

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Iqbal Gill, Antonio Ballesteros : Bioencapsulation within synthetic polymers(part2). Vol. 18 (2000) p.469.

Google Scholar

[2] Iqbal Gill.: Bio-doped nanocomposite polymers. Vol. 13 (2001) p.3404.

Google Scholar

[3] Giovanni S., Riccardo NB., Daniele C., Pier GP. : Enzyme and Microbial Technology, Vol. 28, (2001) p.427.

Google Scholar

[4] Birute S., Vida B., Benediktas J., Irena B., Liucija M. : Biotechnol. Appl. Biochem. Vol. 36, (2002) p.47.

Google Scholar

[5] Ansil Dyal, Katja Loos, Mayumi Noto, Seung W. Chang : J. Am. Chem. Soc.: Vol. 125 (2003), p.1684.

Google Scholar

[6] Pirkko Helistö, Timo Korpela, : Enzyme and Microbial Technology. 23, (1998) p.113 2θθθθ 0 1 2 3 4 5 6 Intensity 0 5000 10000 15000 20000 TMB / surfactant 0. 0 1. 0 1. 5 1. 75 2. 0.

Google Scholar